Atrial fibrillation is associated with accumulation of aging-related common type mitochondrial DNA deletion mutation in human atrial tissue

Ling Ping Lai, Chien Chen Tsai, Ming Jai Su, Jiunn Lee Lin, Yih Sharng Chen, Yung Zu Tseng, Shoei K.Stephen Huang

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Study objective: Accumulation of somatic mutations of mitochondrial DNA (mtDNA) contributes to the aging process and progressive organ dysfunction. We investigated the mitochondrial DNA with 4977-base-pair mtDNA deletion mutation (mtDNA4977) in human atrial tissue and correlated the amount of mtDNA4977 to clinical atrial fibrillation (AF). Methods and results: Atrial tissue from the right atrial appendage was obtained in 88 patients during open-heart surgery (22 children/adolescents and 66 adults). The amount of mtDNA4977 was measured using a nested polymerase chain reaction protocol and normalized to wild-type mtDNA. We found that the mtDNA4977 was absent in all 22 pediatric/adolescent patients. In the adult group, the relative amount of mtDNA4977 was significantly higher in patients with AF than in patients without AF (0.55 ± 0.26 vs 0.35 ± 0.29, p < 0.007) [mean ± SD]. The amount of mtDNA4977 was also positively associated with age (r = 0.29, p < 0.01). Left and right atrial pressures, left atrial dimension, hypertension, and cardiac diagnosis did not influence the amount of mtDNA4977 significantly. Further multivariate analysis showed that both aging and AF contributed independently to the accumulation of mtDNA4977. Conclusion: AF is associated with an increase of mtDNA4977. This change is similar to the aging process of atrial tissue and might contribute to atrial dysfunction in AF.

Original languageEnglish
Pages (from-to)539-544
Number of pages6
JournalChest
Volume123
Issue number2
DOIs
Publication statusPublished - Feb 1 2003
Externally publishedYes

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Sequence Deletion
Mitochondrial DNA
Atrial Fibrillation
Atrial Appendage
Atrial Pressure
Base Pairing
Thoracic Surgery
Multivariate Analysis
Pediatrics
Hypertension
Polymerase Chain Reaction

Keywords

  • Atrial fibrillation
  • Mitochondria
  • Molecular biology
  • Mutation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Atrial fibrillation is associated with accumulation of aging-related common type mitochondrial DNA deletion mutation in human atrial tissue. / Lai, Ling Ping; Tsai, Chien Chen; Su, Ming Jai; Lin, Jiunn Lee; Chen, Yih Sharng; Tseng, Yung Zu; Huang, Shoei K.Stephen.

In: Chest, Vol. 123, No. 2, 01.02.2003, p. 539-544.

Research output: Contribution to journalArticle

Lai, Ling Ping ; Tsai, Chien Chen ; Su, Ming Jai ; Lin, Jiunn Lee ; Chen, Yih Sharng ; Tseng, Yung Zu ; Huang, Shoei K.Stephen. / Atrial fibrillation is associated with accumulation of aging-related common type mitochondrial DNA deletion mutation in human atrial tissue. In: Chest. 2003 ; Vol. 123, No. 2. pp. 539-544.
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