Baicalein, an active component of Scutellaria baicalensis Georgi, improves cardiac contractile function in endotoxaemic rats via induction of heme oxygenase-1 and suppression of inflammatory responses

Yen Mei Lee, Pao Yun Cheng, Lih Shin Chim, Ching Wen Kung, Shuk Man Ka, Ming Tzeung Chung, Joen Rong Sheu

Research output: Contribution to journalArticle

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Abstract

Aim of the study: To evaluate the protective effect of baicalein on myocardial dysfunction caused by endotoxaemia in rats and to explore the possible mechanisms. Materials and methods: Baicalein (10 mg/kg, intravenous) was administered to conscious Wistar rats 30 min after lipopolysaccharide (LPS; 10 mg/kg, intravenous) challenge. Six hours after LPS administration, the contractile function of the isolated heart was examined using the Langendorff technique. Cardiac protein expression related to inflammatory responses, superoxide anion production and caspase-3 activity were measured. Results: Post-treatment with baicalein significantly attenuated the LPS-induced hypotension with accompanying tachycardia. The contractile function of isolated heart was significantly preserved 6 h after LPS administration, following treatment with baicalein. Furthermore, baicalein induced the expression of heme oxygenase-1 protein and reduced superoxide anion formation in the myocardium of LPS-treated rats. Cardiac levels of inducible nitric oxide synthase, monocyte chemoattractant protein-1, phospho-IκBα and phospho-p65 protein and caspase-3 activity significantly increased 6 h after LPS challenge but baicalein significantly attenuated these LPS-induced changes. Conclusions: Baicalein improves myocardial contractility in LPS-induced sepsis, which may be related to reductions in oxidative stress, myocardial inflammatory responses and apoptosis.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalJournal of Ethnopharmacology
Volume135
Issue number1
DOIs
Publication statusPublished - Apr 26 2011

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Scutellaria baicalensis
Heme Oxygenase-1
Superoxides
Caspase 3
Controlled Hypotension
Proteins
Endotoxemia
Chemokine CCL2
Nitric Oxide Synthase Type II
baicalein
Tachycardia
Lipopolysaccharides
Wistar Rats
Sepsis
Myocardium
Oxidative Stress

Keywords

  • Apoptosis
  • Baicalein
  • Inflammation
  • Myocardial dysfunction
  • Oxidative stress
  • Sepsis

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Baicalein, an active component of Scutellaria baicalensis Georgi, improves cardiac contractile function in endotoxaemic rats via induction of heme oxygenase-1 and suppression of inflammatory responses. / Lee, Yen Mei; Cheng, Pao Yun; Chim, Lih Shin; Kung, Ching Wen; Ka, Shuk Man; Chung, Ming Tzeung; Sheu, Joen Rong.

In: Journal of Ethnopharmacology, Vol. 135, No. 1, 26.04.2011, p. 179-185.

Research output: Contribution to journalArticle

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abstract = "Aim of the study: To evaluate the protective effect of baicalein on myocardial dysfunction caused by endotoxaemia in rats and to explore the possible mechanisms. Materials and methods: Baicalein (10 mg/kg, intravenous) was administered to conscious Wistar rats 30 min after lipopolysaccharide (LPS; 10 mg/kg, intravenous) challenge. Six hours after LPS administration, the contractile function of the isolated heart was examined using the Langendorff technique. Cardiac protein expression related to inflammatory responses, superoxide anion production and caspase-3 activity were measured. Results: Post-treatment with baicalein significantly attenuated the LPS-induced hypotension with accompanying tachycardia. The contractile function of isolated heart was significantly preserved 6 h after LPS administration, following treatment with baicalein. Furthermore, baicalein induced the expression of heme oxygenase-1 protein and reduced superoxide anion formation in the myocardium of LPS-treated rats. Cardiac levels of inducible nitric oxide synthase, monocyte chemoattractant protein-1, phospho-IκBα and phospho-p65 protein and caspase-3 activity significantly increased 6 h after LPS challenge but baicalein significantly attenuated these LPS-induced changes. Conclusions: Baicalein improves myocardial contractility in LPS-induced sepsis, which may be related to reductions in oxidative stress, myocardial inflammatory responses and apoptosis.",
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AB - Aim of the study: To evaluate the protective effect of baicalein on myocardial dysfunction caused by endotoxaemia in rats and to explore the possible mechanisms. Materials and methods: Baicalein (10 mg/kg, intravenous) was administered to conscious Wistar rats 30 min after lipopolysaccharide (LPS; 10 mg/kg, intravenous) challenge. Six hours after LPS administration, the contractile function of the isolated heart was examined using the Langendorff technique. Cardiac protein expression related to inflammatory responses, superoxide anion production and caspase-3 activity were measured. Results: Post-treatment with baicalein significantly attenuated the LPS-induced hypotension with accompanying tachycardia. The contractile function of isolated heart was significantly preserved 6 h after LPS administration, following treatment with baicalein. Furthermore, baicalein induced the expression of heme oxygenase-1 protein and reduced superoxide anion formation in the myocardium of LPS-treated rats. Cardiac levels of inducible nitric oxide synthase, monocyte chemoattractant protein-1, phospho-IκBα and phospho-p65 protein and caspase-3 activity significantly increased 6 h after LPS challenge but baicalein significantly attenuated these LPS-induced changes. Conclusions: Baicalein improves myocardial contractility in LPS-induced sepsis, which may be related to reductions in oxidative stress, myocardial inflammatory responses and apoptosis.

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