Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V

Kohei Murata; Eiji Miyoshi; Shinji Ihara; Shingo Noura; Masao Kameyama; Osamu Ishikawa; Yuichiro Doki; Terumasa Yamada; Hiroaki Ohigashi; Yo Sasaki; Masahiko Higashiyama; Takehiko Tarui; Yoshikazu Takada; Reiji Kannagi; Naoyuki Taniguchi; Shingi Imaoka

doi:10.1159/000079504

Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V

Kohei Murata, Eiji Miyoshi, Shinji Ihara, Shingo Noura, Masao Kameyama, Osamu Ishikawa, Yuichiro Doki, Terumasa Yamada, Hiroaki Ohigashi, Yo Sasaki, Masahiko Higashiyama, Takehiko Tarui, Yoshikazu Takada, Reiji Kannagi, Naoyuki Taniguchi, Shingi Imaoka

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Expression of N-acetylglucosaminyltransferase V (GnT-V) in colon cancer has been shown to be related to hematogenous metastasis and poor prognosis. To investigate the mechanism by which cancer cells expressing GnT-V metastasize to distant organs, we established GnT-Voverexpressing DLD-1 and WiDr cells (human colon cancer cell lines) by transfecting them with a GnT-V expression vector. Attachment to endothelial cells expressing E-selectin was studied, and expression of the E-selectin ligand, sialyl Lewis x, in colon cancer cells was investigated. Both of the cell lines showed reduced adhesion to fibronectin as compared with mock transfectants. In contrast, attachment to human umbilical vein endothelial cells expressing E-selectin was significantly enhanced by GnT-V expression (p

Original language	English
Pages (from-to)	492-501
Number of pages	10
Journal	Oncology
Volume	66
Issue number	6
DOIs	https://doi.org/10.1159/000079504
Publication status	Published - 2004
Externally published	Yes

Keywords

Colon cancer
E-Selectin
Mannoside acetylglucosaminyl transferase 5
N-acetylglucosaminyltransferase V
Sialyl Lewis x

ASJC Scopus subject areas

Cancer Research
Oncology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1159/000079504

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Murata, K., Miyoshi, E., Ihara, S., Noura, S., Kameyama, M., Ishikawa, O., Doki, Y., Yamada, T., Ohigashi, H., Sasaki, Y., Higashiyama, M., Tarui, T., Takada, Y., Kannagi, R., Taniguchi, N., & Imaoka, S. (2004). Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V. Oncology, 66(6), 492-501. https://doi.org/10.1159/000079504

Murata, K, Miyoshi, E, Ihara, S, Noura, S, Kameyama, M, Ishikawa, O, Doki, Y, Yamada, T, Ohigashi, H, Sasaki, Y, Higashiyama, M, Tarui, T, Takada, Y, Kannagi, R, Taniguchi, N & Imaoka, S 2004, 'Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V', Oncology, vol. 66, no. 6, pp. 492-501. https://doi.org/10.1159/000079504

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title = "Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V",

abstract = "Expression of N-acetylglucosaminyltransferase V (GnT-V) in colon cancer has been shown to be related to hematogenous metastasis and poor prognosis. To investigate the mechanism by which cancer cells expressing GnT-V metastasize to distant organs, we established GnT-Voverexpressing DLD-1 and WiDr cells (human colon cancer cell lines) by transfecting them with a GnT-V expression vector. Attachment to endothelial cells expressing E-selectin was studied, and expression of the E-selectin ligand, sialyl Lewis x, in colon cancer cells was investigated. Both of the cell lines showed reduced adhesion to fibronectin as compared with mock transfectants. In contrast, attachment to human umbilical vein endothelial cells expressing E-selectin was significantly enhanced by GnT-V expression (p ",

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author = "Kohei Murata and Eiji Miyoshi and Shinji Ihara and Shingo Noura and Masao Kameyama and Osamu Ishikawa and Yuichiro Doki and Terumasa Yamada and Hiroaki Ohigashi and Yo Sasaki and Masahiko Higashiyama and Takehiko Tarui and Yoshikazu Takada and Reiji Kannagi and Naoyuki Taniguchi and Shingi Imaoka",

year = "2004",

doi = "10.1159/000079504",

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T1 - Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V

AU - Murata, Kohei

AU - Miyoshi, Eiji

AU - Ihara, Shinji

AU - Noura, Shingo

AU - Kameyama, Masao

AU - Ishikawa, Osamu

AU - Doki, Yuichiro

AU - Yamada, Terumasa

AU - Ohigashi, Hiroaki

AU - Sasaki, Yo

AU - Higashiyama, Masahiko

AU - Tarui, Takehiko

AU - Takada, Yoshikazu

AU - Kannagi, Reiji

AU - Taniguchi, Naoyuki

AU - Imaoka, Shingi

PY - 2004

Y1 - 2004

N2 - Expression of N-acetylglucosaminyltransferase V (GnT-V) in colon cancer has been shown to be related to hematogenous metastasis and poor prognosis. To investigate the mechanism by which cancer cells expressing GnT-V metastasize to distant organs, we established GnT-Voverexpressing DLD-1 and WiDr cells (human colon cancer cell lines) by transfecting them with a GnT-V expression vector. Attachment to endothelial cells expressing E-selectin was studied, and expression of the E-selectin ligand, sialyl Lewis x, in colon cancer cells was investigated. Both of the cell lines showed reduced adhesion to fibronectin as compared with mock transfectants. In contrast, attachment to human umbilical vein endothelial cells expressing E-selectin was significantly enhanced by GnT-V expression (p

AB - Expression of N-acetylglucosaminyltransferase V (GnT-V) in colon cancer has been shown to be related to hematogenous metastasis and poor prognosis. To investigate the mechanism by which cancer cells expressing GnT-V metastasize to distant organs, we established GnT-Voverexpressing DLD-1 and WiDr cells (human colon cancer cell lines) by transfecting them with a GnT-V expression vector. Attachment to endothelial cells expressing E-selectin was studied, and expression of the E-selectin ligand, sialyl Lewis x, in colon cancer cells was investigated. Both of the cell lines showed reduced adhesion to fibronectin as compared with mock transfectants. In contrast, attachment to human umbilical vein endothelial cells expressing E-selectin was significantly enhanced by GnT-V expression (p

KW - Colon cancer

KW - E-Selectin

KW - Mannoside acetylglucosaminyl transferase 5

KW - N-acetylglucosaminyltransferase V

KW - Sialyl Lewis x

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Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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