Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V

Kohei Murata, Eiji Miyoshi, Shinji Ihara, Shingo Noura, Masao Kameyama, Osamu Ishikawa, Yuichiro Doki, Terumasa Yamada, Hiroaki Ohigashi, Yo Sasaki, Masahiko Higashiyama, Takehiko Tarui, Yoshikazu Takada, Reiji Kannagi, Naoyuki Taniguchi, Shingi Imaoka

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Expression of N-acetylglucosaminyltransferase V (GnT-V) in colon cancer has been shown to be related to hematogenous metastasis and poor prognosis. To investigate the mechanism by which cancer cells expressing GnT-V metastasize to distant organs, we established GnT-Voverexpressing DLD-1 and WiDr cells (human colon cancer cell lines) by transfecting them with a GnT-V expression vector. Attachment to endothelial cells expressing E-selectin was studied, and expression of the E-selectin ligand, sialyl Lewis x, in colon cancer cells was investigated. Both of the cell lines showed reduced adhesion to fibronectin as compared with mock transfectants. In contrast, attachment to human umbilical vein endothelial cells expressing E-selectin was significantly enhanced by GnT-V expression (p

Original languageEnglish
Pages (from-to)492-501
Number of pages10
JournalOncology
Volume66
Issue number6
DOIs
Publication statusPublished - 2004
Externally publishedYes

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Keywords

  • Colon cancer
  • E-Selectin
  • Mannoside acetylglucosaminyl transferase 5
  • N-acetylglucosaminyltransferase V
  • Sialyl Lewis x

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Murata, K., Miyoshi, E., Ihara, S., Noura, S., Kameyama, M., Ishikawa, O., ... Imaoka, S. (2004). Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V. Oncology, 66(6), 492-501. https://doi.org/10.1159/000079504