Biochanin A, a phytoestrogenic isoflavone with selective inhibition of phosphodiesterase 4, suppresses ovalbumin-induced airway hyperresponsiveness

Chung Hung Shih, Wun-Chang Ko, Ling Hong Lin, Hsin Yi Shen, Chi Yin Lai, Chien Ming Chen

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Abstract

The present study investigated the potential of biochanin A, a phytoestrogenic isoflavone of red clover (Triflolium pratense), for use in treating asthma or chronic obstructive pulmonary disease (COPD). Biochanin A (100mol/kg, orally (p.o.)) significantly attenuated airway resistance (R L), enhanced pause (Penh), and increased lung dynamic compliance (Cdyn) values induced by methacholine (MCh) in sensitized and challenged mice. It also significantly suppressed an increase in the number of total inflammatory cells, neutrophils, and eosinophils, and levels of cytokines, including interleukin (IL)-2, IL-4, IL-5, and tumor necrosis factor (TNF)- in bronchoalveolar lavage fluid (BALF) of the mice. However, it did not influence interferon (IFN)- levels. Biochanin A (100μmol/kg, p.o.) also significantly suppressed the total and ovalbumin (OVA)-specific immunoglobulin E (IgE) levels in the serum and BALF, and enhanced the total IgG2a level in the serum of these mice. The PDE4H/PDE4L value of biochanin A was calculated as >35. Biochanin A did not influence xylazine/ketamine-induced anesthesia. Biochanin A (10-30μM) significantly reduced cumulative OVA (10~100g/mL)-induced contractions in the isolated guinea pig trachealis, suggesting that it inhibits degranulation of mast cells. In conclusion, red clover containing biochanin A has the potential for treating allergic asthma and COPD.

Original languageEnglish
Article number635058
JournalEvidence-based Complementary and Alternative Medicine
Volume2011
DOIs
Publication statusPublished - 2011

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Type 4 Cyclic Nucleotide Phosphodiesterase
Isoflavones
Ovalbumin
Trifolium
Bronchoalveolar Lavage Fluid
Chronic Obstructive Pulmonary Disease
Asthma
Xylazine
Lung Compliance
Airway Resistance
Methacholine Chloride
Interleukin-5
Ketamine
biochanin A
Serum
Eosinophils
Mast Cells
Interleukin-4
Interferons
Immunoglobulin E

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Biochanin A, a phytoestrogenic isoflavone with selective inhibition of phosphodiesterase 4, suppresses ovalbumin-induced airway hyperresponsiveness. / Shih, Chung Hung; Ko, Wun-Chang; Lin, Ling Hong; Shen, Hsin Yi; Lai, Chi Yin; Chen, Chien Ming.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2011, 635058, 2011.

Research output: Contribution to journalArticle

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