Baicalein attenuates intimal hyperplasia after rat carotid balloon injury through arresting cell-cycle progression and inhibiting ERK, Akt, and NF-κB activity in vascular smooth-muscle cells

Chieh Yu Peng, Shiow Lin Pan, Ying Wen Huang, Jih Hwa Guh, Ya Ling Chang, Che Ming Teng

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Baicalein (5,6,7-trioxyflavone-7-O-beta-D-glucuronide) derived from the Chinese herb Scutellaria baicalensis is well known as a lipoxygenase inhibitor. We investigated baicalein-mediated inhibitory effects on vascular smooth-muscle cell (VSMC) proliferation and intimal hyperplasia by balloon angioplasty in the rat. In vascular injury studies, baicalein significantly suppressed intimal hyperplasia by balloon angioplasty. Baicalein significantly inhibited cell proliferation via a lipoxygenase-independent pathway using [3H] thymidine incorporation, 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT), and flow cytometry assays. At the concentrations used, no cytotoxic effect on cell culture was found. Baicalein blocks cell-cycle progression in S/G2/M phase, consistent with the cell-cycle effects, baicalein significant inhibited cyclin D1, p42/44 mitogen-activated protein kinase (MAPK), and Akt phosphorylation without change in the other cell-cycle regulatory proteins. Furthermore, baicalein attenuated serum-induced deoxyribonucleic acid (DNA) binding activity of nuclear factor kappa B (NF-κB). These results show that baicalein blocks cell proliferation via blocking cell-cycle progression and proliferating events, including p42/44 MAPK and Akt activations as well as NF-κB activation. It also inhibits intimal hyperplasia after balloon vascular injury in the rat, indicating the therapeutic potential for treating restenosis after arterial injury.

Original languageEnglish
Pages (from-to)579-588
Number of pages10
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume378
Issue number6
DOIs
Publication statusPublished - Dec 2008
Externally publishedYes

Fingerprint

Tunica Intima
NF-kappa B
Vascular Smooth Muscle
Smooth Muscle Myocytes
Hyperplasia
Cell Cycle
Wounds and Injuries
Balloon Angioplasty
Vascular System Injuries
Mitogen-Activated Protein Kinase 1
Cell Proliferation
Scutellaria baicalensis
Lipoxygenase Inhibitors
Cell Cycle Proteins
Lipoxygenase
baicalein
G2 Phase
Cyclin D1
Glucuronides
Cell Division

Keywords

  • Baicalein
  • Proliferation
  • Restenosis
  • Vascular smooth-muscle cells

ASJC Scopus subject areas

  • Pharmacology

Cite this

Baicalein attenuates intimal hyperplasia after rat carotid balloon injury through arresting cell-cycle progression and inhibiting ERK, Akt, and NF-κB activity in vascular smooth-muscle cells. / Peng, Chieh Yu; Pan, Shiow Lin; Huang, Ying Wen; Guh, Jih Hwa; Chang, Ya Ling; Teng, Che Ming.

In: Naunyn-Schmiedeberg's Archives of Pharmacology, Vol. 378, No. 6, 12.2008, p. 579-588.

Research output: Contribution to journalArticle

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