Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein

H. E. Horng, S. Y. Yang, Chin Yih Hong, C. M. Liu, P. S. Tsai, H. C. Yang, C. C. Wu

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.

Original languageEnglish
Article number252506
JournalApplied Physics Letters
Volume88
Issue number25
DOIs
Publication statusPublished - Jun 19 2006

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proteins
nanoparticles
sensitivity
gradiometers
markers
enzymes
interference
magnetization

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein. / Horng, H. E.; Yang, S. Y.; Hong, Chin Yih; Liu, C. M.; Tsai, P. S.; Yang, H. C.; Wu, C. C.

In: Applied Physics Letters, Vol. 88, No. 25, 252506, 19.06.2006.

Research output: Contribution to journalArticle

Horng, H. E. ; Yang, S. Y. ; Hong, Chin Yih ; Liu, C. M. ; Tsai, P. S. ; Yang, H. C. ; Wu, C. C. / Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein. In: Applied Physics Letters. 2006 ; Vol. 88, No. 25.
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