Betel nut extract and arecoline block insulin signaling and lipid storage in 3T3-L1 adipocytes

Tusty Jiuan Hsieh, Pei Chen Hsieh, Ming Tsang Wu, Wei Chiao Chang, Pi Jung Hsiao, Kun Der Lin, Pong Chun Chou, Shyi Jang Shin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

According to several population-based studies, betel nut chewing is associated with metabolic syndrome and diabetes in British South Asians and Taiwanese. However, the underlying molecular mechanism is not yet clear. Arecoline is an alkaloid-type natural product found in betel nuts. Our aim was to clarify the influence of betel nut extract and arecoline on lipid accumulation and insulin signaling in adipocytes. We found that betel nut extract and arecoline blocked lipid storage in 3T3-L1 adipocytes. The possible mechanism may function by inhibiting the expression of the insulin receptor, glucose transporter-4, fatty acid synthase, and the lipid droplet proteins perilipin and adipophilin. In addition, betel nut extract and arecoline increased the basal level of IRS-1 serine 307 phosphorylation and decreased insulin-stimulated IRS-1 tyrosine, Akt, and PI3 kinase phosphorylation. In conclusion, betel nut extract and arecoline have diabetogenic potential on adipocytes that may result in insulin resistance and diabetes at least in part via the obstruction of insulin signaling and the blockage of lipid storage.

Original languageEnglish
Pages (from-to)397-411
Number of pages15
JournalCell Biology and Toxicology
Volume27
Issue number6
DOIs
Publication statusPublished - Dec 2011
Externally publishedYes

Fingerprint

Arecoline
Areca
Adipocytes
Insulin
Lipids
Phosphorylation
Medical problems
Mastication
Fatty Acid Synthases
Facilitative Glucose Transport Proteins
Insulin Receptor
Biological Products
Phosphatidylinositol 3-Kinases
Alkaloids
Protein-Tyrosine Kinases
Serine
Tyrosine
Insulin Resistance
Population

Keywords

  • Betel nut
  • Diabetes
  • Insulin resistance
  • IRS-1
  • Perilipin

ASJC Scopus subject areas

  • Cell Biology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Betel nut extract and arecoline block insulin signaling and lipid storage in 3T3-L1 adipocytes. / Hsieh, Tusty Jiuan; Hsieh, Pei Chen; Wu, Ming Tsang; Chang, Wei Chiao; Hsiao, Pi Jung; Lin, Kun Der; Chou, Pong Chun; Shin, Shyi Jang.

In: Cell Biology and Toxicology, Vol. 27, No. 6, 12.2011, p. 397-411.

Research output: Contribution to journalArticle

Hsieh, Tusty Jiuan ; Hsieh, Pei Chen ; Wu, Ming Tsang ; Chang, Wei Chiao ; Hsiao, Pi Jung ; Lin, Kun Der ; Chou, Pong Chun ; Shin, Shyi Jang. / Betel nut extract and arecoline block insulin signaling and lipid storage in 3T3-L1 adipocytes. In: Cell Biology and Toxicology. 2011 ; Vol. 27, No. 6. pp. 397-411.
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