Abstract
Objective. The interleukin-18 (IL-18) encoding gene has three common single-nucleotide polymorphisms at 607C/A, 137G/C and 105A/C, which have been reported to be associated with several diseases. The aim of this study is to test whether IL-18 polymorphisms could act as genetic markers for renal stone disease. Material and methods. A control group of 104 healthy subjects, and 272 patients with recurrent calcium oxalate stones were examined. Polymerase chain reaction-based restriction endonuclease analysis was used to detect IL-18 polymorphisms. Results. The patient and control groups differed significantly in genotypic expression of the IL-18 105A/C polymorphism. The prevalence of the A/C C/C genotypes in the patients was higher than that in the controls. The allelic frequency of IL-18 105A/C differed significantly between the patients and the controls. The odds ratio (OR) of the A/C heterozygote of IL-18 105A/C associated with urolithiasis was 2.772. The OR of the A/C C/C genotypes of IL-18 105A/C associated with urolithiasis was 3.097. The OR per copy of the C allele of IL-18 105A/C associated with urolithiasis was 4.143. There were also significant differences in the prevalence of genotype IL-18 137G/C polymorphisms between the patients and controls. The distribution of the G/G homozygote in the patients was higher than that in the controls. There was no significant difference in genotype and allelic frequency at the IL-18 607C/A polymorphism between patients and control subjects. Conclusion. The results indicate that IL-18 105A/C polymorphisms may play a role in the development of urolithiasis.
| Original language | English |
|---|---|
| Pages (from-to) | 20-26 |
| Number of pages | 7 |
| Journal | Scandinavian Journal of Urology and Nephrology |
| Volume | 44 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2010 |
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Keywords
- Calcium oxalate
- Interleukin-18 gene
- Single-nucleotide polymorphisms
- Urolithiasis
ASJC Scopus subject areas
- Nephrology
- Urology
Cite this
Association of interleukin-18 gene polymorphisms with calcium oxalate kidney stone disease. / Lai, Kuang Chi; Lin, Wei Yong; Man, Kee Ming; Tsai, Chou Haung; Chen, Huey Yi; Tsai, Fuu Jen; Chen, Fun Jou; Chen, Hsin Yi; Liu, Hsin Ping; Ho, Tsung Jung; Huang, Po Hsun; Liu, Po Len; Lin, Feng Yen; Shen, Jui Lung; Liu, Jung Tung; Chen, Yung Hsiang; Chen, Wen Chi.
In: Scandinavian Journal of Urology and Nephrology, Vol. 44, No. 1, 2010, p. 20-26.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of interleukin-18 gene polymorphisms with calcium oxalate kidney stone disease
AU - Lai, Kuang Chi
AU - Lin, Wei Yong
AU - Man, Kee Ming
AU - Tsai, Chou Haung
AU - Chen, Huey Yi
AU - Tsai, Fuu Jen
AU - Chen, Fun Jou
AU - Chen, Hsin Yi
AU - Liu, Hsin Ping
AU - Ho, Tsung Jung
AU - Huang, Po Hsun
AU - Liu, Po Len
AU - Lin, Feng Yen
AU - Shen, Jui Lung
AU - Liu, Jung Tung
AU - Chen, Yung Hsiang
AU - Chen, Wen Chi
PY - 2010
Y1 - 2010
N2 - Objective. The interleukin-18 (IL-18) encoding gene has three common single-nucleotide polymorphisms at 607C/A, 137G/C and 105A/C, which have been reported to be associated with several diseases. The aim of this study is to test whether IL-18 polymorphisms could act as genetic markers for renal stone disease. Material and methods. A control group of 104 healthy subjects, and 272 patients with recurrent calcium oxalate stones were examined. Polymerase chain reaction-based restriction endonuclease analysis was used to detect IL-18 polymorphisms. Results. The patient and control groups differed significantly in genotypic expression of the IL-18 105A/C polymorphism. The prevalence of the A/C C/C genotypes in the patients was higher than that in the controls. The allelic frequency of IL-18 105A/C differed significantly between the patients and the controls. The odds ratio (OR) of the A/C heterozygote of IL-18 105A/C associated with urolithiasis was 2.772. The OR of the A/C C/C genotypes of IL-18 105A/C associated with urolithiasis was 3.097. The OR per copy of the C allele of IL-18 105A/C associated with urolithiasis was 4.143. There were also significant differences in the prevalence of genotype IL-18 137G/C polymorphisms between the patients and controls. The distribution of the G/G homozygote in the patients was higher than that in the controls. There was no significant difference in genotype and allelic frequency at the IL-18 607C/A polymorphism between patients and control subjects. Conclusion. The results indicate that IL-18 105A/C polymorphisms may play a role in the development of urolithiasis.
AB - Objective. The interleukin-18 (IL-18) encoding gene has three common single-nucleotide polymorphisms at 607C/A, 137G/C and 105A/C, which have been reported to be associated with several diseases. The aim of this study is to test whether IL-18 polymorphisms could act as genetic markers for renal stone disease. Material and methods. A control group of 104 healthy subjects, and 272 patients with recurrent calcium oxalate stones were examined. Polymerase chain reaction-based restriction endonuclease analysis was used to detect IL-18 polymorphisms. Results. The patient and control groups differed significantly in genotypic expression of the IL-18 105A/C polymorphism. The prevalence of the A/C C/C genotypes in the patients was higher than that in the controls. The allelic frequency of IL-18 105A/C differed significantly between the patients and the controls. The odds ratio (OR) of the A/C heterozygote of IL-18 105A/C associated with urolithiasis was 2.772. The OR of the A/C C/C genotypes of IL-18 105A/C associated with urolithiasis was 3.097. The OR per copy of the C allele of IL-18 105A/C associated with urolithiasis was 4.143. There were also significant differences in the prevalence of genotype IL-18 137G/C polymorphisms between the patients and controls. The distribution of the G/G homozygote in the patients was higher than that in the controls. There was no significant difference in genotype and allelic frequency at the IL-18 607C/A polymorphism between patients and control subjects. Conclusion. The results indicate that IL-18 105A/C polymorphisms may play a role in the development of urolithiasis.
KW - Calcium oxalate
KW - Interleukin-18 gene
KW - Single-nucleotide polymorphisms
KW - Urolithiasis
UR - http://www.scopus.com/inward/record.url?scp=76749163285&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76749163285&partnerID=8YFLogxK
U2 - 10.3109/00365590903449332
DO - 10.3109/00365590903449332
M3 - Article
C2 - 20017708
AN - SCOPUS:76749163285
VL - 44
SP - 20
EP - 26
JO - Scandinavian Journal of Urology
JF - Scandinavian Journal of Urology
SN - 2168-1805
IS - 1
ER -