Beneficial effect of astragalosides on stroke condition using PC12 cells under oxygen glucose deprivation and reperfusion

Bi Ying Chiu, Ching Ping Chang, Jia Wei Lin, Jung Sheng Yu, Wen Pin Liu, Yao Chin Hsu, Mao Tsun Lin

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Astragalosides (AST) are reported to be neuroprotective in focal cerebral ischemic models in vivo. In this study, the direct effect of AST against oxygen and glucose deprivation (OGD) including neuronal injury and the underlying mechanisms in vitro were investigated. 5 h OGD followed by 24 h of reperfusion [adding back oxygen and glucose (OGD-R)] was used to induce in vitro ischemia reperfusion injury in differentiated rat pheochromocytoma PC12 cells. AST (1, 100, and 200 μg/mL) were added to the culture after 5 h of the OGD ischemic insult and was present during the reoxygenation phases. A key finding was that OGD-R decreased cell viability, increased lactate dehydrogenase, increased reactive oxygen species, apoptosis, autophagy, functional impairment of mitochondria, and endoplasmic reticulum stress in PC12 cells, all of which AST treatment significantly reduced. In addition, AST attenuated OGD-R-induced cell loss through P38 MAPK activation a neuroprotective effect blunted by SB203580, a specific inhibitor of P38 MAPK. Our data suggest that both apoptosis and autophagy are important characteristics of OGD-R-induced PC12 death and that treating PC12 cells with AST blocked OGD-R-induced apoptosis and autophagy by suppressing intracellular oxidative stress, functional impairment of mitochondria, and endoplasmic reticulum stress. Our data provide identification of AST that can concomitantly inhibit multiple cells death pathways following OGD injuries in neural cells.

Original languageEnglish
Pages (from-to)825-837
Number of pages13
JournalCellular and Molecular Neurobiology
Volume34
Issue number6
DOIs
Publication statusPublished - 2014

Keywords

  • Apoptosis
  • Astragaloside
  • Autophagy
  • Mitochondria
  • Oxygen glucose deprivation
  • Reactive oxygen species

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Medicine(all)

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