Binary-blend fibber-based capture assay of circulating tumor cells for clinical diagnosis of colorectal cancer

Ai Wei Lee, Fu Xiang Lin, Po Li Wei, Guo Jian-Wei, Jem Kun Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: In addition to conventional approaches, detecting and characterizing CTCs in patient blood allows for early diagnosis of cancer metastasis. Methods: We blended poly(ethylene oxide) (PEO) into nylon-6 through electrospinning to generate a fibrous matbased circulating tumour cells (CTCs) assay. The contents of nylon-6 and PEO in the electrospun blend fibrous mats (EBFMs) were optimized to facilitate high cell-substrate affinity and low leukocyte adsorption. Results: Compared with the IsoFlux System, a commercial instrument for CTC detection, the CTC assay of EBFMs exhibited lower false positive readings and high sensitivity and selectivity with preclinical specimens. Furthermore, we examined the clinical diagnosis accuracy of colorectal cancer, using the CTC assay and compared the results with those identified through pathological analyses of biopsies from colonoscopies. Our positive expressions of colorectal cancer through CTC detection completely matched those recognized through the pathological analyses for the individuals having stage II, III, and IV colorectal cancer. Nevertheless, two in four individuals having stage I colorectal cancer, recognized through pathological analysis of biopsies from colonoscopies, exhibited positive expression of CTCs. Ten individuals were identified through pathological analysis as having no colorectal tumours. Nevertheless, two of these ten individuals exhibited positive expression of CTCs. Conclusions: Thus, in this population, the low cost EBFMs exhibited considerable capture efficiency for the non-invasive diagnosis of colorectal cancer.

Original languageEnglish
Article number4
JournalJournal of Nanobiotechnology
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 16 2018

Fingerprint

Circulating Neoplastic Cells
Tumors
Colorectal Neoplasms
Assays
Cells
Polyethylene oxides
Colonoscopy
Biopsy
Ethylene Oxide
Early Detection of Cancer
Electrospinning
Adsorption
Reading
Leukocytes
Blood
Neoplasm Metastasis
Costs and Cost Analysis

Keywords

  • Circulating tumour cell
  • Colorectal cancer diagnosis
  • Electrospinning
  • Nylon-6

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Pharmaceutical Science

Cite this

Binary-blend fibber-based capture assay of circulating tumor cells for clinical diagnosis of colorectal cancer. / Lee, Ai Wei; Lin, Fu Xiang; Wei, Po Li; Jian-Wei, Guo; Chen, Jem Kun.

In: Journal of Nanobiotechnology, Vol. 16, No. 1, 4, 16.01.2018.

Research output: Contribution to journalArticle

@article{33971e122b694987967d7474ea1c5319,
title = "Binary-blend fibber-based capture assay of circulating tumor cells for clinical diagnosis of colorectal cancer",
abstract = "Background: In addition to conventional approaches, detecting and characterizing CTCs in patient blood allows for early diagnosis of cancer metastasis. Methods: We blended poly(ethylene oxide) (PEO) into nylon-6 through electrospinning to generate a fibrous matbased circulating tumour cells (CTCs) assay. The contents of nylon-6 and PEO in the electrospun blend fibrous mats (EBFMs) were optimized to facilitate high cell-substrate affinity and low leukocyte adsorption. Results: Compared with the IsoFlux System, a commercial instrument for CTC detection, the CTC assay of EBFMs exhibited lower false positive readings and high sensitivity and selectivity with preclinical specimens. Furthermore, we examined the clinical diagnosis accuracy of colorectal cancer, using the CTC assay and compared the results with those identified through pathological analyses of biopsies from colonoscopies. Our positive expressions of colorectal cancer through CTC detection completely matched those recognized through the pathological analyses for the individuals having stage II, III, and IV colorectal cancer. Nevertheless, two in four individuals having stage I colorectal cancer, recognized through pathological analysis of biopsies from colonoscopies, exhibited positive expression of CTCs. Ten individuals were identified through pathological analysis as having no colorectal tumours. Nevertheless, two of these ten individuals exhibited positive expression of CTCs. Conclusions: Thus, in this population, the low cost EBFMs exhibited considerable capture efficiency for the non-invasive diagnosis of colorectal cancer.",
keywords = "Circulating tumour cell, Colorectal cancer diagnosis, Electrospinning, Nylon-6",
author = "Lee, {Ai Wei} and Lin, {Fu Xiang} and Wei, {Po Li} and Guo Jian-Wei and Chen, {Jem Kun}",
year = "2018",
month = "1",
day = "16",
doi = "10.1186/s12951-017-0330-1",
language = "English",
volume = "16",
journal = "Journal of Nanobiotechnology",
issn = "1477-3155",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Binary-blend fibber-based capture assay of circulating tumor cells for clinical diagnosis of colorectal cancer

