Abstract
A new type of chondroitin sulfate (ChS)-chitosan (ChI) polyelectrolyte complex (PEC) sponge for controllable basic fibroblast growth factor (bFGF) release has been prepared by the methods of homogenizing interpolyelectrolyte complex. Since chondroitin sulfate is a recognized bFGF-binding glycosaminoglycan, and is very soluble in water, the ChS-ChI PEC sponges were crosslinked with glutaraldehyde, EDC/NHS and calcium ions respectively to prepare covalent- and ioniccrosslinked polymer networks. The stability, and in vitro enzymatic degradability and cytotoxicity of the glutaraldehyde-, EDC- and Ca2+-crosslinked ChS-ChI PEC sponges were all investigated in this study. The resuls showed that crosslinking improved the stability of prepared ChS-ChI PEC sponges, and provided more protective effect against the dissolution and enzymatic hydrolysis of fixed chondroitin sulfate, as compared to their non-crosslinked counterpart. However, we found that the ionic-crosslinking of ChS-ChI PEC sponges with calcium ions impaired the cell proliferation, suggested that cytotoxicity might be induced by calcium ions. To evaluate the conjugation interaction of bFGF with the ChS-ChI PEC sponges, the effects for the adsorption of bFGF to original and crosslinked-ChS-Chl PEC sponges were examined by ELISA studies. The bFGFconjugated ChS-ChI PEC sponges demonstrated different bFGF release pattern by the variation of crosslinking methods in a concentration-dependent way. The initial burst release could be eliminated due to the ChS-ChI interpolyelectrolyte complex and the crosslinking effect. These results suggest that the modified ChS-ChI PEC sponges may be beneficial to control the bFGF-releasing thus enhances the application potential of the bFGF-conjugated biomaterial for wound repair or tissue engineering.
| Original language | English |
|---|---|
| Title of host publication | Transactions - 7th World Biomaterials Congress |
| Pages | 1511 |
| Number of pages | 1 |
| Publication status | Published - 2004 |
| Externally published | Yes |
| Event | Transactions - 7th World Biomaterials Congress - Sydney, Australia Duration: May 17 2004 → May 21 2004 |
Other
| Other | Transactions - 7th World Biomaterials Congress |
|---|---|
| Country | Australia |
| City | Sydney |
| Period | 5/17/04 → 5/21/04 |
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ASJC Scopus subject areas
- Engineering(all)
Cite this
Biodegradable chitosan-chondroitin sulfate sponge for the controlled released of basic fibroblast growth factor. / Mi, F. L.; Wu, Y. B.; Wang, P. S.; Peng, C. K.; Shyu, S. S.; Yu, S. H.; Huang, M. F.
Transactions - 7th World Biomaterials Congress. 2004. p. 1511.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Biodegradable chitosan-chondroitin sulfate sponge for the controlled released of basic fibroblast growth factor
AU - Mi, F. L.
AU - Wu, Y. B.
AU - Wang, P. S.
AU - Peng, C. K.
AU - Shyu, S. S.
AU - Yu, S. H.
AU - Huang, M. F.
PY - 2004
Y1 - 2004
N2 - A new type of chondroitin sulfate (ChS)-chitosan (ChI) polyelectrolyte complex (PEC) sponge for controllable basic fibroblast growth factor (bFGF) release has been prepared by the methods of homogenizing interpolyelectrolyte complex. Since chondroitin sulfate is a recognized bFGF-binding glycosaminoglycan, and is very soluble in water, the ChS-ChI PEC sponges were crosslinked with glutaraldehyde, EDC/NHS and calcium ions respectively to prepare covalent- and ioniccrosslinked polymer networks. The stability, and in vitro enzymatic degradability and cytotoxicity of the glutaraldehyde-, EDC- and Ca2+-crosslinked ChS-ChI PEC sponges were all investigated in this study. The resuls showed that crosslinking improved the stability of prepared ChS-ChI PEC sponges, and provided more protective effect against the dissolution and enzymatic hydrolysis of fixed chondroitin sulfate, as compared to their non-crosslinked counterpart. However, we found that the ionic-crosslinking of ChS-ChI PEC sponges with calcium ions impaired the cell proliferation, suggested that cytotoxicity might be induced by calcium ions. To evaluate the conjugation interaction of bFGF with the ChS-ChI PEC sponges, the effects for the adsorption of bFGF to original and crosslinked-ChS-Chl PEC sponges were examined by ELISA studies. The bFGFconjugated ChS-ChI PEC sponges demonstrated different bFGF release pattern by the variation of crosslinking methods in a concentration-dependent way. The initial burst release could be eliminated due to the ChS-ChI interpolyelectrolyte complex and the crosslinking effect. These results suggest that the modified ChS-ChI PEC sponges may be beneficial to control the bFGF-releasing thus enhances the application potential of the bFGF-conjugated biomaterial for wound repair or tissue engineering.
AB - A new type of chondroitin sulfate (ChS)-chitosan (ChI) polyelectrolyte complex (PEC) sponge for controllable basic fibroblast growth factor (bFGF) release has been prepared by the methods of homogenizing interpolyelectrolyte complex. Since chondroitin sulfate is a recognized bFGF-binding glycosaminoglycan, and is very soluble in water, the ChS-ChI PEC sponges were crosslinked with glutaraldehyde, EDC/NHS and calcium ions respectively to prepare covalent- and ioniccrosslinked polymer networks. The stability, and in vitro enzymatic degradability and cytotoxicity of the glutaraldehyde-, EDC- and Ca2+-crosslinked ChS-ChI PEC sponges were all investigated in this study. The resuls showed that crosslinking improved the stability of prepared ChS-ChI PEC sponges, and provided more protective effect against the dissolution and enzymatic hydrolysis of fixed chondroitin sulfate, as compared to their non-crosslinked counterpart. However, we found that the ionic-crosslinking of ChS-ChI PEC sponges with calcium ions impaired the cell proliferation, suggested that cytotoxicity might be induced by calcium ions. To evaluate the conjugation interaction of bFGF with the ChS-ChI PEC sponges, the effects for the adsorption of bFGF to original and crosslinked-ChS-Chl PEC sponges were examined by ELISA studies. The bFGFconjugated ChS-ChI PEC sponges demonstrated different bFGF release pattern by the variation of crosslinking methods in a concentration-dependent way. The initial burst release could be eliminated due to the ChS-ChI interpolyelectrolyte complex and the crosslinking effect. These results suggest that the modified ChS-ChI PEC sponges may be beneficial to control the bFGF-releasing thus enhances the application potential of the bFGF-conjugated biomaterial for wound repair or tissue engineering.
UR - http://www.scopus.com/inward/record.url?scp=13844266355&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13844266355&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:13844266355
SN - 1877040193
SN - 9781877040191
SP - 1511
BT - Transactions - 7th World Biomaterials Congress
ER -