Biochemical and structural analysis of the IgE binding sites on Ara h1, an abundant and highly allergenic peanut protein

David S. Shin, Cesar M. Compadre, Soheila J. Maleki, Randall A. Kopper, Hugh Sampson, Shau K. Huang, A. Wesley Burks, Gary A. Bannon

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

Allergy to peanut is a significant IgE-mediated health problem because of the high prevalence, potential severity, and chronicity of the reaction. Ara h1, an abundant peanut protein, is recognized by serum IgE from >90% of peanut-sensitive individuals. It has been shown to belong to the vicilin family of seed storage proteins and to contain 23 linear IgE binding epitopes. In this communication, we have determined the critical amino acids within each of the IgE binding epitopes of Arahi that are important for immunoglobulin binding. Surprisingly, substitution of a single amino acid within each of the epitopes led to loss of IgE binding. In addition, hydrophobic residues appeared to be most critical for IgE binding. The position of each of the IgE binding on a homology-based molecular model of Ara hi showed that they were clustered into two main regions, despite their more even distribution in the primary sequence. Finally, we have shown that Ara hi forms a stable trimer by the use of a reproducible fluorescence assay. This information will be important in studies designed to reduce the risk of peanut-induced anaphylaxis by lowering the IgE binding capacity of the allergen.

Original languageEnglish
Pages (from-to)13753-13759
Number of pages7
JournalJournal of Biological Chemistry
Volume273
Issue number22
DOIs
Publication statusPublished - May 29 1998
Externally publishedYes

Fingerprint

Structural analysis
Immunoglobulin E
Binding Sites
Proteins
Epitopes
Peanut Hypersensitivity
Seed Storage Proteins
Allergies
Amino Acids
Molecular Models
Arachis
Anaphylaxis
Medical problems
Allergens
Immunoglobulins
Assays
Substitution reactions
Fluorescence
Communication
Health

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Biochemical and structural analysis of the IgE binding sites on Ara h1, an abundant and highly allergenic peanut protein. / Shin, David S.; Compadre, Cesar M.; Maleki, Soheila J.; Kopper, Randall A.; Sampson, Hugh; Huang, Shau K.; Burks, A. Wesley; Bannon, Gary A.

In: Journal of Biological Chemistry, Vol. 273, No. 22, 29.05.1998, p. 13753-13759.

Research output: Contribution to journalArticle

Shin, David S. ; Compadre, Cesar M. ; Maleki, Soheila J. ; Kopper, Randall A. ; Sampson, Hugh ; Huang, Shau K. ; Burks, A. Wesley ; Bannon, Gary A. / Biochemical and structural analysis of the IgE binding sites on Ara h1, an abundant and highly allergenic peanut protein. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 22. pp. 13753-13759.
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