Autocrine IL-6 regulates GRO-α production in thymic epithelial cells

Yau Lin Tseng, Ming Ho Wu, Hsin Chiao Yang, Chi Yun Wang, Chiou Feng Lin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Thymic interleukin-6 (IL-6), which is produced primarily by thymic epithelial cells, is an important cytokine for T cell growth and differentiation in the thymus. In the present study, we investigated the effects of autocrine IL-6 on the cytokines in primary cultures of human thymic epithelial cells. Using an antibody array and ELISA, in addition to IL-8 and MCP-1, growth-related oncogene-α (GRO-α) was subsequently expressed after IL-6 production. Either IL-6 or GRO-α was time-dependently and strongly expressed in medium with and without serum. Neutralizing IL-6 significantly reduced GRO-α production. Inhibiting Janus kinase 2 (Jak2) spontaneously blocked GRO-α production with or without IL-6 stimulation. We conclude that primary human thymic epithelial cells produce GRO-α and that its expression is regulated primarily by autocrine IL-6 and IL-6-activated Jak2 signaling.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalCytokine
Volume51
Issue number2
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

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Keywords

  • GRO-α
  • IL-6
  • Jak2
  • Thymic epithelial cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology
  • Medicine(all)

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