Association of cooking oil fumes exposure with lung cancer: Involvement of inhibitor of apoptosis proteins in cell survival and proliferation in vitro

Huey Shan Hung, Wen Jun Wu, Ya Wen Cheng, Tsu Chin Wu, Kee Lung Chang, Huei Lee

Research output: Contribution to journalArticle

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Abstract

Cooking oil fumes (COF) have been shown to be associated with lung cancer incidence in Chinese women. Our recent report indicates that inhibitor of apoptosis protein 2 (IAP2) induced by COF may contribute to the survival and proliferation of A549 lung cancer cells. In this study, to further verify whether other antiapoptosis proteins including IAP1, X-linked IAP (XIAP), and survivin, were linked with lung cancer cell survival and proliferation, these IAPs expressions in A549 cells after treatment with COF and its two major components, benzo[a]pyrene (BaP) and 2,4-decadienal (2,4-DDE) were evaluated by Western blotting. Our data showed that IAP2 was significantly induced by COF, BaP, and 2,4-DDE, but XIAP was decreased by COF and 2,4-DDE, but not by BaP. Even though different effects of COF and 2,4-DDE on IAP2 and XIAP protein expressions were observed, the caspase-3 expression was diminished by COF and 2,4-DDE. In addition, induction of IAP2 and phosphorylated Akt proteins by COF and 2,4-DDE were simultaneously abolished by LY294002. Flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) analysis showed that the proportion of A549 cells at the S-phase was increased significantly after treatment with COF or 2,4-DDE. The cell proliferation induced by COF is associated with the attenuation of p21Cip/Waf1 expression. Therefore, increases of IAP1, IAP2, survivin, and cyclin D1 expressions and decreases of XIAP, caspase-3, and p21 expressions might partly contribute to the survival and proliferation of lung cancer cells after exposure to 2,4-DDE and COF. In conclusion, the lung cancer cell growth promoted by COF might support previous epidemiological reports indicating that exposure of COF was associated with lung cancer development among Chinese women.

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume628
Issue number2
DOIs
Publication statusPublished - Apr 2 2007
Externally publishedYes

Fingerprint

Inhibitor of Apoptosis Proteins
Cooking
Lung Neoplasms
Cell Survival
Oils
Cell Proliferation
Caspase 3
In Vitro Techniques
X-Linked Inhibitor of Apoptosis Protein
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Survival
DNA Nucleotidylexotransferase
2,4-decadienal
Benzo(a)pyrene
Cyclin D1
S Phase

Keywords

  • 2,4-DDE
  • Cell survival and proliferation
  • Cooking oil fumes (COF)
  • Inhibitor of apoptosis proteins (IAPs)
  • Lung cancer

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Genetics

Cite this

Association of cooking oil fumes exposure with lung cancer : Involvement of inhibitor of apoptosis proteins in cell survival and proliferation in vitro. / Hung, Huey Shan; Wu, Wen Jun; Cheng, Ya Wen; Wu, Tsu Chin; Chang, Kee Lung; Lee, Huei.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 628, No. 2, 02.04.2007, p. 107-116.

Research output: Contribution to journalArticle

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AU - Chang, Kee Lung

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AB - Cooking oil fumes (COF) have been shown to be associated with lung cancer incidence in Chinese women. Our recent report indicates that inhibitor of apoptosis protein 2 (IAP2) induced by COF may contribute to the survival and proliferation of A549 lung cancer cells. In this study, to further verify whether other antiapoptosis proteins including IAP1, X-linked IAP (XIAP), and survivin, were linked with lung cancer cell survival and proliferation, these IAPs expressions in A549 cells after treatment with COF and its two major components, benzo[a]pyrene (BaP) and 2,4-decadienal (2,4-DDE) were evaluated by Western blotting. Our data showed that IAP2 was significantly induced by COF, BaP, and 2,4-DDE, but XIAP was decreased by COF and 2,4-DDE, but not by BaP. Even though different effects of COF and 2,4-DDE on IAP2 and XIAP protein expressions were observed, the caspase-3 expression was diminished by COF and 2,4-DDE. In addition, induction of IAP2 and phosphorylated Akt proteins by COF and 2,4-DDE were simultaneously abolished by LY294002. Flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) analysis showed that the proportion of A549 cells at the S-phase was increased significantly after treatment with COF or 2,4-DDE. The cell proliferation induced by COF is associated with the attenuation of p21Cip/Waf1 expression. Therefore, increases of IAP1, IAP2, survivin, and cyclin D1 expressions and decreases of XIAP, caspase-3, and p21 expressions might partly contribute to the survival and proliferation of lung cancer cells after exposure to 2,4-DDE and COF. In conclusion, the lung cancer cell growth promoted by COF might support previous epidemiological reports indicating that exposure of COF was associated with lung cancer development among Chinese women.

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