Bacterial mutagenicity, metabolism, and DNA adduct formation by binary mixtures of benzo[a]pyrene and 1-Nitropyrene

H. Lee, S. H. Cherng, T. Y. Liu

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Air pollutants are a complex mixture containing polycyclic organic compounds. Among these are 1-NP and B[a]P, which are important contributors to the mutogenicity of diesel exhaust and airborne particulate matters. To investigate the interaction of a complex mixture of airborne mutagens, the mutagenicity of 1-NP was examined with S. typhimurium TA98 and TA98NR in the presence and absence of B[a]P. B[a]P exhibited a more antagonistic effect on the mutagenicity of 1-NP in strain TA98 than in strain TA98NR. Also studied were (l) the inhibitory effects of B[a]P on the nitroreductive metabolism of 1-NP and (2) DNA adduct formation by 1-NP. Nitroreductase was associated with the metabolism of 1-NP, and was reduced in a dose-dependent manner in a binary mixture of 1-NP and B[a]P. HPLC analysis showed that the amounts of 1-AP and NAAP, the metabolites of 1-NP, were significantly decreased by the addition of B[a]P in mixtures. The results indicate that the antagonistic effect of B[a]P on the mutagenicity of 1-NP is mediated through altering its nitroreductive metabolism.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalEnvironmental and Molecular Mutagenesis
Volume24
Issue number3
DOIs
Publication statusPublished - 1994
Externally publishedYes

Keywords

  • 1-Nitropyrene
  • Benzo[a]pyrene
  • DNA adduct
  • Nitroreductase
  • Salmonella

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Genetics
  • Genetics(clinical)
  • Toxicology
  • Health, Toxicology and Mutagenesis

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