Attenuated cardiac mitochondrial-dependent apoptotic effects by Li-Fu formula in hamsters fed with a hypercholesterol diet

Bor Show Tzang, Wei Wen Kuo, Tsai Ching Hsu, Mei Haung Chain, Chao Hung Lai, Wen Hong Wang, Fuu Jen Tsai, Chang Hai Tsai, Chieh His Wu, Chih Yang Huang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Apoptosis involves in the pathogenesis of various cardiac abnormalities. This study intends to evaluate the effects of Li-Fu formula on cardiac apoptosis induced by hyper-cholesterol diet. Twenty-four male Golden Syrian hamsters were randomly divided into Control, Cholesterol and Li-Fu formula groups. Histopathological analysis, western blotting and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed to measure the effects of Li-Fu formula on left ventricle. Significantly reduced TUNEL-positive cells and mitochondria- dependent apoptosis were observed in the left ventricle of hamsters from Li-Fu formula group compared to the Cholesterol group. Additionally, induced cardiac insulin like growth factor I receptor (IGFIR)-dependent survival pathway was detected in the Li-Fu formula group compared to the Cholesterol group. Besides, minor fibrosis, increased collagen deposition, and myofibril disarray was detected in the Cholesterol group, whereas the reductions of collagen deposition and myofibril disarray were observed in the Li-Fu formula group. This study demonstrated that Li-Fu formula not only reduced the mitochondria-dependent apoptosis and fibrosis, but also enhanced the IGF-I survival pathway in the left ventricle from high cholesterol-fed hamsters. We suggest the protective effects of Li-Fu formula on cardiac apoptosis and therapeutic potentials against cardiovascular disease.

Original languageEnglish
Article number530345
JournalEvidence-based Complementary and Alternative Medicine
Volume2011
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Cricetinae
Cholesterol
Diet
Apoptosis
Heart Ventricles
Myofibrils
Mitochondria
Fibrosis
Collagen
IGF Type 1 Receptor
DNA Nucleotidylexotransferase
Mesocricetus
Transferases
Insulin-Like Growth Factor I
Cardiovascular Diseases
Western Blotting

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Attenuated cardiac mitochondrial-dependent apoptotic effects by Li-Fu formula in hamsters fed with a hypercholesterol diet. / Tzang, Bor Show; Kuo, Wei Wen; Hsu, Tsai Ching; Chain, Mei Haung; Lai, Chao Hung; Wang, Wen Hong; Tsai, Fuu Jen; Tsai, Chang Hai; Wu, Chieh His; Huang, Chih Yang.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2011, 530345, 2011.

Research output: Contribution to journalArticle

Tzang, Bor Show ; Kuo, Wei Wen ; Hsu, Tsai Ching ; Chain, Mei Haung ; Lai, Chao Hung ; Wang, Wen Hong ; Tsai, Fuu Jen ; Tsai, Chang Hai ; Wu, Chieh His ; Huang, Chih Yang. / Attenuated cardiac mitochondrial-dependent apoptotic effects by Li-Fu formula in hamsters fed with a hypercholesterol diet. In: Evidence-based Complementary and Alternative Medicine. 2011 ; Vol. 2011.
@article{cb5bfcf67bd949cf9c48afd2ebfd315b,
title = "Attenuated cardiac mitochondrial-dependent apoptotic effects by Li-Fu formula in hamsters fed with a hypercholesterol diet",
abstract = "Apoptosis involves in the pathogenesis of various cardiac abnormalities. This study intends to evaluate the effects of Li-Fu formula on cardiac apoptosis induced by hyper-cholesterol diet. Twenty-four male Golden Syrian hamsters were randomly divided into Control, Cholesterol and Li-Fu formula groups. Histopathological analysis, western blotting and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed to measure the effects of Li-Fu formula on left ventricle. Significantly reduced TUNEL-positive cells and mitochondria- dependent apoptosis were observed in the left ventricle of hamsters from Li-Fu formula group compared to the Cholesterol group. Additionally, induced cardiac insulin like growth factor I receptor (IGFIR)-dependent survival pathway was detected in the Li-Fu formula group compared to the Cholesterol group. Besides, minor fibrosis, increased collagen deposition, and myofibril disarray was detected in the Cholesterol group, whereas the reductions of collagen deposition and myofibril disarray were observed in the Li-Fu formula group. This study demonstrated that Li-Fu formula not only reduced the mitochondria-dependent apoptosis and fibrosis, but also enhanced the IGF-I survival pathway in the left ventricle from high cholesterol-fed hamsters. We suggest the protective effects of Li-Fu formula on cardiac apoptosis and therapeutic potentials against cardiovascular disease.",
author = "Tzang, {Bor Show} and Kuo, {Wei Wen} and Hsu, {Tsai Ching} and Chain, {Mei Haung} and Lai, {Chao Hung} and Wang, {Wen Hong} and Tsai, {Fuu Jen} and Tsai, {Chang Hai} and Wu, {Chieh His} and Huang, {Chih Yang}",
year = "2011",
doi = "10.1093/ecam/nep182",
language = "English",
volume = "2011",
journal = "Evidence-based Complementary and Alternative Medicine",
issn = "1741-427X",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Attenuated cardiac mitochondrial-dependent apoptotic effects by Li-Fu formula in hamsters fed with a hypercholesterol diet

