Basic fibroblast growth factor induces VEGF expression in chondrosarcoma cells and subsequently promotes endothelial progenitor cell-primed angiogenesis

Huey En Tzeng, Po Chun Chen, Kai Wei Lin, Chih Yang Lin, Chun Hao Tsai, Shao Min Han, Chieh Lin Teng, Wen Li Hwang, Shih Wei Wang, Chih Hsin Tang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Chondrosarcoma, a common malignant tumour, develops in bone. Effective adjuvant therapy remains inadequate for treatment, meaning poor prognosis. It is imperative to explore novel remedies. Angiogenesis is a rate-limiting step in progression that explains neovessel formation for blood supply in the tumour microenvironment. Numerous studies indicate that EPCs (endothelial progenitor cells) promote angiogenesis and contribute to tumour growth. bFGF (basic fibroblast growth factor), a secreted cytokine, regulates biological activity, including angiogenesis, and correlates with tumorigenesis. However, the role of bFGF in angiogenesis-related tumour progression by recruiting EPCs in human chondrosarcoma is rarely discussed. In the present study, we found that bFGF induced VEGF (vascular endothelial growth factor) expression via the FGFR1 (fibroblast growth factor receptor 1)/c-Src/p38/NF-κB (nuclear factor κB) signalling pathway in chondrosarcoma cells, thereby triggering angiogenesis of endothelial progenitor cells. Our in vivo data revealed that tumour-secreted bFGF promotes angiogenesis in both mouse plug and chick CAM (chorioallantoic membrane) assays. Xenograft mouse model data, due to bFGF-regulated angiogenesis, showed the bFGF regulates angiogenesis-linked tumour growth. Finally, bFGF was highly expressed in chondrosarcoma patients compared with normal cartilage, positively correlating with VEGF expression and tumour stage. The present study reveals a novel therapeutic target for chondrosarcoma progression.

Original languageEnglish
Pages (from-to)147-158
Number of pages12
JournalClinical Science
Volume129
Issue number2
DOIs
Publication statusPublished - Jan 1 2015
Externally publishedYes

Fingerprint

Chondrosarcoma
Fibroblast Growth Factor 2
Vascular Endothelial Growth Factor A
Neoplasms
Receptor, Fibroblast Growth Factor, Type 1
Chorioallantoic Membrane
Tumor Microenvironment
Growth
Endothelial Progenitor Cells
Heterografts
Cartilage
Carcinogenesis
Therapeutics
Cytokines
Bone and Bones

Keywords

  • Angiogenesis
  • Basic fibroblast growth factor
  • Chondrosarcoma
  • Endothelial progenitor cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Basic fibroblast growth factor induces VEGF expression in chondrosarcoma cells and subsequently promotes endothelial progenitor cell-primed angiogenesis. / Tzeng, Huey En; Chen, Po Chun; Lin, Kai Wei; Lin, Chih Yang; Tsai, Chun Hao; Han, Shao Min; Teng, Chieh Lin; Hwang, Wen Li; Wang, Shih Wei; Tang, Chih Hsin.

In: Clinical Science, Vol. 129, No. 2, 01.01.2015, p. 147-158.

Research output: Contribution to journalArticle

Tzeng, Huey En ; Chen, Po Chun ; Lin, Kai Wei ; Lin, Chih Yang ; Tsai, Chun Hao ; Han, Shao Min ; Teng, Chieh Lin ; Hwang, Wen Li ; Wang, Shih Wei ; Tang, Chih Hsin. / Basic fibroblast growth factor induces VEGF expression in chondrosarcoma cells and subsequently promotes endothelial progenitor cell-primed angiogenesis. In: Clinical Science. 2015 ; Vol. 129, No. 2. pp. 147-158.
@article{0cfaeb3cf8764954aa8b451f6f7314a6,
title = "Basic fibroblast growth factor induces VEGF expression in chondrosarcoma cells and subsequently promotes endothelial progenitor cell-primed angiogenesis",
abstract = "Chondrosarcoma, a common malignant tumour, develops in bone. Effective adjuvant therapy remains inadequate for treatment, meaning poor prognosis. It is imperative to explore novel remedies. Angiogenesis is a rate-limiting step in progression that explains neovessel formation for blood supply in the tumour microenvironment. Numerous studies indicate that EPCs (endothelial progenitor cells) promote angiogenesis and contribute to tumour growth. bFGF (basic fibroblast growth factor), a secreted cytokine, regulates biological activity, including angiogenesis, and correlates with tumorigenesis. However, the role of bFGF in angiogenesis-related tumour progression by recruiting EPCs in human chondrosarcoma is rarely discussed. In the present study, we found that bFGF induced VEGF (vascular endothelial growth factor) expression via the FGFR1 (fibroblast growth factor receptor 1)/c-Src/p38/NF-κB (nuclear factor κB) signalling pathway in chondrosarcoma cells, thereby triggering angiogenesis of endothelial progenitor cells. Our in vivo data revealed that tumour-secreted bFGF promotes angiogenesis in both mouse plug and chick CAM (chorioallantoic membrane) assays. Xenograft mouse model data, due to bFGF-regulated angiogenesis, showed the bFGF regulates angiogenesis-linked tumour growth. Finally, bFGF was highly expressed in chondrosarcoma patients compared with normal cartilage, positively correlating with VEGF expression and tumour stage. The present study reveals a novel therapeutic target for chondrosarcoma progression.",
keywords = "Angiogenesis, Basic fibroblast growth factor, Chondrosarcoma, Endothelial progenitor cells",
author = "Tzeng, {Huey En} and Chen, {Po Chun} and Lin, {Kai Wei} and Lin, {Chih Yang} and Tsai, {Chun Hao} and Han, {Shao Min} and Teng, {Chieh Lin} and Hwang, {Wen Li} and Wang, {Shih Wei} and Tang, {Chih Hsin}",
year = "2015",
month = "1",
day = "1",
doi = "10.1042/CS20140390",
language = "English",
volume = "129",
pages = "147--158",
journal = "Clinical Science",
issn = "0143-5221",
publisher = "Portland Press Ltd.",
number = "2",

