Biochemical comparison of γ-crystallins from duck and frog eye lenses

Shyh Horng Chiou, Wen Chang Chang, Jane Kuo, Fu Ming Pan, Tung Bin Lo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A biochemical comparison has been made on the crystallins isolated from duck and frog lenses. Gel-permeation chromatography of lens homogenates from both classes on Fractogel TSK HW-55(S) revealed a homogeneous trimeric protein of 120 kDa in the duck lenses and a monomeric protein of 39 kDa in the frog lenses. Both crystallin fractions consist only of an approx. 38-kDa polypeptide in their subunit structures as determined by SDS gel electrophoresis. These two crystalline were compared with respect to their native molecular masses, subunit structures, peptide mapping and amino acid compositions in order to establish the identity of each crystallin. We have found differences in the protein structures of these two crystallins despite some degree of similarity in their amino acid compositions.

Original languageEnglish
Pages (from-to)219-222
Number of pages4
JournalFEBS Letters
Volume196
Issue number2
DOIs
Publication statusPublished - Feb 17 1986
Externally publishedYes

Fingerprint

Crystalline Lens
Crystallins
Ducks
Anura
Lenses
Amino Acids
Peptides
Proteins
Peptide Mapping
Gel permeation chromatography
Molecular mass
Electrophoresis
Chemical analysis
Gel Chromatography
Gels
Crystalline materials

Keywords

  • Duck lens)Amino acid composition Peptide mapping Sequence homology
  • γ-Crystallin(Frog lens

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Biochemical comparison of γ-crystallins from duck and frog eye lenses. / Chiou, Shyh Horng; Chang, Wen Chang; Kuo, Jane; Pan, Fu Ming; Lo, Tung Bin.

In: FEBS Letters, Vol. 196, No. 2, 17.02.1986, p. 219-222.

Research output: Contribution to journalArticle

Chiou, Shyh Horng ; Chang, Wen Chang ; Kuo, Jane ; Pan, Fu Ming ; Lo, Tung Bin. / Biochemical comparison of γ-crystallins from duck and frog eye lenses. In: FEBS Letters. 1986 ; Vol. 196, No. 2. pp. 219-222.
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