AU - Tzang, Bor Show

AU - Kuo, Wei Wen

AU - Hsu, Tsai Ching

AU - Chain, Mei Haung

AU - Lai, Chao Hung

AU - Wang, Wen Hong

AU - Tsai, Fuu Jen

AU - Tsai, Chang Hai

AU - Wu, Chieh His

AU - Huang, Chih Yang

PY - 2011

Y1 - 2011

N2 - Apoptosis involves in the pathogenesis of various cardiac abnormalities. This study intends to evaluate the effects of Li-Fu formula on cardiac apoptosis induced by hyper-cholesterol diet. Twenty-four male Golden Syrian hamsters were randomly divided into Control, Cholesterol and Li-Fu formula groups. Histopathological analysis, western blotting and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed to measure the effects of Li-Fu formula on left ventricle. Significantly reduced TUNEL-positive cells and mitochondria- dependent apoptosis were observed in the left ventricle of hamsters from Li-Fu formula group compared to the Cholesterol group. Additionally, induced cardiac insulin like growth factor I receptor (IGFIR)-dependent survival pathway was detected in the Li-Fu formula group compared to the Cholesterol group. Besides, minor fibrosis, increased collagen deposition, and myofibril disarray was detected in the Cholesterol group, whereas the reductions of collagen deposition and myofibril disarray were observed in the Li-Fu formula group. This study demonstrated that Li-Fu formula not only reduced the mitochondria-dependent apoptosis and fibrosis, but also enhanced the IGF-I survival pathway in the left ventricle from high cholesterol-fed hamsters. We suggest the protective effects of Li-Fu formula on cardiac apoptosis and therapeutic potentials against cardiovascular disease.

AB - Apoptosis involves in the pathogenesis of various cardiac abnormalities. This study intends to evaluate the effects of Li-Fu formula on cardiac apoptosis induced by hyper-cholesterol diet. Twenty-four male Golden Syrian hamsters were randomly divided into Control, Cholesterol and Li-Fu formula groups. Histopathological analysis, western blotting and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed to measure the effects of Li-Fu formula on left ventricle. Significantly reduced TUNEL-positive cells and mitochondria- dependent apoptosis were observed in the left ventricle of hamsters from Li-Fu formula group compared to the Cholesterol group. Additionally, induced cardiac insulin like growth factor I receptor (IGFIR)-dependent survival pathway was detected in the Li-Fu formula group compared to the Cholesterol group. Besides, minor fibrosis, increased collagen deposition, and myofibril disarray was detected in the Cholesterol group, whereas the reductions of collagen deposition and myofibril disarray were observed in the Li-Fu formula group. This study demonstrated that Li-Fu formula not only reduced the mitochondria-dependent apoptosis and fibrosis, but also enhanced the IGF-I survival pathway in the left ventricle from high cholesterol-fed hamsters. We suggest the protective effects of Li-Fu formula on cardiac apoptosis and therapeutic potentials against cardiovascular disease.

UR - http://www.scopus.com/inward/record.url?scp=79953275697&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953275697&partnerID=8YFLogxK

U2 - 10.1093/ecam/nep182

DO - 10.1093/ecam/nep182

M3 - Article

C2 - 19939950

AN - SCOPUS:79953275697

VL - 2011

JO - Evidence-based Complementary and Alternative Medicine

JF - Evidence-based Complementary and Alternative Medicine

SN - 1741-427X

M1 - 530345

ER -