Biofabrication of cell-laden gelatin methacryloyl hydrogels with incorporation of silanized hydroxyapatite by visible light projection

Jimmy Jiun Ming Su, Chih Hsin Lin, Hsuan Chen, Shyh Yuan Lee, Yuan Min Lin

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Gelatin methacryloyl (GelMA) hydrogel is a photopolymerizable biomaterial widely used for three-dimensional (3D) cell culture due to its high biocompatibility. However, the drawback of GelMA hydrogel is its poor mechanical properties, which may compromise the feasibility of biofabrication techniques. In this study, a cell-laden GelMA composite hydrogel with a combination incorporating silanized hydroxyapatite (Si-HAp) and a simple and harmless visible light crosslinking system for this hydrogel were developed. The incorporation of Si-HAp into the GelMA hydrogel enhanced the mechanical properties of the composite hydrogel. Moreover, the composite hydrogel exhibited low cytotoxicity and promoted the osteogenic gene expression of embedded MG63 cells and Human bone marrow mesenchymal stem cells (hBMSCs). We also established a maskless lithographic method to fabricate a defined 3D structure under visible light by using a digital light processing projector, and the incorporation of Si-HAp increased the resolution of photolithographic hydrogels. The GelMA-Si-HAp composite hydrogel system can serve as an effective biomaterial in bone regeneration.

Original languageEnglish
Article number2354
JournalPolymers
Volume13
Issue number14
DOIs
Publication statusPublished - Jul 2 2021

Keywords

  • 3D printing
  • Digital light processing projection
  • Gelatin methacrylate
  • Hydroxyapatite
  • Mesenchymal stem cells
  • Silanization

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

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