Attenuation of the extract from Moringa oleifera on monocrotaline-induced pulmonary hypertension in rats.

Kang Hu Chen, Yi Jui Chen, Chao Hsun Yang, Kuo Wei Liu, Junn Liang Chang, Shwu Fen Pan, Tzer Bin Lin, Mei Jung Chen

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to determine the effects of an extract from Moringa oleifera (MO) on the development of monocrotaline (MCT)-induced pulmonary hypertension (PH) in Wistar rats. An ethanol extraction was performed on dried MO leaves, and HPLC analysis identified niaziridin and niazirin in the extract. PH was induced with a single subcutaneous injection of MCT (60 mg/kg) which resulted in increases in pulmonary arterial blood pressure (Ppa) and in thickening of the pulmonary arterial medial layer in the rats. Three weeks after induction, acute administration of the MO extract to the rats decreased Ppa in a dose-dependent manner that reached statistical significance at a dose of 4.5 mg of freeze-dried extract per kg body weight. The reduction in Ppa suggested that the extract directly relaxed the pulmonary arteries. To assay the effects of chronic administration of the MO extract on PH, control, MCT and MCT+MO groups were designated. Rats in the control group received a saline injection; the MCT and MCT+MO groups received MCT to induce PH. During the third week after MCT treatment, the MCT+MO group received daily i.p. injections of the MO extract (4.5 mg of freeze-dried extract/kg of body weight). Compared to the control group, the MCT group had higher Ppa and thicker medial layers in the pulmonary arteries. Chronic treatments with the MO extract reversed the MCT-induced changes. Additionally, the MCT group had a significant elevation in superoxide dismutase activity when normalized by the MO extract treatments. In conclusion, the MO extract successfully attenuated the development of PH via direct vasodilatation and a potential increase in antioxidant activity.

Original languageEnglish
Pages (from-to)22-30
Number of pages9
JournalChinese Journal of Physiology
Volume55
Issue number1
Publication statusPublished - Feb 29 2012
Externally publishedYes

Fingerprint

Moringa oleifera
Monocrotaline
Pulmonary Hypertension
Pulmonary Artery
Body Weight
Lung
Control Groups
Injections
Subcutaneous Injections
Vasodilation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Chen, K. H., Chen, Y. J., Yang, C. H., Liu, K. W., Chang, J. L., Pan, S. F., ... Chen, M. J. (2012). Attenuation of the extract from Moringa oleifera on monocrotaline-induced pulmonary hypertension in rats. Chinese Journal of Physiology, 55(1), 22-30.

Attenuation of the extract from Moringa oleifera on monocrotaline-induced pulmonary hypertension in rats. / Chen, Kang Hu; Chen, Yi Jui; Yang, Chao Hsun; Liu, Kuo Wei; Chang, Junn Liang; Pan, Shwu Fen; Lin, Tzer Bin; Chen, Mei Jung.

In: Chinese Journal of Physiology, Vol. 55, No. 1, 29.02.2012, p. 22-30.

Research output: Contribution to journalArticle

Chen, KH, Chen, YJ, Yang, CH, Liu, KW, Chang, JL, Pan, SF, Lin, TB & Chen, MJ 2012, 'Attenuation of the extract from Moringa oleifera on monocrotaline-induced pulmonary hypertension in rats.', Chinese Journal of Physiology, vol. 55, no. 1, pp. 22-30.
Chen, Kang Hu ; Chen, Yi Jui ; Yang, Chao Hsun ; Liu, Kuo Wei ; Chang, Junn Liang ; Pan, Shwu Fen ; Lin, Tzer Bin ; Chen, Mei Jung. / Attenuation of the extract from Moringa oleifera on monocrotaline-induced pulmonary hypertension in rats. In: Chinese Journal of Physiology. 2012 ; Vol. 55, No. 1. pp. 22-30.
@article{905eb69222b74bb28b14ab90b98e5659,
title = "Attenuation of the extract from Moringa oleifera on monocrotaline-induced pulmonary hypertension in rats.",
abstract = "The purpose of this study was to determine the effects of an extract from Moringa oleifera (MO) on the development of monocrotaline (MCT)-induced pulmonary hypertension (PH) in Wistar rats. An ethanol extraction was performed on dried MO leaves, and HPLC analysis identified niaziridin and niazirin in the extract. PH was induced with a single subcutaneous injection of MCT (60 mg/kg) which resulted in increases in pulmonary arterial blood pressure (Ppa) and in thickening of the pulmonary arterial medial layer in the rats. Three weeks after induction, acute administration of the MO extract to the rats decreased Ppa in a dose-dependent manner that reached statistical significance at a dose of 4.5 mg of freeze-dried extract per kg body weight. The reduction in Ppa suggested that the extract directly relaxed the pulmonary arteries. To assay the effects of chronic administration of the MO extract on PH, control, MCT and MCT+MO groups were designated. Rats in the control group received a saline injection; the MCT and MCT+MO groups received MCT to induce PH. During the third week after MCT treatment, the MCT+MO group received daily i.p. injections of the MO extract (4.5 mg of freeze-dried extract/kg of body weight). Compared to the control group, the MCT group had higher Ppa and thicker medial layers in the pulmonary arteries. Chronic treatments with the MO extract reversed the MCT-induced changes. Additionally, the MCT group had a significant elevation in superoxide dismutase activity when normalized by the MO extract treatments. In conclusion, the MO extract successfully attenuated the development of PH via direct vasodilatation and a potential increase in antioxidant activity.",
author = "Chen, {Kang Hu} and Chen, {Yi Jui} and Yang, {Chao Hsun} and Liu, {Kuo Wei} and Chang, {Junn Liang} and Pan, {Shwu Fen} and Lin, {Tzer Bin} and Chen, {Mei Jung}",
year = "2012",
month = "2",
day = "29",
language = "English",
volume = "55",
pages = "22--30",
journal = "Chinese Journal of Physiology",
issn = "0304-4920",
publisher = "Chinese Physiological Society",
number = "1",

