Augmentation of murine lymphokine (rIL-2)-activated killer cell activity by indomethacin.

T. Y. Chao, H. Ohnishi, T. M. Chu

Research output: Contribution to journalArticle

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Abstract

The effect of indomethacin on murine lymphokine-activated killer (LAK) cell activity was investigated using a natural killer-resistant, spontaneously developed, weakly immunogenic, and highly tumorigenic syngeneic murine mammary adenocarcinoma, mimicking that of human disease, as the target. When used in combination with human recombinant interleukin-2 (rIL-2), indomethacin was found to augment LAK cell activity, which was generated from culture of the normal mouse splenocytes with rIL-2, as compared to that with rIL-2 alone. This increase in LAK cell activity was shown to be indomethacin dose-dependent, and was demonstrated only when indomethacin was added to the rIL-2-containing medium at the beginning of culture. The enhancement of LAK cell activity by indomethacin was abrogated when the nylon-wool nonadherent "macrophage-poor" splenocytes were incubated with rIL-2 plus indomethacin. These results indicated that the rIL-2-induced LAK cell activity generated from murine splenocytes could be augmented by indomethacin, and the macrophages may be involved as the mediator.

Original languageEnglish
Pages (from-to)318-322
Number of pages5
JournalMolecular biotherapy
Volume1
Issue number6
Publication statusPublished - 1989
Externally publishedYes

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Lymphokines
Indomethacin
Lymphokine-Activated Killer Cells
Interleukin-2
Macrophages
Wool
Nylons
Adenocarcinoma
Breast

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Augmentation of murine lymphokine (rIL-2)-activated killer cell activity by indomethacin. / Chao, T. Y.; Ohnishi, H.; Chu, T. M.

In: Molecular biotherapy, Vol. 1, No. 6, 1989, p. 318-322.

Research output: Contribution to journalArticle

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