Association between polymorphisms of ACE, B2AR, ANP and ENOS and cardiovascular diseases

A community-based study in the Matsu area

Chaur Jong Hu, Chun Hsiung Wang, Jui Heng Lee, Chih Min Hsieh, Chia Chi Cheng, Shu Chen Chang, Chee Jen Chang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Cardiovascular diseases (CVDs) are the leading cause of death in most countries of the world. In this study, associations between CVDs and polymorphisms of angiotensin-converting enzyme (ACE), atrial natriuretic peptide (ANP), β2-adrenal receptor (B2AR) and endothelial nitric oxide synthase (ENOS) genes were explored in a community-based setting. Methods: Between March and May 2001, 1740 subjects ≥35 years from the Matsu area in Taiwan were recruited to this study, representing 71.6% of the target population in Matsu. After informed consent was obtained during an interview, physical examination, resting ECG, serum biochemical profile and a questionnaire survey were used to obtain information. Genomic DNA was also collected and analyzed. Owing to technical limitations, 1186 samples were analyzed. Genetic polymorphisms of the genes in question were investigated using PCR and restriction fragment length polymorphism (RFLP). The distribution of allele frequencies for these genes was derived for stroke, coronary artery disease, hypertension, diabetes, hypercholesterolemia, hypertriglyceridemia and overweight subgroups. Results: The ENOS Glu298Asp polymorphism was associated with hypercholesterolemia (odds ratio 0.658, 95%CI 0.460-0.940; p=0.025) and the ACE D/I variant was associated with hypertriglyceridemia (odds ratio 0.722, 95%CI 0.536-0.973; p=0.033). Polymorphisms of the other genes were not associated with any of the disease groups. Conclusions: This community-based study reveals that genetic factors might play a role in the metabolism of lipids. The genetic risk for CVDs needs further investigation.

Original languageEnglish
Pages (from-to)20-25
Number of pages6
JournalClinical Chemistry and Laboratory Medicine
Volume45
Issue number1
DOIs
Publication statusPublished - Jan 1 2007

Fingerprint

Nitric Oxide Synthase Type III
Atrial Natriuretic Factor
Peptidyl-Dipeptidase A
Polymorphism
Cardiovascular Diseases
Genes
Hypertriglyceridemia
Hypercholesterolemia
Odds Ratio
Health Services Needs and Demand
Genetic Polymorphisms
Informed Consent
Taiwan
Lipid Metabolism
Gene Frequency
Restriction Fragment Length Polymorphisms
Physical Examination
Coronary Artery Disease
Cause of Death
Medical problems

Keywords

  • β-adrenal receptor (B2AR)
  • Angiotensin-converting enzyme (ACE)
  • Atrial natriuretic peptide (ANP)
  • Cardiovascular diseases
  • Community-based
  • Endothelial nitric oxide synthase (ENOS)
  • Polymorphisms

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Association between polymorphisms of ACE, B2AR, ANP and ENOS and cardiovascular diseases : A community-based study in the Matsu area. / Hu, Chaur Jong; Wang, Chun Hsiung; Lee, Jui Heng; Hsieh, Chih Min; Cheng, Chia Chi; Chang, Shu Chen; Chang, Chee Jen.

In: Clinical Chemistry and Laboratory Medicine, Vol. 45, No. 1, 01.01.2007, p. 20-25.

Research output: Contribution to journalArticle

Hu, Chaur Jong ; Wang, Chun Hsiung ; Lee, Jui Heng ; Hsieh, Chih Min ; Cheng, Chia Chi ; Chang, Shu Chen ; Chang, Chee Jen. / Association between polymorphisms of ACE, B2AR, ANP and ENOS and cardiovascular diseases : A community-based study in the Matsu area. In: Clinical Chemistry and Laboratory Medicine. 2007 ; Vol. 45, No. 1. pp. 20-25.
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abstract = "Background: Cardiovascular diseases (CVDs) are the leading cause of death in most countries of the world. In this study, associations between CVDs and polymorphisms of angiotensin-converting enzyme (ACE), atrial natriuretic peptide (ANP), β2-adrenal receptor (B2AR) and endothelial nitric oxide synthase (ENOS) genes were explored in a community-based setting. Methods: Between March and May 2001, 1740 subjects ≥35 years from the Matsu area in Taiwan were recruited to this study, representing 71.6{\%} of the target population in Matsu. After informed consent was obtained during an interview, physical examination, resting ECG, serum biochemical profile and a questionnaire survey were used to obtain information. Genomic DNA was also collected and analyzed. Owing to technical limitations, 1186 samples were analyzed. Genetic polymorphisms of the genes in question were investigated using PCR and restriction fragment length polymorphism (RFLP). The distribution of allele frequencies for these genes was derived for stroke, coronary artery disease, hypertension, diabetes, hypercholesterolemia, hypertriglyceridemia and overweight subgroups. Results: The ENOS Glu298Asp polymorphism was associated with hypercholesterolemia (odds ratio 0.658, 95{\%}CI 0.460-0.940; p=0.025) and the ACE D/I variant was associated with hypertriglyceridemia (odds ratio 0.722, 95{\%}CI 0.536-0.973; p=0.033). Polymorphisms of the other genes were not associated with any of the disease groups. Conclusions: This community-based study reveals that genetic factors might play a role in the metabolism of lipids. The genetic risk for CVDs needs further investigation.",
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T1 - Association between polymorphisms of ACE, B2AR, ANP and ENOS and cardiovascular diseases

