Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk

Hsiu Ting Tsai, Ching Hu Wu, Hsiao Ling Lai, Ruei Nian Li, Yi Ching Tung, Hung Yi Chuang, Trong Neng Wu, Li Jen Lin, Chi Kung Ho, Hon Wein Liu, Ming Tsang Wu

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Human papillomavirus (HPV) infection is a high-risk factor for cervical intraepithelial neoplasm (CIN) but the association between the quantitative HPV DNA load and the severity of CIN remains controversial. We conducted a community study to investigate the correlation between the two. Potential study subjects were selected through Pap smear screening in Kaohsiung County, Taiwan. Ninety-one subjects with either their first case of inflammation or ≥CIN1 by biopsy confirmation were assigned to a case group; 175 normal subjects with negative findings by Pap smears or biopsies were assigned to a control group. Cervical HPV load was detected with Hybrid Capture II assay for high-risk HPV infection, with nested PCR for high- and low-risk HPV infection, and with type-specific PCR for HPV type 16 (HPV-16). Individuals with positive high-risk HPV infection had an increased risk of developing CIN. Compared with HPV-negative subjects, the odds ratios were 32.2 [95% confidence interval (95% CI), 10.4-99.5] for subjects with CIN1, 37.2 (95% CI, 7.4-187.6) for subjects with CIN2, and 68.3 (95% CI, 14.1-328.5) for subjects with ≥CIN3 after adjusting for other confounding factors. The similar trend was also found among the HPV-16-negative individuals. In addition, high-risk HPV DNA load levels were highly correlated with the different grades of CINs in the overall population (Spearman's correlation coefficient r = 0.67, P <0.0001, n = 266) and the HPV-16-negative population (Spearman's correlation coefficient r = 0.58, P <0.0001, n = 234). We concluded that high-risk HPV infection, irrespective of HPV-16 infection, was highly and positively associated with the development of CIN.

Original languageEnglish
Pages (from-to)2544-2549
Number of pages6
JournalCancer Epidemiology Biomarkers and Prevention
Volume14
Issue number11 I
DOIs
Publication statusPublished - Nov 2005
Externally publishedYes

Fingerprint

Cervical Intraepithelial Neoplasia
Papillomavirus Infections
DNA
Human papillomavirus 16
Papanicolaou Test
Confidence Intervals
Biopsy
Polymerase Chain Reaction
Taiwan
Population
Odds Ratio
Inflammation
Control Groups

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk. / Tsai, Hsiu Ting; Wu, Ching Hu; Lai, Hsiao Ling; Li, Ruei Nian; Tung, Yi Ching; Chuang, Hung Yi; Wu, Trong Neng; Lin, Li Jen; Ho, Chi Kung; Liu, Hon Wein; Wu, Ming Tsang.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 14, No. 11 I, 11.2005, p. 2544-2549.

Research output: Contribution to journalArticle

Tsai, Hsiu Ting ; Wu, Ching Hu ; Lai, Hsiao Ling ; Li, Ruei Nian ; Tung, Yi Ching ; Chuang, Hung Yi ; Wu, Trong Neng ; Lin, Li Jen ; Ho, Chi Kung ; Liu, Hon Wein ; Wu, Ming Tsang. / Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk. In: Cancer Epidemiology Biomarkers and Prevention. 2005 ; Vol. 14, No. 11 I. pp. 2544-2549.
@article{b8e61dc955bb43eaad667757cddaeec3,
title = "Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk",
abstract = "Human papillomavirus (HPV) infection is a high-risk factor for cervical intraepithelial neoplasm (CIN) but the association between the quantitative HPV DNA load and the severity of CIN remains controversial. We conducted a community study to investigate the correlation between the two. Potential study subjects were selected through Pap smear screening in Kaohsiung County, Taiwan. Ninety-one subjects with either their first case of inflammation or ≥CIN1 by biopsy confirmation were assigned to a case group; 175 normal subjects with negative findings by Pap smears or biopsies were assigned to a control group. Cervical HPV load was detected with Hybrid Capture II assay for high-risk HPV infection, with nested PCR for high- and low-risk HPV infection, and with type-specific PCR for HPV type 16 (HPV-16). Individuals with positive high-risk HPV infection had an increased risk of developing CIN. Compared with HPV-negative subjects, the odds ratios were 32.2 [95{\%} confidence interval (95{\%} CI), 10.4-99.5] for subjects with CIN1, 37.2 (95{\%} CI, 7.4-187.6) for subjects with CIN2, and 68.3 (95{\%} CI, 14.1-328.5) for subjects with ≥CIN3 after adjusting for other confounding factors. The similar trend was also found among the HPV-16-negative individuals. In addition, high-risk HPV DNA load levels were highly correlated with the different grades of CINs in the overall population (Spearman's correlation coefficient r = 0.67, P <0.0001, n = 266) and the HPV-16-negative population (Spearman's correlation coefficient r = 0.58, P <0.0001, n = 234). We concluded that high-risk HPV infection, irrespective of HPV-16 infection, was highly and positively associated with the development of CIN.",
author = "Tsai, {Hsiu Ting} and Wu, {Ching Hu} and Lai, {Hsiao Ling} and Li, {Ruei Nian} and Tung, {Yi Ching} and Chuang, {Hung Yi} and Wu, {Trong Neng} and Lin, {Li Jen} and Ho, {Chi Kung} and Liu, {Hon Wein} and Wu, {Ming Tsang}",
year = "2005",
month = "11",
doi = "10.1158/1055-9965.EPI-05-0240",
language = "English",
volume = "14",
pages = "2544--2549",
journal = "Cancer Epidemiology Biomarkers and Prevention",
issn = "1055-9965",
publisher = "American Association for Cancer Research Inc.",
number = "11 I",

