Association of IS605 and cag -PAI of helicobacter pylori isolated from patients with gastrointestinal diseases in Taiwan

Chih Ho Lai, Chin Lin Perng, Keng Hsin Lan, Hwai Jeng Lin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background. The cag pathogenicity island (cag-PAI) is one of the most important virulent determinants of Helicobacter pylori. An insertion sequence (IS) element of cag-PAI (IS605) has been found to generate H. pylori strains with varying virulence. Aim. To evaluate the impact of IS605 and cag-PAI on H. pylori strains isolated from Taiwanese patients with severity of gastric diseases. Methods. H. pylori isolates were cultured from gastric biopsies from 99 patients with peptic ulcer, chronic gastritis, and gastric carcinoma. Six distinct, well-separated colonies were isolated from each patient and analyzed by genotyping. Results. cagA, cagE, cagM, cagT, orf10, and orf13 were found to be present in 90.0%-100.0% of the H. pylori isolates. A total deletion of cagA, cagE, cagM, cagT, orf10, and orf13 was found in 1 isolate (1.0%). The IS605 element was found to be positive in 15.2% of the isolates. The presence of IS605 was higher in H. pylori isolated from patients with gastric carcinoma (25.0%) than in patients with duodenal ulcer (6.5%) or chronic gastritis (6.3%) (P

Original languageEnglish
Article number356217
JournalGastroenterology Research and Practice
Volume2013
DOIs
Publication statusPublished - 2013

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Gastrointestinal Diseases
Taiwan
Helicobacter pylori
Genomic Islands
Stomach
Gastritis
Carcinoma
Stomach Diseases
DNA Transposable Elements
Duodenal Ulcer
Peptic Ulcer
Virulence
Biopsy

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Association of IS605 and cag -PAI of helicobacter pylori isolated from patients with gastrointestinal diseases in Taiwan. / Lai, Chih Ho; Perng, Chin Lin; Lan, Keng Hsin; Lin, Hwai Jeng.

In: Gastroenterology Research and Practice, Vol. 2013, 356217, 2013.

Research output: Contribution to journalArticle

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abstract = "Background. The cag pathogenicity island (cag-PAI) is one of the most important virulent determinants of Helicobacter pylori. An insertion sequence (IS) element of cag-PAI (IS605) has been found to generate H. pylori strains with varying virulence. Aim. To evaluate the impact of IS605 and cag-PAI on H. pylori strains isolated from Taiwanese patients with severity of gastric diseases. Methods. H. pylori isolates were cultured from gastric biopsies from 99 patients with peptic ulcer, chronic gastritis, and gastric carcinoma. Six distinct, well-separated colonies were isolated from each patient and analyzed by genotyping. Results. cagA, cagE, cagM, cagT, orf10, and orf13 were found to be present in 90.0{\%}-100.0{\%} of the H. pylori isolates. A total deletion of cagA, cagE, cagM, cagT, orf10, and orf13 was found in 1 isolate (1.0{\%}). The IS605 element was found to be positive in 15.2{\%} of the isolates. The presence of IS605 was higher in H. pylori isolated from patients with gastric carcinoma (25.0{\%}) than in patients with duodenal ulcer (6.5{\%}) or chronic gastritis (6.3{\%}) (P",
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AU - Perng, Chin Lin

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AU - Lin, Hwai Jeng

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