BAD-lectins: Boronic acid-decorated lectins with enhanced binding affinity for the selective enrichment of glycoproteins

Ying Wei Lu, Chih Wei Chien, Po Chiao Lin, Li De Huang, Chang Yang Chen, Sz Wei Wu, Chia Li Han, Kay Hooi Khoo, Chun Cheng Lin, Yu Ju Chen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The weak and variable binding affinities exhibited by lectin-carbohydrate interactions have often compromised the practical utility of lectin in capturing glycoproteins for glycoproteomic applications. We report here the development and applications of a new type of hybrid biomaterial, namely a boronic acid-decorated lectin (BAD-lectin), for efficient bifunctional glycoprotein labeling and enrichment. Our binding studies showed an enhanced affinity by BAD-lectin, likely to be mediated via the formation of boronate ester linkages between the lectin and glycan subsequent to the initial recognition process and thus preserving its glycan-specificity. Moreover, when attached to magnetic nanoparticles (BAD-lectin@MNPs), 2 to 60-fold improvement on detection sensitivity and enrichment efficiency for specific glycoproteins was observed over the independent use of either lectin or BA. Tested at the level of whole cell lysates for glycoproteomic applications, three different types of BAD-lectin@MNPs exhibited excellent specificities with only 6% overlapping among the 295 N-linked glycopeptides identified. As many as 236 N-linked glycopeptides (80%) were uniquely identified by one of the BAD-lectin@MNPs. These results indicated that the enhanced glycan-selective recognition and binding affinity of BAD-lectin@MNPs will facilitate a complementary identification of the under-explored glycoproteome.

Original languageEnglish
Pages (from-to)8268-8276
Number of pages9
JournalAnalytical Chemistry
Volume85
Issue number17
DOIs
Publication statusPublished - 2013
Externally publishedYes

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Boronic Acids
Lectins
Glycoproteins
Polysaccharides
Glycopeptides
Biocompatible Materials
Labeling
Esters
Carbohydrates

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Lu, Y. W., Chien, C. W., Lin, P. C., Huang, L. D., Chen, C. Y., Wu, S. W., ... Chen, Y. J. (2013). BAD-lectins: Boronic acid-decorated lectins with enhanced binding affinity for the selective enrichment of glycoproteins. Analytical Chemistry, 85(17), 8268-8276. https://doi.org/10.1021/ac401581u

BAD-lectins : Boronic acid-decorated lectins with enhanced binding affinity for the selective enrichment of glycoproteins. / Lu, Ying Wei; Chien, Chih Wei; Lin, Po Chiao; Huang, Li De; Chen, Chang Yang; Wu, Sz Wei; Han, Chia Li; Khoo, Kay Hooi; Lin, Chun Cheng; Chen, Yu Ju.

In: Analytical Chemistry, Vol. 85, No. 17, 2013, p. 8268-8276.

Research output: Contribution to journalArticle

Lu, YW, Chien, CW, Lin, PC, Huang, LD, Chen, CY, Wu, SW, Han, CL, Khoo, KH, Lin, CC & Chen, YJ 2013, 'BAD-lectins: Boronic acid-decorated lectins with enhanced binding affinity for the selective enrichment of glycoproteins', Analytical Chemistry, vol. 85, no. 17, pp. 8268-8276. https://doi.org/10.1021/ac401581u
Lu, Ying Wei ; Chien, Chih Wei ; Lin, Po Chiao ; Huang, Li De ; Chen, Chang Yang ; Wu, Sz Wei ; Han, Chia Li ; Khoo, Kay Hooi ; Lin, Chun Cheng ; Chen, Yu Ju. / BAD-lectins : Boronic acid-decorated lectins with enhanced binding affinity for the selective enrichment of glycoproteins. In: Analytical Chemistry. 2013 ; Vol. 85, No. 17. pp. 8268-8276.
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