T2 - A community-based study in the Matsu area

AU - Hu, Chaur Jong

AU - Wang, Chun Hsiung

AU - Lee, Jui Heng

AU - Hsieh, Chih Min

AU - Cheng, Chia Chi

AU - Chang, Shu Chen

AU - Chang, Chee Jen

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Background: Cardiovascular diseases (CVDs) are the leading cause of death in most countries of the world. In this study, associations between CVDs and polymorphisms of angiotensin-converting enzyme (ACE), atrial natriuretic peptide (ANP), β2-adrenal receptor (B2AR) and endothelial nitric oxide synthase (ENOS) genes were explored in a community-based setting. Methods: Between March and May 2001, 1740 subjects ≥35 years from the Matsu area in Taiwan were recruited to this study, representing 71.6% of the target population in Matsu. After informed consent was obtained during an interview, physical examination, resting ECG, serum biochemical profile and a questionnaire survey were used to obtain information. Genomic DNA was also collected and analyzed. Owing to technical limitations, 1186 samples were analyzed. Genetic polymorphisms of the genes in question were investigated using PCR and restriction fragment length polymorphism (RFLP). The distribution of allele frequencies for these genes was derived for stroke, coronary artery disease, hypertension, diabetes, hypercholesterolemia, hypertriglyceridemia and overweight subgroups. Results: The ENOS Glu298Asp polymorphism was associated with hypercholesterolemia (odds ratio 0.658, 95%CI 0.460-0.940; p=0.025) and the ACE D/I variant was associated with hypertriglyceridemia (odds ratio 0.722, 95%CI 0.536-0.973; p=0.033). Polymorphisms of the other genes were not associated with any of the disease groups. Conclusions: This community-based study reveals that genetic factors might play a role in the metabolism of lipids. The genetic risk for CVDs needs further investigation.

AB - Background: Cardiovascular diseases (CVDs) are the leading cause of death in most countries of the world. In this study, associations between CVDs and polymorphisms of angiotensin-converting enzyme (ACE), atrial natriuretic peptide (ANP), β2-adrenal receptor (B2AR) and endothelial nitric oxide synthase (ENOS) genes were explored in a community-based setting. Methods: Between March and May 2001, 1740 subjects ≥35 years from the Matsu area in Taiwan were recruited to this study, representing 71.6% of the target population in Matsu. After informed consent was obtained during an interview, physical examination, resting ECG, serum biochemical profile and a questionnaire survey were used to obtain information. Genomic DNA was also collected and analyzed. Owing to technical limitations, 1186 samples were analyzed. Genetic polymorphisms of the genes in question were investigated using PCR and restriction fragment length polymorphism (RFLP). The distribution of allele frequencies for these genes was derived for stroke, coronary artery disease, hypertension, diabetes, hypercholesterolemia, hypertriglyceridemia and overweight subgroups. Results: The ENOS Glu298Asp polymorphism was associated with hypercholesterolemia (odds ratio 0.658, 95%CI 0.460-0.940; p=0.025) and the ACE D/I variant was associated with hypertriglyceridemia (odds ratio 0.722, 95%CI 0.536-0.973; p=0.033). Polymorphisms of the other genes were not associated with any of the disease groups. Conclusions: This community-based study reveals that genetic factors might play a role in the metabolism of lipids. The genetic risk for CVDs needs further investigation.

KW - β-adrenal receptor (B2AR)

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KW - Atrial natriuretic peptide (ANP)

KW - Cardiovascular diseases

KW - Community-based

KW - Endothelial nitric oxide synthase (ENOS)

KW - Polymorphisms

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