Biochemical and biological properties of an α1-antitrypsin concentrate

T. Burnouf, J. Constans, A. Clerc, J. Descamps, L. Martinache, M. Goudemand

Research output: Contribution to journalArticle

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Abstract

α1-Antitrypsin (AAT) has been purified from human plasma supernatant A (equivalent to COHN fraction II + III) by a large-scale chromatographic procedure involving anion-exchange adsorption on DEAE Sepharose CL-6B fast flow and size-exclusion chromatography on Sephacryl S-200. Before freeze-drying, the liquid concentrate was heat-treated at 60°C for 10 h to reduce the risk of transmission of blood-borne viral diseases. Using this procedure, AAT is recovered with 80-90% purity in 65-75% yield from supernatant A. The heterogeneity of AAT is preserved across the purification steps. In addition, purified AAT exhibits inhibitory activities against trypsin and elastase equivalent to that of the serum protein. The mean association rate constant for elastase was found as high as 2.15 x 105 M-1 s-1. Thus, purifying active AAT from supernatant A contributes to improving the availability of this protein which may be potentially useful in the treatment of hereditary emphysema.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalVox Sanguinis
Volume52
Issue number4
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Pancreatic Elastase
Freeze Drying
Emphysema
Virus Diseases
Trypsin
Adsorption
Gel Chromatography
Anions
Blood Proteins
Hot Temperature
Proteins
Therapeutics
sephacryl S 200
DEAE-Sepharose CL-6B

ASJC Scopus subject areas

  • Hematology

Cite this

Burnouf, T., Constans, J., Clerc, A., Descamps, J., Martinache, L., & Goudemand, M. (1987). Biochemical and biological properties of an α1-antitrypsin concentrate. Vox Sanguinis, 52(4), 291-297.

Biochemical and biological properties of an α1-antitrypsin concentrate. / Burnouf, T.; Constans, J.; Clerc, A.; Descamps, J.; Martinache, L.; Goudemand, M.

In: Vox Sanguinis, Vol. 52, No. 4, 1987, p. 291-297.

Research output: Contribution to journalArticle

Burnouf, T, Constans, J, Clerc, A, Descamps, J, Martinache, L & Goudemand, M 1987, 'Biochemical and biological properties of an α1-antitrypsin concentrate', Vox Sanguinis, vol. 52, no. 4, pp. 291-297.
Burnouf T, Constans J, Clerc A, Descamps J, Martinache L, Goudemand M. Biochemical and biological properties of an α1-antitrypsin concentrate. Vox Sanguinis. 1987;52(4):291-297.
Burnouf, T. ; Constans, J. ; Clerc, A. ; Descamps, J. ; Martinache, L. ; Goudemand, M. / Biochemical and biological properties of an α1-antitrypsin concentrate. In: Vox Sanguinis. 1987 ; Vol. 52, No. 4. pp. 291-297.
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