}

TY - JOUR

T1 - Basic fibroblast growth factor induces VEGF expression in chondrosarcoma cells and subsequently promotes endothelial progenitor cell-primed angiogenesis

AU - Tzeng, Huey En

AU - Chen, Po Chun

AU - Lin, Kai Wei

AU - Lin, Chih Yang

AU - Tsai, Chun Hao

AU - Han, Shao Min

AU - Teng, Chieh Lin

AU - Hwang, Wen Li

AU - Wang, Shih Wei

AU - Tang, Chih Hsin

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Chondrosarcoma, a common malignant tumour, develops in bone. Effective adjuvant therapy remains inadequate for treatment, meaning poor prognosis. It is imperative to explore novel remedies. Angiogenesis is a rate-limiting step in progression that explains neovessel formation for blood supply in the tumour microenvironment. Numerous studies indicate that EPCs (endothelial progenitor cells) promote angiogenesis and contribute to tumour growth. bFGF (basic fibroblast growth factor), a secreted cytokine, regulates biological activity, including angiogenesis, and correlates with tumorigenesis. However, the role of bFGF in angiogenesis-related tumour progression by recruiting EPCs in human chondrosarcoma is rarely discussed. In the present study, we found that bFGF induced VEGF (vascular endothelial growth factor) expression via the FGFR1 (fibroblast growth factor receptor 1)/c-Src/p38/NF-κB (nuclear factor κB) signalling pathway in chondrosarcoma cells, thereby triggering angiogenesis of endothelial progenitor cells. Our in vivo data revealed that tumour-secreted bFGF promotes angiogenesis in both mouse plug and chick CAM (chorioallantoic membrane) assays. Xenograft mouse model data, due to bFGF-regulated angiogenesis, showed the bFGF regulates angiogenesis-linked tumour growth. Finally, bFGF was highly expressed in chondrosarcoma patients compared with normal cartilage, positively correlating with VEGF expression and tumour stage. The present study reveals a novel therapeutic target for chondrosarcoma progression.

AB - Chondrosarcoma, a common malignant tumour, develops in bone. Effective adjuvant therapy remains inadequate for treatment, meaning poor prognosis. It is imperative to explore novel remedies. Angiogenesis is a rate-limiting step in progression that explains neovessel formation for blood supply in the tumour microenvironment. Numerous studies indicate that EPCs (endothelial progenitor cells) promote angiogenesis and contribute to tumour growth. bFGF (basic fibroblast growth factor), a secreted cytokine, regulates biological activity, including angiogenesis, and correlates with tumorigenesis. However, the role of bFGF in angiogenesis-related tumour progression by recruiting EPCs in human chondrosarcoma is rarely discussed. In the present study, we found that bFGF induced VEGF (vascular endothelial growth factor) expression via the FGFR1 (fibroblast growth factor receptor 1)/c-Src/p38/NF-κB (nuclear factor κB) signalling pathway in chondrosarcoma cells, thereby triggering angiogenesis of endothelial progenitor cells. Our in vivo data revealed that tumour-secreted bFGF promotes angiogenesis in both mouse plug and chick CAM (chorioallantoic membrane) assays. Xenograft mouse model data, due to bFGF-regulated angiogenesis, showed the bFGF regulates angiogenesis-linked tumour growth. Finally, bFGF was highly expressed in chondrosarcoma patients compared with normal cartilage, positively correlating with VEGF expression and tumour stage. The present study reveals a novel therapeutic target for chondrosarcoma progression.

KW - Angiogenesis

KW - Basic fibroblast growth factor

KW - Chondrosarcoma

KW - Endothelial progenitor cells

UR - http://www.scopus.com/inward/record.url?scp=84935434212&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84935434212&partnerID=8YFLogxK

U2 - 10.1042/CS20140390

DO - 10.1042/CS20140390

M3 - Article

VL - 129

SP - 147

EP - 158

JO - Clinical Science

JF - Clinical Science

SN - 0143-5221

IS - 2

ER -