Autophagy induction by a natural ingredient of areca nut

Mei Huei Lin, Shyun Yeu Liu, Young Chau Liu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We recently identified an autophagy-inducing areca nut ingredient (AIAI) in the partially purified 30-100 kDa fraction of areca nut extract (ANE), designated as ANE 30-100K. Before disintegration, most ANE 30-100K-treated cells exhibit rounding morphology, cytoplasmic clearance, and nuclear shrinkage, distinct from arecoline- and cisplatin-induced cellular apoptosis. This unique death pattern is verified to be autophagy by LC3-I cleavage, acidic vesicles, and autophagic vacuoles. As analyzed by Molish's Test, Selinowaff's Test, and thin-layer chromatography, most of the ANE 30-100K constituents are carbohydrates, whereas the protein content of this fraction is less than 1% as assessed by protein assay reagent. The cytotoxicity of ANE 30-100K is further shown to be sensitive to cellulase and proteinase K digestion suggesting AIAI in ANE 30-100K to be a proteoglycan (or glycoprotein). Thus, although ANE contains apoptosis-inducing ingredients such as arecoline, it predominantly triggers autophagic cell death by this natural AIAI.

Original languageEnglish
Pages (from-to)967-968
Number of pages2
JournalAutophagy
Volume4
Issue number7
Publication statusPublished - Oct 1 2008

Fingerprint

Areca
Nuts
Autophagy
Arecoline
Apoptosis
Endopeptidase K
Cellulase
Proteoglycans
Thin Layer Chromatography
Vacuoles
Cisplatin
Digestion
Glycoproteins
Proteins
Carbohydrates

Keywords

  • Apoptosis
  • Areca
  • Autophagy
  • Betel
  • Death

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Lin, M. H., Liu, S. Y., & Liu, Y. C. (2008). Autophagy induction by a natural ingredient of areca nut. Autophagy, 4(7), 967-968.

Autophagy induction by a natural ingredient of areca nut. / Lin, Mei Huei; Liu, Shyun Yeu; Liu, Young Chau.

In: Autophagy, Vol. 4, No. 7, 01.10.2008, p. 967-968.

Research output: Contribution to journalArticle

Lin, MH, Liu, SY & Liu, YC 2008, 'Autophagy induction by a natural ingredient of areca nut', Autophagy, vol. 4, no. 7, pp. 967-968.
Lin MH, Liu SY, Liu YC. Autophagy induction by a natural ingredient of areca nut. Autophagy. 2008 Oct 1;4(7):967-968.
Lin, Mei Huei ; Liu, Shyun Yeu ; Liu, Young Chau. / Autophagy induction by a natural ingredient of areca nut. In: Autophagy. 2008 ; Vol. 4, No. 7. pp. 967-968.
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