Baicalin Induces Differential Expression of Cytochrome C Oxidase in Human Lung H441 Cell

Kur T. Cheng, Wen C. Hou, Yu Chen Huang, Leng-Fang Wang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In a previous study, we evaluated the effect of baicalin on the expression of SP-A (surfactant protein A), which was developmentally regulated in an alveolar type II cell, H441. SP-A is encoded by two similar genes, SP-A1 and SP-A2, in humans. The maximal induction of SP-A1 gene of H441 occurred at treating 150 nM of baicalin for 48 h. In the present study, cDNA subtraction analysis is performed to examine the differential expression in H441 cell upon baicalin treatment with a view to investigating the regulatory mechanism. The mRNA of H441 cell incubated with 150 nM baicalin for 48 h was compared to that of blank control. Two PCR products were obtained through subtractive cDNA amplification. A product encoding cytochrome c oxidase was demonstrated to be a differential signal by RT-PCR analysis, and the other was a false positive. The induction of cytochrome c oxidase might increase ATP level in cell, and consequently elevates cAMP, which upregulates surfactant synthesis and secretion.

Original languageEnglish
Pages (from-to)7276-7279
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number25
DOIs
Publication statusPublished - Dec 3 2003

Fingerprint

Electron Transport Complex IV
Cytochromes
cytochromes
Oxidoreductases
lungs
surfactants
Pulmonary Surfactant-Associated Protein A
Lung
cytochrome-c oxidase
Complementary DNA
Genes
cells
Alveolar Epithelial Cells
Polymerase Chain Reaction
varespladib methyl
Surface-Active Agents
Amplification
Up-Regulation
genes
proteins

Keywords

  • Baicalin
  • CDNA subtraction
  • Cytochrome c oxidase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Baicalin Induces Differential Expression of Cytochrome C Oxidase in Human Lung H441 Cell. / Cheng, Kur T.; Hou, Wen C.; Huang, Yu Chen; Wang, Leng-Fang.

In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 25, 03.12.2003, p. 7276-7279.

Research output: Contribution to journalArticle

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N2 - In a previous study, we evaluated the effect of baicalin on the expression of SP-A (surfactant protein A), which was developmentally regulated in an alveolar type II cell, H441. SP-A is encoded by two similar genes, SP-A1 and SP-A2, in humans. The maximal induction of SP-A1 gene of H441 occurred at treating 150 nM of baicalin for 48 h. In the present study, cDNA subtraction analysis is performed to examine the differential expression in H441 cell upon baicalin treatment with a view to investigating the regulatory mechanism. The mRNA of H441 cell incubated with 150 nM baicalin for 48 h was compared to that of blank control. Two PCR products were obtained through subtractive cDNA amplification. A product encoding cytochrome c oxidase was demonstrated to be a differential signal by RT-PCR analysis, and the other was a false positive. The induction of cytochrome c oxidase might increase ATP level in cell, and consequently elevates cAMP, which upregulates surfactant synthesis and secretion.

AB - In a previous study, we evaluated the effect of baicalin on the expression of SP-A (surfactant protein A), which was developmentally regulated in an alveolar type II cell, H441. SP-A is encoded by two similar genes, SP-A1 and SP-A2, in humans. The maximal induction of SP-A1 gene of H441 occurred at treating 150 nM of baicalin for 48 h. In the present study, cDNA subtraction analysis is performed to examine the differential expression in H441 cell upon baicalin treatment with a view to investigating the regulatory mechanism. The mRNA of H441 cell incubated with 150 nM baicalin for 48 h was compared to that of blank control. Two PCR products were obtained through subtractive cDNA amplification. A product encoding cytochrome c oxidase was demonstrated to be a differential signal by RT-PCR analysis, and the other was a false positive. The induction of cytochrome c oxidase might increase ATP level in cell, and consequently elevates cAMP, which upregulates surfactant synthesis and secretion.

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