Bioengineered human corneal endothelium for transplantation

Jui Yang Lai, Ko Hua Chen, Wen-Ming Hsu, Ging Ho Hsiue, Yen Hsien Lee

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Objective: To investigate whether the bioengineered human corneal endothelial cell (HCEC) monolayers harvested from thermoresponsive culture supports could be used as biological tissue equivalents. Methods: Untransformed adult HCECs derived from eye bank corneas were cultivated on a thermoresponsive poly-N-isopropylacrylamide-grafted surface for 3 weeks at 37°C. Confluent cell cultures with a phenotype and cell density similar to HCECs in vivo were detached as a laminated sheet by lowering the culture temperature to 20°C. In vitro characteristics of theHCECsheets were determined evaluating their viability and by scanning electron microscopy, immunohistochemistry, and histological studies. Results: After separation from culture surfaces via a thermal stimulus, the HCEC sheets remained viable. Polygonal cell morphology and multiple cellular interconnections were observed throughout the HCEC sheets. Immunolocalization of zonula occludens-1 and Na+,K +-adenosine triphosphatase (ATPase) indicated the formation of tight junctions and the distribution of ionic pumps at the cell boundary. In addition, we ascertained that cultured HCECs have a monolayered architecture that mimics native corneal endothelium. Conclusion: These data suggest that a well-organized and functional HCEC monolayer can feasibly be used as tissue equivalents for replacing compromised endothelium. Clinical Relevance: Bioengineered human corneal endothelium fabricated from thermoresponsive supports can potentially offer a new therapeutic strategy for corneal endothelial cell loss.

Original languageEnglish
Pages (from-to)1441-1448
Number of pages8
JournalArchives of Ophthalmology
Volume124
Issue number10
DOIs
Publication statusPublished - Oct 16 2006
Externally publishedYes

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Corneal Endothelium
Corneal Transplantation
Endothelial Cells
Tight Junctions
Corneal Endothelial Cell Loss
Eye Banks
Electron Scanning Microscopy
Cornea
Endothelium
Adenosine Triphosphatases
Cell Culture Techniques
Cell Count
Hot Temperature
Immunohistochemistry
Phenotype
Temperature
3-hydroxycholest-7-ene-14-carbaldehyde

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Bioengineered human corneal endothelium for transplantation. / Lai, Jui Yang; Chen, Ko Hua; Hsu, Wen-Ming; Hsiue, Ging Ho; Lee, Yen Hsien.

In: Archives of Ophthalmology, Vol. 124, No. 10, 16.10.2006, p. 1441-1448.

Research output: Contribution to journalArticle

Lai, Jui Yang ; Chen, Ko Hua ; Hsu, Wen-Ming ; Hsiue, Ging Ho ; Lee, Yen Hsien. / Bioengineered human corneal endothelium for transplantation. In: Archives of Ophthalmology. 2006 ; Vol. 124, No. 10. pp. 1441-1448.
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