Bioactive peptides attenuate cardiac apoptosis in spontaneously hypertensive rat hearts through activation of autophagy and mitochondrial biogenesis pathway

Wan Teng Lin, Srinivasan Nithiyanantham, Dennis Jine Yuan Hsieh, Ray Jade Chen, Cecilia Hsuan Day, Jia Ying Liao, Chia Hua Kuo, B. Mahalakshmi, Wei Wen Kuo, Chih Yang Huang

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Alcalase potato protein hydrolysate (APPH) might have a very important role in therapeutic effects. This study aims to examine the beneficial effects of bioactive peptides (DIKTNKPVIF [DI] and IF) from APPH supplement in the regulation of cardiac apoptosis, autophagy, and mitochondrial biogenesis pathway in spontaneously hypertensive rats (SHR). We have investigated ejection fraction, fractional shortening, Tunel assay, apoptosis, autophagy, and mitochondrial biogenesis pathway marker expression to show the efficacy of bioactive peptides in an SHR model. Bioactive peptides significantly upregulate ejection fraction and fractional shortening in SHR rats. SHR rats exhibited higher protein expression of apoptotic markers such as BAD, cytochrome c, and caspase 3. Finally, the bioactive peptides upregulate survival proteins (p-AKT/p-PI3K), autophagy (Beclin1/LC3B), and mitochondrial biogenesis (p-AMPKα/SIRT1/PGC1α/p-Foxo3a/Nrf2/CREB) marker expressions compared with the SHR groups. In summary, the bioactive peptides protect the heart tissues through the activation of autophagy and mitochondrial biogenesis pathway and thereby attenuate cardiac apoptosis in a spontaneously hypertensive rat model.

Original languageEnglish
Pages (from-to)804-810
Number of pages7
JournalEnvironmental Toxicology
Volume35
Issue number7
DOIs
Publication statusPublished - Jul 1 2020

Keywords

  • apoptosis
  • autophagy
  • bioactive peptides
  • mitochondrial biogenesis
  • SHR

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

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