Abstract
The estrogen receptor ± gene (ESR1) is an important mediator of the atheroprotective effect of estrogen on the vasculature system. We examined the potential associations between common single nucleotide polymorphism (SNP) variants of ESR1 and intima-media thickness (IMT) in carotid arteries, a strong predictor of cardiovascular disease (CVD). A total of 760 study participants (343 men and 407 women), who had undergone a Duplex ultrasonographic examination of carotid artery, were investigated. Measurement of IMT was performed on a 10-mm segment of the common carotid artery (CCA). Fourteen sequence-validated SNPs of high frequency of Oriental origin were selected and genotyped by the method of Light-Cycler-480-assisted real-time polymerase chain reaction (PCR) followed by melting curve analysis. Results from multiple linear regression analyses showed significant associations of SNPs rs2228480 (Ex8+229G>A) and rs3798758 (Ex8+1988C>A) with the carotid IMT values in women but not in men. Women with SNP rs2228480 (Ex8+229G>A) A/A genotype had a 0.048 mm (7.1%) increase in IMT values versus the other genotypes combined (P =.030). In women who carried the rs3798758 (Ex8+1988C>A) CA+AA combined genotypes, their carotid IMT measures were 0.020 mm (2.9%) decreased as compared with those in women who carried C/ C genotype (P =.042). In haplotype analysis, women with the T-A haplotype versus C-C haplotype of combined rs3798577 (Ex8+1264T>C) and rs3798758 (Ex8+1988C>A) were also found to be associated with a decreased IMT value at a borderline significance (P =.057). Some common SNPs in the ESR1 could be important in modulating carotid atherosclerosis and thereby CVD susceptibility in Taiwanese women.
| Original language | English |
|---|---|
| Pages (from-to) | 275-282 |
| Number of pages | 8 |
| Journal | Angiology |
| Volume | 61 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 2010 |
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Keywords
- Association
- Epidemiology
- Estrogen receptor ±
- Genetics
- Intima-media thickness
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Association of estrogen receptor ± genotypes/ haplotypes with carotid intima-media thickness in taiwanese women. / Wu, Meei Maan; Hsieh, Yi Chen; Lien, Li Ming; Chen, Wei Hung; Bai, Chyi Huey; Chiu, Hou Chang; Chen, Hsin Hon; Chung, Wen Tin; Lee, Yuan Chii; Hsu, Chung Y.; Lin, Hui Wen; Chiou, Hung Yi.
In: Angiology, Vol. 61, No. 3, 06.2010, p. 275-282.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of estrogen receptor ± genotypes/ haplotypes with carotid intima-media thickness in taiwanese women
AU - Wu, Meei Maan
AU - Hsieh, Yi Chen
AU - Lien, Li Ming
AU - Chen, Wei Hung
AU - Bai, Chyi Huey
AU - Chiu, Hou Chang
AU - Chen, Hsin Hon
AU - Chung, Wen Tin
AU - Lee, Yuan Chii
AU - Hsu, Chung Y.
AU - Lin, Hui Wen
AU - Chiou, Hung Yi
PY - 2010/6
Y1 - 2010/6
N2 - The estrogen receptor ± gene (ESR1) is an important mediator of the atheroprotective effect of estrogen on the vasculature system. We examined the potential associations between common single nucleotide polymorphism (SNP) variants of ESR1 and intima-media thickness (IMT) in carotid arteries, a strong predictor of cardiovascular disease (CVD). A total of 760 study participants (343 men and 407 women), who had undergone a Duplex ultrasonographic examination of carotid artery, were investigated. Measurement of IMT was performed on a 10-mm segment of the common carotid artery (CCA). Fourteen sequence-validated SNPs of high frequency of Oriental origin were selected and genotyped by the method of Light-Cycler-480-assisted real-time polymerase chain reaction (PCR) followed by melting curve analysis. Results from multiple linear regression analyses showed significant associations of SNPs rs2228480 (Ex8+229G>A) and rs3798758 (Ex8+1988C>A) with the carotid IMT values in women but not in men. Women with SNP rs2228480 (Ex8+229G>A) A/A genotype had a 0.048 mm (7.1%) increase in IMT values versus the other genotypes combined (P =.030). In women who carried the rs3798758 (Ex8+1988C>A) CA+AA combined genotypes, their carotid IMT measures were 0.020 mm (2.9%) decreased as compared with those in women who carried C/ C genotype (P =.042). In haplotype analysis, women with the T-A haplotype versus C-C haplotype of combined rs3798577 (Ex8+1264T>C) and rs3798758 (Ex8+1988C>A) were also found to be associated with a decreased IMT value at a borderline significance (P =.057). Some common SNPs in the ESR1 could be important in modulating carotid atherosclerosis and thereby CVD susceptibility in Taiwanese women.
AB - The estrogen receptor ± gene (ESR1) is an important mediator of the atheroprotective effect of estrogen on the vasculature system. We examined the potential associations between common single nucleotide polymorphism (SNP) variants of ESR1 and intima-media thickness (IMT) in carotid arteries, a strong predictor of cardiovascular disease (CVD). A total of 760 study participants (343 men and 407 women), who had undergone a Duplex ultrasonographic examination of carotid artery, were investigated. Measurement of IMT was performed on a 10-mm segment of the common carotid artery (CCA). Fourteen sequence-validated SNPs of high frequency of Oriental origin were selected and genotyped by the method of Light-Cycler-480-assisted real-time polymerase chain reaction (PCR) followed by melting curve analysis. Results from multiple linear regression analyses showed significant associations of SNPs rs2228480 (Ex8+229G>A) and rs3798758 (Ex8+1988C>A) with the carotid IMT values in women but not in men. Women with SNP rs2228480 (Ex8+229G>A) A/A genotype had a 0.048 mm (7.1%) increase in IMT values versus the other genotypes combined (P =.030). In women who carried the rs3798758 (Ex8+1988C>A) CA+AA combined genotypes, their carotid IMT measures were 0.020 mm (2.9%) decreased as compared with those in women who carried C/ C genotype (P =.042). In haplotype analysis, women with the T-A haplotype versus C-C haplotype of combined rs3798577 (Ex8+1264T>C) and rs3798758 (Ex8+1988C>A) were also found to be associated with a decreased IMT value at a borderline significance (P =.057). Some common SNPs in the ESR1 could be important in modulating carotid atherosclerosis and thereby CVD susceptibility in Taiwanese women.
KW - Association
KW - Epidemiology
KW - Estrogen receptor ±
KW - Genetics
KW - Intima-media thickness
KW - Association
KW - Epidemiology
KW - Estrogen receptor ±
KW - Genetics
KW - Intima-media thickness
UR - http://www.scopus.com/inward/record.url?scp=77949878773&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77949878773&partnerID=8YFLogxK
U2 - 10.1177/0003319709351256
DO - 10.1177/0003319709351256
M3 - Article
VL - 61
SP - 275
EP - 282
JO - Angiology
JF - Angiology
SN - 0003-3197
IS - 3
ER -