Abstract

Background: Parkinson's disease, affecting at least 1% of population older than 65 years, is the most common neurodegenerative movement disorder. Up to now, no evidence has demonstrated that biochemical changes in CSF occur preceding the onset of Parkinson's symptoms. In this study, we tested the hypothesis that biochemical changes in CSF precede behavioral deficits in Parkinsonian animals. Methods: We infused different doses of 6-OHDA into the MFB of rats bilaterally and examined the animals' movement behaviors, biochemical alterations in CSF, and dopaminergic neuronal number in the SNpc 1 week later. Results: Our results indicated that animals with over 70% dopaminergic neuronal loss in the SNpc exhibited behavioral bradykinesia and rigidity, and a decrease of HVA in CSF. In contrast, animals with about 42% dopaminergic neuronal loss in the SNpc showed normal movement behaviors, but displayed a drastic decline of HVA in CSF. Furthermore, the number of dopaminergic neurons in the SNpc was positively correlated with the HVA level in CSF. Conclusions: Our findings demonstrate that biochemical alteration in CSF foreruns behavioral deficits and the HVA level in CSF is positively correlated with the number of dopaminergic neurons in the SNpc of Parkinsonian rats induced by 6-OHDA. Our results strongly suggest that additional studies are needed to evaluate usefulness of monitoring the HVA level in CSF for early detection of the loss of dopaminergic neurons in the SNpc that precedes the onset of Parkinsonian symptoms in humans.

Original languageEnglish
Pages (from-to)S55-S65
JournalSurgical Neurology
Volume72
Issue numberSUPPL. 2
DOIs
Publication statusPublished - Dec 2009

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Oxidopamine
Dopaminergic Neurons
Cerebrospinal Fluid
Hypokinesia
Animal Behavior
Movement Disorders
Neurodegenerative Diseases
Parkinson Disease
Population

Keywords

  • 6-Hydroxydopamine
  • CSF
  • Parkinson's disease
  • Parkinsonism
  • Substantia nigra
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Biochemical alteration in cerebrospinal fluid precedes behavioral deficits in Parkinsonian rats induced by 6-hydroxydopamine. / Lin, Jia-Wei; Shih, Chwen-Ming; Chen, Yen-Chou; Lin, Chien-Min; Tsai, Jo-Ting; Chiang, Yung-Hsiao; Shih, Raymond; Chiu, Pei Ling; Hung, Kuo-Sheng; Yeh, Yi Shian; Wei, Li; Chiu, Wen-Ta; Yang, Liang-Yo.

In: Surgical Neurology, Vol. 72, No. SUPPL. 2, 12.2009, p. S55-S65.

Research output: Contribution to journalArticle

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title = "Biochemical alteration in cerebrospinal fluid precedes behavioral deficits in Parkinsonian rats induced by 6-hydroxydopamine",
abstract = "Background: Parkinson's disease, affecting at least 1{\%} of population older than 65 years, is the most common neurodegenerative movement disorder. Up to now, no evidence has demonstrated that biochemical changes in CSF occur preceding the onset of Parkinson's symptoms. In this study, we tested the hypothesis that biochemical changes in CSF precede behavioral deficits in Parkinsonian animals. Methods: We infused different doses of 6-OHDA into the MFB of rats bilaterally and examined the animals' movement behaviors, biochemical alterations in CSF, and dopaminergic neuronal number in the SNpc 1 week later. Results: Our results indicated that animals with over 70{\%} dopaminergic neuronal loss in the SNpc exhibited behavioral bradykinesia and rigidity, and a decrease of HVA in CSF. In contrast, animals with about 42{\%} dopaminergic neuronal loss in the SNpc showed normal movement behaviors, but displayed a drastic decline of HVA in CSF. Furthermore, the number of dopaminergic neurons in the SNpc was positively correlated with the HVA level in CSF. Conclusions: Our findings demonstrate that biochemical alteration in CSF foreruns behavioral deficits and the HVA level in CSF is positively correlated with the number of dopaminergic neurons in the SNpc of Parkinsonian rats induced by 6-OHDA. Our results strongly suggest that additional studies are needed to evaluate usefulness of monitoring the HVA level in CSF for early detection of the loss of dopaminergic neurons in the SNpc that precedes the onset of Parkinsonian symptoms in humans.",
keywords = "6-Hydroxydopamine, CSF, Parkinson's disease, Parkinsonism, Substantia nigra, Tyrosine hydroxylase",
author = "Jia-Wei Lin and Chwen-Ming Shih and Yen-Chou Chen and Chien-Min Lin and Jo-Ting Tsai and Yung-Hsiao Chiang and Raymond Shih and Chiu, {Pei Ling} and Kuo-Sheng Hung and Yeh, {Yi Shian} and Li Wei and Wen-Ta Chiu and Liang-Yo Yang",
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T1 - Biochemical alteration in cerebrospinal fluid precedes behavioral deficits in Parkinsonian rats induced by 6-hydroxydopamine

