Bactericidal activity of soymilk fermentation broth by in vitro and animal models

Yi Ping Chin, Ko Chung Tsui, Mei Chieh Chen, Cheng Yi Wang, Chin Yuh Yang, Yuh Ling Lin

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Soybean fermentation broth (SFB) exhibits potent antibacterial activity against different species of bacteria in in vitro assays and animal models. Four isoflavone compounds-daidzin, genistin, genistein, and daidzein-of SFB were analyzed and quantified by high-performance liquid chromatography. In the in vitro test, daidzin and daidzein had more potent antibacterial activity than genistin. The minimum inhibition concentration values for these bacteria of SFB ranged from 1.25% to 5%, and the minimum bactericidal concentration values of strains ranged from 2.5% to 10%, depending on the species or strain. Vancomycin-resistant Entercoccus faecalis (VRE) strains were also tested for susceptibility to SFB in two species of animal model: the Sprague-Dawley rat and the BALB/c mouse. SFB-fed Sprague-Dawley rats showed excellent elimination efficiency against VRE, close to 99% compared with the phosphate-buffered saline-fed control group. In the BALB/c mouse model, SFB antibacterial activity was 65-80% against VRE compared with the control. In conclusion, SFB contains natural antibacterial substances such as daidzin, genistin, and daidzein that inhibit bacterial growth.

Original languageEnglish
Pages (from-to)520-526
Number of pages7
JournalJournal of Medicinal Food
Issue number6
Publication statusPublished - Jun 1 2012


  • animal models
  • antibacterial activity
  • isoflavones
  • soybean fermentation broth
  • vancomycin-resistant Entercoccus faecalis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics


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