}

TY - JOUR

T1 - Attenuation of the extract from Moringa oleifera on monocrotaline-induced pulmonary hypertension in rats.

AU - Chen, Kang Hu

AU - Chen, Yi Jui

AU - Yang, Chao Hsun

AU - Liu, Kuo Wei

AU - Chang, Junn Liang

AU - Pan, Shwu Fen

AU - Lin, Tzer Bin

AU - Chen, Mei Jung

PY - 2012/2/29

Y1 - 2012/2/29

N2 - The purpose of this study was to determine the effects of an extract from Moringa oleifera (MO) on the development of monocrotaline (MCT)-induced pulmonary hypertension (PH) in Wistar rats. An ethanol extraction was performed on dried MO leaves, and HPLC analysis identified niaziridin and niazirin in the extract. PH was induced with a single subcutaneous injection of MCT (60 mg/kg) which resulted in increases in pulmonary arterial blood pressure (Ppa) and in thickening of the pulmonary arterial medial layer in the rats. Three weeks after induction, acute administration of the MO extract to the rats decreased Ppa in a dose-dependent manner that reached statistical significance at a dose of 4.5 mg of freeze-dried extract per kg body weight. The reduction in Ppa suggested that the extract directly relaxed the pulmonary arteries. To assay the effects of chronic administration of the MO extract on PH, control, MCT and MCT+MO groups were designated. Rats in the control group received a saline injection; the MCT and MCT+MO groups received MCT to induce PH. During the third week after MCT treatment, the MCT+MO group received daily i.p. injections of the MO extract (4.5 mg of freeze-dried extract/kg of body weight). Compared to the control group, the MCT group had higher Ppa and thicker medial layers in the pulmonary arteries. Chronic treatments with the MO extract reversed the MCT-induced changes. Additionally, the MCT group had a significant elevation in superoxide dismutase activity when normalized by the MO extract treatments. In conclusion, the MO extract successfully attenuated the development of PH via direct vasodilatation and a potential increase in antioxidant activity.

AB - The purpose of this study was to determine the effects of an extract from Moringa oleifera (MO) on the development of monocrotaline (MCT)-induced pulmonary hypertension (PH) in Wistar rats. An ethanol extraction was performed on dried MO leaves, and HPLC analysis identified niaziridin and niazirin in the extract. PH was induced with a single subcutaneous injection of MCT (60 mg/kg) which resulted in increases in pulmonary arterial blood pressure (Ppa) and in thickening of the pulmonary arterial medial layer in the rats. Three weeks after induction, acute administration of the MO extract to the rats decreased Ppa in a dose-dependent manner that reached statistical significance at a dose of 4.5 mg of freeze-dried extract per kg body weight. The reduction in Ppa suggested that the extract directly relaxed the pulmonary arteries. To assay the effects of chronic administration of the MO extract on PH, control, MCT and MCT+MO groups were designated. Rats in the control group received a saline injection; the MCT and MCT+MO groups received MCT to induce PH. During the third week after MCT treatment, the MCT+MO group received daily i.p. injections of the MO extract (4.5 mg of freeze-dried extract/kg of body weight). Compared to the control group, the MCT group had higher Ppa and thicker medial layers in the pulmonary arteries. Chronic treatments with the MO extract reversed the MCT-induced changes. Additionally, the MCT group had a significant elevation in superoxide dismutase activity when normalized by the MO extract treatments. In conclusion, the MO extract successfully attenuated the development of PH via direct vasodilatation and a potential increase in antioxidant activity.

UR - http://www.scopus.com/inward/record.url?scp=84859922797&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859922797&partnerID=8YFLogxK

M3 - Article

C2 - 22242951

VL - 55

SP - 22

EP - 30

JO - Chinese Journal of Physiology

JF - Chinese Journal of Physiology

SN - 0304-4920

IS - 1

ER -