}

TY - JOUR

T1 - Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk

AU - Tsai, Hsiu Ting

AU - Wu, Ching Hu

AU - Lai, Hsiao Ling

AU - Li, Ruei Nian

AU - Tung, Yi Ching

AU - Chuang, Hung Yi

AU - Wu, Trong Neng

AU - Lin, Li Jen

AU - Ho, Chi Kung

AU - Liu, Hon Wein

AU - Wu, Ming Tsang

PY - 2005/11

Y1 - 2005/11

N2 - Human papillomavirus (HPV) infection is a high-risk factor for cervical intraepithelial neoplasm (CIN) but the association between the quantitative HPV DNA load and the severity of CIN remains controversial. We conducted a community study to investigate the correlation between the two. Potential study subjects were selected through Pap smear screening in Kaohsiung County, Taiwan. Ninety-one subjects with either their first case of inflammation or ≥CIN1 by biopsy confirmation were assigned to a case group; 175 normal subjects with negative findings by Pap smears or biopsies were assigned to a control group. Cervical HPV load was detected with Hybrid Capture II assay for high-risk HPV infection, with nested PCR for high- and low-risk HPV infection, and with type-specific PCR for HPV type 16 (HPV-16). Individuals with positive high-risk HPV infection had an increased risk of developing CIN. Compared with HPV-negative subjects, the odds ratios were 32.2 [95% confidence interval (95% CI), 10.4-99.5] for subjects with CIN1, 37.2 (95% CI, 7.4-187.6) for subjects with CIN2, and 68.3 (95% CI, 14.1-328.5) for subjects with ≥CIN3 after adjusting for other confounding factors. The similar trend was also found among the HPV-16-negative individuals. In addition, high-risk HPV DNA load levels were highly correlated with the different grades of CINs in the overall population (Spearman's correlation coefficient r = 0.67, P <0.0001, n = 266) and the HPV-16-negative population (Spearman's correlation coefficient r = 0.58, P <0.0001, n = 234). We concluded that high-risk HPV infection, irrespective of HPV-16 infection, was highly and positively associated with the development of CIN.

AB - Human papillomavirus (HPV) infection is a high-risk factor for cervical intraepithelial neoplasm (CIN) but the association between the quantitative HPV DNA load and the severity of CIN remains controversial. We conducted a community study to investigate the correlation between the two. Potential study subjects were selected through Pap smear screening in Kaohsiung County, Taiwan. Ninety-one subjects with either their first case of inflammation or ≥CIN1 by biopsy confirmation were assigned to a case group; 175 normal subjects with negative findings by Pap smears or biopsies were assigned to a control group. Cervical HPV load was detected with Hybrid Capture II assay for high-risk HPV infection, with nested PCR for high- and low-risk HPV infection, and with type-specific PCR for HPV type 16 (HPV-16). Individuals with positive high-risk HPV infection had an increased risk of developing CIN. Compared with HPV-negative subjects, the odds ratios were 32.2 [95% confidence interval (95% CI), 10.4-99.5] for subjects with CIN1, 37.2 (95% CI, 7.4-187.6) for subjects with CIN2, and 68.3 (95% CI, 14.1-328.5) for subjects with ≥CIN3 after adjusting for other confounding factors. The similar trend was also found among the HPV-16-negative individuals. In addition, high-risk HPV DNA load levels were highly correlated with the different grades of CINs in the overall population (Spearman's correlation coefficient r = 0.67, P <0.0001, n = 266) and the HPV-16-negative population (Spearman's correlation coefficient r = 0.58, P <0.0001, n = 234). We concluded that high-risk HPV infection, irrespective of HPV-16 infection, was highly and positively associated with the development of CIN.

UR - http://www.scopus.com/inward/record.url?scp=28644447167&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28644447167&partnerID=8YFLogxK

U2 - 10.1158/1055-9965.EPI-05-0240

DO - 10.1158/1055-9965.EPI-05-0240

M3 - Article

C2 - 16284376

AN - SCOPUS:28644447167

VL - 14

SP - 2544

EP - 2549

JO - Cancer Epidemiology Biomarkers and Prevention

JF - Cancer Epidemiology Biomarkers and Prevention

SN - 1055-9965

IS - 11 I

ER -