AU - Lee, Ai Wei

AU - Lin, Fu Xiang

AU - Wei, Po Li

AU - Jian-Wei, Guo

AU - Chen, Jem Kun

PY - 2018/1/16

Y1 - 2018/1/16

N2 - Background: In addition to conventional approaches, detecting and characterizing CTCs in patient blood allows for early diagnosis of cancer metastasis. Methods: We blended poly(ethylene oxide) (PEO) into nylon-6 through electrospinning to generate a fibrous matbased circulating tumour cells (CTCs) assay. The contents of nylon-6 and PEO in the electrospun blend fibrous mats (EBFMs) were optimized to facilitate high cell-substrate affinity and low leukocyte adsorption. Results: Compared with the IsoFlux System, a commercial instrument for CTC detection, the CTC assay of EBFMs exhibited lower false positive readings and high sensitivity and selectivity with preclinical specimens. Furthermore, we examined the clinical diagnosis accuracy of colorectal cancer, using the CTC assay and compared the results with those identified through pathological analyses of biopsies from colonoscopies. Our positive expressions of colorectal cancer through CTC detection completely matched those recognized through the pathological analyses for the individuals having stage II, III, and IV colorectal cancer. Nevertheless, two in four individuals having stage I colorectal cancer, recognized through pathological analysis of biopsies from colonoscopies, exhibited positive expression of CTCs. Ten individuals were identified through pathological analysis as having no colorectal tumours. Nevertheless, two of these ten individuals exhibited positive expression of CTCs. Conclusions: Thus, in this population, the low cost EBFMs exhibited considerable capture efficiency for the non-invasive diagnosis of colorectal cancer.

AB - Background: In addition to conventional approaches, detecting and characterizing CTCs in patient blood allows for early diagnosis of cancer metastasis. Methods: We blended poly(ethylene oxide) (PEO) into nylon-6 through electrospinning to generate a fibrous matbased circulating tumour cells (CTCs) assay. The contents of nylon-6 and PEO in the electrospun blend fibrous mats (EBFMs) were optimized to facilitate high cell-substrate affinity and low leukocyte adsorption. Results: Compared with the IsoFlux System, a commercial instrument for CTC detection, the CTC assay of EBFMs exhibited lower false positive readings and high sensitivity and selectivity with preclinical specimens. Furthermore, we examined the clinical diagnosis accuracy of colorectal cancer, using the CTC assay and compared the results with those identified through pathological analyses of biopsies from colonoscopies. Our positive expressions of colorectal cancer through CTC detection completely matched those recognized through the pathological analyses for the individuals having stage II, III, and IV colorectal cancer. Nevertheless, two in four individuals having stage I colorectal cancer, recognized through pathological analysis of biopsies from colonoscopies, exhibited positive expression of CTCs. Ten individuals were identified through pathological analysis as having no colorectal tumours. Nevertheless, two of these ten individuals exhibited positive expression of CTCs. Conclusions: Thus, in this population, the low cost EBFMs exhibited considerable capture efficiency for the non-invasive diagnosis of colorectal cancer.

KW - Circulating tumour cell

KW - Colorectal cancer diagnosis

KW - Electrospinning

KW - Nylon-6

UR - http://www.scopus.com/inward/record.url?scp=85040704250&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85040704250&partnerID=8YFLogxK

U2 - 10.1186/s12951-017-0330-1

DO - 10.1186/s12951-017-0330-1

M3 - Article

AN - SCOPUS:85040704250

VL - 16

JO - Journal of Nanobiotechnology

JF - Journal of Nanobiotechnology

SN - 1477-3155

IS - 1

M1 - 4

ER -