Binding and Partial Denaturing of G-quartet DNA by Cdc13p of Saccharomyces cerevisiae

Yi Chien Lin, Jing Wen Shih, Chia Ling Hsu, Jing Jer Lin

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The protein Cdc13p binds telomeres in vivo and is essential for the maintenance of the telomeres of Saccharomyces cerevisiae. In addition, Cdc13p is known to bind single-stranded TG1-3 DNA in vitro. Here we have shown that Cdc13p also binds DNA quadruplex, G-quartet, formed by TG 1-3 DNA. Moreover, the binding of Cde13p causes a partial denaturing of the G-quartet DNA. Formation of DNA quadruplexes may involve the intermolecular association of TG1-3 DNA and inhibit the extension of telomeres by telomerase. Thus, our finding suggests that Cde13p may disrupt telomere association and facilitate telomere replication.

Original languageEnglish
Pages (from-to)47671-47674
Number of pages4
JournalJournal of Biological Chemistry
Volume276
Issue number50
DOIs
Publication statusPublished - Dec 14 2001
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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