AU - Lin, Jia-Wei

AU - Shih, Chwen-Ming

AU - Chen, Yen-Chou

AU - Lin, Chien-Min

AU - Tsai, Jo-Ting

AU - Chiang, Yung-Hsiao

AU - Shih, Raymond

AU - Chiu, Pei Ling

AU - Hung, Kuo-Sheng

AU - Yeh, Yi Shian

AU - Wei, Li

AU - Chiu, Wen-Ta

AU - Yang, Liang-Yo

PY - 2009/12

Y1 - 2009/12

N2 - Background: Parkinson's disease, affecting at least 1% of population older than 65 years, is the most common neurodegenerative movement disorder. Up to now, no evidence has demonstrated that biochemical changes in CSF occur preceding the onset of Parkinson's symptoms. In this study, we tested the hypothesis that biochemical changes in CSF precede behavioral deficits in Parkinsonian animals. Methods: We infused different doses of 6-OHDA into the MFB of rats bilaterally and examined the animals' movement behaviors, biochemical alterations in CSF, and dopaminergic neuronal number in the SNpc 1 week later. Results: Our results indicated that animals with over 70% dopaminergic neuronal loss in the SNpc exhibited behavioral bradykinesia and rigidity, and a decrease of HVA in CSF. In contrast, animals with about 42% dopaminergic neuronal loss in the SNpc showed normal movement behaviors, but displayed a drastic decline of HVA in CSF. Furthermore, the number of dopaminergic neurons in the SNpc was positively correlated with the HVA level in CSF. Conclusions: Our findings demonstrate that biochemical alteration in CSF foreruns behavioral deficits and the HVA level in CSF is positively correlated with the number of dopaminergic neurons in the SNpc of Parkinsonian rats induced by 6-OHDA. Our results strongly suggest that additional studies are needed to evaluate usefulness of monitoring the HVA level in CSF for early detection of the loss of dopaminergic neurons in the SNpc that precedes the onset of Parkinsonian symptoms in humans.

AB - Background: Parkinson's disease, affecting at least 1% of population older than 65 years, is the most common neurodegenerative movement disorder. Up to now, no evidence has demonstrated that biochemical changes in CSF occur preceding the onset of Parkinson's symptoms. In this study, we tested the hypothesis that biochemical changes in CSF precede behavioral deficits in Parkinsonian animals. Methods: We infused different doses of 6-OHDA into the MFB of rats bilaterally and examined the animals' movement behaviors, biochemical alterations in CSF, and dopaminergic neuronal number in the SNpc 1 week later. Results: Our results indicated that animals with over 70% dopaminergic neuronal loss in the SNpc exhibited behavioral bradykinesia and rigidity, and a decrease of HVA in CSF. In contrast, animals with about 42% dopaminergic neuronal loss in the SNpc showed normal movement behaviors, but displayed a drastic decline of HVA in CSF. Furthermore, the number of dopaminergic neurons in the SNpc was positively correlated with the HVA level in CSF. Conclusions: Our findings demonstrate that biochemical alteration in CSF foreruns behavioral deficits and the HVA level in CSF is positively correlated with the number of dopaminergic neurons in the SNpc of Parkinsonian rats induced by 6-OHDA. Our results strongly suggest that additional studies are needed to evaluate usefulness of monitoring the HVA level in CSF for early detection of the loss of dopaminergic neurons in the SNpc that precedes the onset of Parkinsonian symptoms in humans.

KW - 6-Hydroxydopamine

KW - CSF

KW - Parkinson's disease

KW - Parkinsonism

KW - Substantia nigra

KW - Tyrosine hydroxylase

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DO - 10.1016/j.wneu.2009.09.005

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