Association of Mycobacterium tuberculosis genotypes and clinical and epidemiological features - A multi-center study in Taiwan

Shiang Fen Huang, Wei Juin Su, Horng Yunn Dou, Jia Yih Feng, Yu Chin Lee, Ruay Ming Huang, Ching Hsiung Lin, Jhi Jhu Hwang, Jen Jyh Lee, Ming Chih Yu

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Abstract

Genotypes of Mycobacterium tuberculosis (MTB) are related to the geographic origin of the patients and population migration. The relationship between genotypes of MTB and clinical presentations has mainly focused on transmission of multi-drug resistant MTB strain in population. This study aimed to investigate the molecular epidemiology and dynamic change of MTB genotypes in Taiwan, and their association with clinical presentation among patients with pulmonary tuberculosis. A multi-center, two-year study which enrolled 516 patients with 516 MTB isolates was conducted, including: (1) 254 isolates from northern Taiwan; (2) 38 isolates from mid-western Taiwan; (3) 211 isolates from southern Taiwan; and (4) 13 isolates from the east coast of Taiwan. The isolates were genotyped with spoligotyping and standardized 12-loci-MIRU-VNTR method. The results showed Beijing/Beijing-like family was the major genotype of MTB in the northern (58%), eastern (53%), and southern (33%) regions. The second most widely spread lineage were the EAI-Manila (20% in the west and south) and Haarlem family (13-27% in the south, west, and east). According to the cluster analysis of 12-MIRU-VNTR genotypes, there were differences in distribution of MTB genotype between the northern and southern regions, and a temporal relationship between isolation year and 12-MIRU-VNTR genotype especially in loci 26 and 39 might exist. Furthermore, some patients with cavity lesions on chest films were associated with a cluster of Beijing family MTB strains, which can be defined by cluster analysis of 12-MIRU-VNTR genotype. However, the results of 12-loci-MIRU-VNTR genotyping in a longitudinal study should be interpreted with caution due to its short term instability. Further investigations of different molecular methodologies are necessary.

Original languageEnglish
Pages (from-to)28-37
Number of pages10
JournalInfection, Genetics and Evolution
Volume12
Issue number1
DOIs
Publication statusPublished - Jan 2012

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tuberculosis
Mycobacterium tuberculosis
Taiwan
genotype
Genotype
minisatellite repeats
Minisatellite Repeats
Cluster Analysis
China
cluster analysis
Multidrug-Resistant Tuberculosis
molecular epidemiology
Molecular Epidemiology
population migration
molecular dynamics
chest
Temporal Lobe
Molecular Dynamics Simulation
Motion Pictures
longitudinal studies

Keywords

  • MIRU-VNTR
  • Molecular epidemiology
  • Mycobacterium tuberculosis
  • Spoligotyping
  • Taiwan

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Association of Mycobacterium tuberculosis genotypes and clinical and epidemiological features - A multi-center study in Taiwan. / Huang, Shiang Fen; Su, Wei Juin; Dou, Horng Yunn; Feng, Jia Yih; Lee, Yu Chin; Huang, Ruay Ming; Lin, Ching Hsiung; Hwang, Jhi Jhu; Lee, Jen Jyh; Yu, Ming Chih.

In: Infection, Genetics and Evolution, Vol. 12, No. 1, 01.2012, p. 28-37.

Research output: Contribution to journalArticle

Huang, Shiang Fen ; Su, Wei Juin ; Dou, Horng Yunn ; Feng, Jia Yih ; Lee, Yu Chin ; Huang, Ruay Ming ; Lin, Ching Hsiung ; Hwang, Jhi Jhu ; Lee, Jen Jyh ; Yu, Ming Chih. / Association of Mycobacterium tuberculosis genotypes and clinical and epidemiological features - A multi-center study in Taiwan. In: Infection, Genetics and Evolution. 2012 ; Vol. 12, No. 1. pp. 28-37.
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abstract = "Genotypes of Mycobacterium tuberculosis (MTB) are related to the geographic origin of the patients and population migration. The relationship between genotypes of MTB and clinical presentations has mainly focused on transmission of multi-drug resistant MTB strain in population. This study aimed to investigate the molecular epidemiology and dynamic change of MTB genotypes in Taiwan, and their association with clinical presentation among patients with pulmonary tuberculosis. A multi-center, two-year study which enrolled 516 patients with 516 MTB isolates was conducted, including: (1) 254 isolates from northern Taiwan; (2) 38 isolates from mid-western Taiwan; (3) 211 isolates from southern Taiwan; and (4) 13 isolates from the east coast of Taiwan. The isolates were genotyped with spoligotyping and standardized 12-loci-MIRU-VNTR method. The results showed Beijing/Beijing-like family was the major genotype of MTB in the northern (58{\%}), eastern (53{\%}), and southern (33{\%}) regions. The second most widely spread lineage were the EAI-Manila (20{\%} in the west and south) and Haarlem family (13-27{\%} in the south, west, and east). According to the cluster analysis of 12-MIRU-VNTR genotypes, there were differences in distribution of MTB genotype between the northern and southern regions, and a temporal relationship between isolation year and 12-MIRU-VNTR genotype especially in loci 26 and 39 might exist. Furthermore, some patients with cavity lesions on chest films were associated with a cluster of Beijing family MTB strains, which can be defined by cluster analysis of 12-MIRU-VNTR genotype. However, the results of 12-loci-MIRU-VNTR genotyping in a longitudinal study should be interpreted with caution due to its short term instability. Further investigations of different molecular methodologies are necessary.",
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