Association of globular β-actin with intracellular lipid droplets in rat adrenocortical cells and adipocytes

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Abstract

Proteins located on the surface of lipid droplets may mediate intracellular lipid metabolism. In the present study, immunofluorescent staining and polyacrylamide gel electrophoresis demonstrated that actin (43 kD) is associated with isolated intracellular lipid droplets of rat adrenocortical cells and adipocytes. Two-dimensional gel electrophoresis and immunoblot analysis further confirmed that the lipid droplet-associated actin is the beta isoform. In cultured adrenocortical cells, stress fibers and the surface of intracellular lipid droplets were labeled with anti-beta-actin monoclonal antibody, whereas FITC-phalloidin staining did not mark the rim of lipid droplets. The present results provide the first morphological evidence that globular beta-actin is associated with intracellular lipid droplets. This significant association of actin with the surface of lipid droplets suggests that beta-actin might be involved in the regulation of intracellular lipid metabolism, particularly providing insight into the important transport of lipid constituents.

Original languageEnglish
Pages (from-to)1168-1174
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume289
Issue number5
DOIs
Publication statusPublished - Dec 21 2001

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Adipocytes
Rats
Actins
Lipids
Lipid Metabolism
Electrophoresis
Staining and Labeling
Phalloidine
Stress Fibers
Fluorescein-5-isothiocyanate
Electrophoresis, Gel, Two-Dimensional
Lipid Droplets
Polyacrylamide Gel Electrophoresis
Cultured Cells
Protein Isoforms
Monoclonal Antibodies
Gels
Association reactions
Fibers
Proteins

Keywords

  • β-actin
  • Adipocytes
  • Adrenocortical cells
  • Lipid droplet

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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title = "Association of globular β-actin with intracellular lipid droplets in rat adrenocortical cells and adipocytes",
abstract = "Proteins located on the surface of lipid droplets may mediate intracellular lipid metabolism. In the present study, immunofluorescent staining and polyacrylamide gel electrophoresis demonstrated that actin (43 kD) is associated with isolated intracellular lipid droplets of rat adrenocortical cells and adipocytes. Two-dimensional gel electrophoresis and immunoblot analysis further confirmed that the lipid droplet-associated actin is the beta isoform. In cultured adrenocortical cells, stress fibers and the surface of intracellular lipid droplets were labeled with anti-beta-actin monoclonal antibody, whereas FITC-phalloidin staining did not mark the rim of lipid droplets. The present results provide the first morphological evidence that globular beta-actin is associated with intracellular lipid droplets. This significant association of actin with the surface of lipid droplets suggests that beta-actin might be involved in the regulation of intracellular lipid metabolism, particularly providing insight into the important transport of lipid constituents.",
keywords = "β-actin, Adipocytes, Adrenocortical cells, Lipid droplet",
author = "Fong, {Tsorng Harn} and Wu, {Ching Hsiang} and Liao, {E. Wen} and Chang, {Chiu Yun} and Pai, {Man Hui} and Chiou, {Ruei Jen} and Lee, {Ai Wei}",
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TY - JOUR

T1 - Association of globular β-actin with intracellular lipid droplets in rat adrenocortical cells and adipocytes

AU - Fong, Tsorng Harn

AU - Wu, Ching Hsiang

AU - Liao, E. Wen

AU - Chang, Chiu Yun

AU - Pai, Man Hui

AU - Chiou, Ruei Jen

AU - Lee, Ai Wei

PY - 2001/12/21

Y1 - 2001/12/21

N2 - Proteins located on the surface of lipid droplets may mediate intracellular lipid metabolism. In the present study, immunofluorescent staining and polyacrylamide gel electrophoresis demonstrated that actin (43 kD) is associated with isolated intracellular lipid droplets of rat adrenocortical cells and adipocytes. Two-dimensional gel electrophoresis and immunoblot analysis further confirmed that the lipid droplet-associated actin is the beta isoform. In cultured adrenocortical cells, stress fibers and the surface of intracellular lipid droplets were labeled with anti-beta-actin monoclonal antibody, whereas FITC-phalloidin staining did not mark the rim of lipid droplets. The present results provide the first morphological evidence that globular beta-actin is associated with intracellular lipid droplets. This significant association of actin with the surface of lipid droplets suggests that beta-actin might be involved in the regulation of intracellular lipid metabolism, particularly providing insight into the important transport of lipid constituents.

AB - Proteins located on the surface of lipid droplets may mediate intracellular lipid metabolism. In the present study, immunofluorescent staining and polyacrylamide gel electrophoresis demonstrated that actin (43 kD) is associated with isolated intracellular lipid droplets of rat adrenocortical cells and adipocytes. Two-dimensional gel electrophoresis and immunoblot analysis further confirmed that the lipid droplet-associated actin is the beta isoform. In cultured adrenocortical cells, stress fibers and the surface of intracellular lipid droplets were labeled with anti-beta-actin monoclonal antibody, whereas FITC-phalloidin staining did not mark the rim of lipid droplets. The present results provide the first morphological evidence that globular beta-actin is associated with intracellular lipid droplets. This significant association of actin with the surface of lipid droplets suggests that beta-actin might be involved in the regulation of intracellular lipid metabolism, particularly providing insight into the important transport of lipid constituents.

KW - β-actin

KW - Adipocytes

KW - Adrenocortical cells

KW - Lipid droplet

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U2 - 10.1006/bbrc.2001.6080

DO - 10.1006/bbrc.2001.6080

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JF - Biochemical and Biophysical Research Communications

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