Atopic dermatitis is associated with a functional mutation in the promoter of the C-C chemokine RANTES

Renate G. Nickel, Vincenzo Casolaro, Ulrich Wahn, Kirsten Beyer, Kathleen C. Barnes, Beverly S. Plunkett, Linda R. Freidhoff, Claudia Sengler, James R. Plitt, Robert P. Schleimer, Luis Caraballo, Raana P. Naidu, Paul N. Levett, Terri H. Beaty, Shau Ku Huang

Research output: Contribution to journalArticle

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Abstract

Up-regulation of C-C chemokine expression characterizes allergic inflammation and atopic diseases. A functional mutation in the proximal promoter of the RANTES gene has been identified, which results in a new consensus binding site for the GATA transcription factor family. A higher frequency of this allele was observed in individuals of African descent compared with Caucasian subjects (p < 0.00001). The mutant allele was associated with atopic dermatitis in children of the German Multicenter Allergy Study (MAS-90; p < 0.037), but not with asthma. Transient transfections of the human mast cell line HMC-1 and the T cell line Jurkat with reporter vectors driven by either the mutant or wild-type RANTES promoter showed an up to 8-fold higher constitutive transcriptional activity of the mutant promoter. This is the first report to our knowledge of a functional mutation in a chemokine gene promoter. Our findings suggest that the mutation contributes to the development of atopic dermatitis. Its potential role in other inflammatory and infectious disorders, particularly among individuals of African ancestry, remains to be determined.

Original languageEnglish
Pages (from-to)1612-1616
Number of pages5
JournalJournal of Immunology
Volume164
Issue number3
DOIs
Publication statusPublished - Feb 1 2000
Externally publishedYes

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Chemokine CCL5
CC Chemokines
Atopic Dermatitis
Mutation
GATA Transcription Factors
Cell Line
Chemokines
Gene Frequency
Mast Cells
Genes
Multicenter Studies
Transfection
Hypersensitivity
Up-Regulation
Asthma
Alleles
Binding Sites
Inflammation
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Atopic dermatitis is associated with a functional mutation in the promoter of the C-C chemokine RANTES. / Nickel, Renate G.; Casolaro, Vincenzo; Wahn, Ulrich; Beyer, Kirsten; Barnes, Kathleen C.; Plunkett, Beverly S.; Freidhoff, Linda R.; Sengler, Claudia; Plitt, James R.; Schleimer, Robert P.; Caraballo, Luis; Naidu, Raana P.; Levett, Paul N.; Beaty, Terri H.; Huang, Shau Ku.

In: Journal of Immunology, Vol. 164, No. 3, 01.02.2000, p. 1612-1616.

Research output: Contribution to journalArticle

Nickel, RG, Casolaro, V, Wahn, U, Beyer, K, Barnes, KC, Plunkett, BS, Freidhoff, LR, Sengler, C, Plitt, JR, Schleimer, RP, Caraballo, L, Naidu, RP, Levett, PN, Beaty, TH & Huang, SK 2000, 'Atopic dermatitis is associated with a functional mutation in the promoter of the C-C chemokine RANTES', Journal of Immunology, vol. 164, no. 3, pp. 1612-1616. https://doi.org/10.4049/jimmunol.164.3.1612
Nickel, Renate G. ; Casolaro, Vincenzo ; Wahn, Ulrich ; Beyer, Kirsten ; Barnes, Kathleen C. ; Plunkett, Beverly S. ; Freidhoff, Linda R. ; Sengler, Claudia ; Plitt, James R. ; Schleimer, Robert P. ; Caraballo, Luis ; Naidu, Raana P. ; Levett, Paul N. ; Beaty, Terri H. ; Huang, Shau Ku. / Atopic dermatitis is associated with a functional mutation in the promoter of the C-C chemokine RANTES. In: Journal of Immunology. 2000 ; Vol. 164, No. 3. pp. 1612-1616.
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