Biodegradable vancomycin-eluting poly[(d,l)-lactide-co-glycolide] nanofibres for the treatment of postoperative central nervous system infection

Yuan Yun Tseng, Yi Chuan Wang, Chen Hsing Su, Shih Jung Liu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The incidence of postoperative central nervous system infection (PCNSI) is higher than 5%-7%. Successful management of PCNSI requires a combined therapy of surgical debridement and long-term antibiotic treatment. In this study, Duraform soaked in a prepared bacterial solution was placed on the brain surface of rats to induce PCNSI. Virgin poly[(d,l)-lactide-co-glycolide] (PLGA) nanofibrous membranes (vehicle-control group) and vancomycin-eluting PLGA membranes (vancomycin-nanofibres group) were implanted. The wound conditions were observed and serial brain MRI and pathology examinations were performed regularly. PCNSI was consistently induced in a single, simple step. In the vehicle-control group, most rats died within 1 week, and the survival rate was low (odds ratio = 0.0357, 95% confidence interval = 0.0057-0.2254). The wounds and affected cerebral tissues necrosed with purulence and increased in mass from the resulting PCNSI volumes. Initially, the mean PCNSI volumes showed no significant difference between the two groups. The PCNSI volume in the rats in the vancomycin-nanofibres group significantly decreased (P <0.01), and the wound appearance was excellent. Pathologic examinations revealed that the necrosis and leukocyte infiltration area decreased considerably. The experimental results suggest that vancomycin-eluting PLGA nanofibres are favourable candidates for treating PCNSI after surgical debridement.

Original languageEnglish
Article number7849
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 2015

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Nanofibers
Central Nervous System Infections
Vancomycin
Debridement
Wounds and Injuries
Control Groups
Membranes
dilactide
Brain
Leukocytes
Necrosis
Odds Ratio
Confidence Intervals
Pathology
Anti-Bacterial Agents
Incidence

ASJC Scopus subject areas

  • General

Cite this

Biodegradable vancomycin-eluting poly[(d,l)-lactide-co-glycolide] nanofibres for the treatment of postoperative central nervous system infection. / Tseng, Yuan Yun; Wang, Yi Chuan; Su, Chen Hsing; Liu, Shih Jung.

In: Scientific Reports, Vol. 5, 7849, 2015.

Research output: Contribution to journalArticle

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