TY - JOUR
T1 - Binding and Partial Denaturing of G-quartet DNA by Cdc13p of Saccharomyces cerevisiae
AU - Lin, Yi Chien
AU - Shih, Jing Wen
AU - Hsu, Chia Ling
AU - Lin, Jing Jer
PY - 2001/12/14
Y1 - 2001/12/14
N2 - The protein Cdc13p binds telomeres in vivo and is essential for the maintenance of the telomeres of Saccharomyces cerevisiae. In addition, Cdc13p is known to bind single-stranded TG1-3 DNA in vitro. Here we have shown that Cdc13p also binds DNA quadruplex, G-quartet, formed by TG 1-3 DNA. Moreover, the binding of Cde13p causes a partial denaturing of the G-quartet DNA. Formation of DNA quadruplexes may involve the intermolecular association of TG1-3 DNA and inhibit the extension of telomeres by telomerase. Thus, our finding suggests that Cde13p may disrupt telomere association and facilitate telomere replication.
AB - The protein Cdc13p binds telomeres in vivo and is essential for the maintenance of the telomeres of Saccharomyces cerevisiae. In addition, Cdc13p is known to bind single-stranded TG1-3 DNA in vitro. Here we have shown that Cdc13p also binds DNA quadruplex, G-quartet, formed by TG 1-3 DNA. Moreover, the binding of Cde13p causes a partial denaturing of the G-quartet DNA. Formation of DNA quadruplexes may involve the intermolecular association of TG1-3 DNA and inhibit the extension of telomeres by telomerase. Thus, our finding suggests that Cde13p may disrupt telomere association and facilitate telomere replication.
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U2 - 10.1074/jbc.M104989200
DO - 10.1074/jbc.M104989200
M3 - Article
C2 - 11585819
AN - SCOPUS:0035861591
SN - 0021-9258
VL - 276
SP - 47671
EP - 47674
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 50
ER -