Automatic spot detection of CDNA microarray images using mathematical morphology methods

Chiao Ling Shih, Hung Wen Chiu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

cDNA microarray is widely used to identify and quantify different gene-expression for large-scale analysis. In order to extract microarray data precisely from microarray images, robust image processing is indispensable. Most microarray image processing methods are still semiautomatic because of the variations between different arrayers and spots properties. This study attempts to propose an automatic spot detection algorithm using mathematical morphology methods. Watershed transform is the main technique for our spot automatic detection method. To avoid the over-segment problem brought on the sensitive to noises of gradient image used m watershed algorithm, we preprocessed the image using morphological opening operator. Internal and external marker for allocating watershed immersion positions were used to reduce the number of image segments. The result showed that our morphological methods achieved automatic spot detection on non-supervised environment.

Original languageEnglish
Title of host publicationAPBME 2003 - IEEE EMBS Asian-Pacific Conference on Biomedical Engineering 2003
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages70-71
Number of pages2
ISBN (Print)0780379438, 9780780379435
DOIs
Publication statusPublished - 2003
EventIEEE EMBS Asian-Pacific Conference on Biomedical Engineering 2003, APBME 2003 - Kyoto-Osaka-Nara, Japan
Duration: Oct 20 2003Oct 22 2003

Other

OtherIEEE EMBS Asian-Pacific Conference on Biomedical Engineering 2003, APBME 2003
CountryJapan
CityKyoto-Osaka-Nara
Period10/20/0310/22/03

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Keywords

  • CDNA microarray
  • Mathematical morphology
  • Spot detection
  • Watershed transform

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Shih, C. L., & Chiu, H. W. (2003). Automatic spot detection of CDNA microarray images using mathematical morphology methods. In APBME 2003 - IEEE EMBS Asian-Pacific Conference on Biomedical Engineering 2003 (pp. 70-71). [1302588] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/APBME.2003.1302588