Autologous heterotopic transplantation of intact rabbit ovary after frozen banking at -196°C

Chi Huang Chen, Shyi Gen Chen, Gwo Jang Wu, Jaang Wang, Cheng Ping Yu, Jah Yao Liu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objective: To determine the efficiency of intact ovary after cryopreservation and transplantation in a rabbit model for limited longevity by revascularization ischemia after implantation of frozen-thawed ovarian tissue. Transplantation of an intact frozen ovary with microvascular anastomos has been shown to be feasible with modern cryobiology and microsurgery. Design: Animal study. Setting: University-based teaching hospital. Animal(s): Twelve 5-month-old mature female New Zealand White rabbits. Intervention(s): After bilateral oophorectomy with one ovary saved as a control in formalin-fixed and hematoxylin and eosin-stained paraffin sections, the contralateral intact ovary was used for autologous heterotopic transplantation after freezing and thawing. Main Outcome Measure(s): Density of primordial follicles 6 months after transplantation was measured. Hormone levels and vaginal cytology were followed throughout the oophorectomy and transplantation period. Result(s): Ten of 12 rabbits had restored ovarian function 1 week after transplantation of their intact cryopreserved ovary. At the 6-month posttransplantation follow-up, mean primordial follicle density was statistically significantly lower in the experimental than in the control ovaries (13.99 ± 3.21 vs. 18.68 ± 3.86 per high-power field). The remaining two rabbits, which had cracked mesentery fat adjacent to the ovary after thawing, never regained ovarian function. Conclusion(s): With microsurgery, cryopreservation of an intact rabbit ovary followed by autologous transplantation may overcome revascularization-associated ischemia to save primordial follicles and attain reasonable graft longevity.

Original languageEnglish
Pages (from-to)1059-1066
Number of pages8
JournalFertility and Sterility
Volume86
Issue number4 SUPPL.
DOIs
Publication statusPublished - Oct 2006
Externally publishedYes

Fingerprint

Heterotopic Transplantation
Autologous Transplantation
Ovary
Rabbits
Transplantation
Microsurgery
Cryopreservation
Ovariectomy
Ischemia
Mesentery
Hematoxylin
Eosine Yellowish-(YS)
Teaching Hospitals
Paraffin
Formaldehyde
Freezing
Cell Biology
Fats
Outcome Assessment (Health Care)
Hormones

Keywords

  • Cryopreservation
  • microvascular
  • ovary
  • transplantation

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Autologous heterotopic transplantation of intact rabbit ovary after frozen banking at -196°C. / Chen, Chi Huang; Chen, Shyi Gen; Wu, Gwo Jang; Wang, Jaang; Yu, Cheng Ping; Liu, Jah Yao.

In: Fertility and Sterility, Vol. 86, No. 4 SUPPL., 10.2006, p. 1059-1066.

Research output: Contribution to journalArticle

Chen, Chi Huang ; Chen, Shyi Gen ; Wu, Gwo Jang ; Wang, Jaang ; Yu, Cheng Ping ; Liu, Jah Yao. / Autologous heterotopic transplantation of intact rabbit ovary after frozen banking at -196°C. In: Fertility and Sterility. 2006 ; Vol. 86, No. 4 SUPPL. pp. 1059-1066.
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