地方性肢端壞疽症與麩胺基硫轉移酵素基因多型性關係之探討

Translated title of the contribution: Association between Glutathione S-transferase Gene Polymorphism and Endemic Gangrenous Panaritium

蔡 光昭(Kuang-Chao Tsai), Ruey-Yu Chen, 許 文憲(Wen-Haien Hsu), 陳 宗林(Tsung-Lin Chen), 葉 錦瑩(Ching-Ying Yeh)

Research output: Contribution to journalArticle

Abstract

Objectives. Since 1954, an endemic-specific spontaneous gangrene happened in the South-Western Sea Coast of Taiwan. Patients experienced numbness, cold and intermittent limping of the lower extremities, gradually symptoms getting worse, skin color of the affected parts getting darker and spreading upward, tissue necrosis and the affected foot turning into black color, which derived the name of 'Black Foot Disease, BFD'. Actually, BFO does not limited itself to lower extremities only, according to Dr. Ming-yi Wang, hands of the upper extremities are also affected, therefore 'Endemic Gangrenous Panaritium, endemic GP' is a more appropriate term. The endemic geographic cluster characteristics have received the academic attention on the arsenic and humic acid concentrations in the artesian wells of that region. But the causation has been confused, for the new cases keep sprouting even the usage of the deep wells have been ceased. Obviously, other than arsenic (As) other factors have to be investigated. Therefore a case-control study was designed to explore other risk factors apart from the outside of arsenic, antioxidant activity index and gene polymorphism re1ated with Gangrenous Panaritium.
Methods. It was a case-control study based on a total of 88 consenters (case 40, contro148). Questionnaire, blood and urine samples were collected. The biochemical examination, urinary metal levels detection and the glutathione S-transferase gene polymorphism analysis was carried out.
Results. The resu1ts showed that the case group has higher kidney function disorder index, homocysteine and metallic Pb concentrations than the control group. The results showed that GST P1 null type had no association with Gangrenous Panaritium, but proportions of GST M1 null type and GST T1 null type combination was significant1y higher in cases than control. In multiple logistic regression, after adjusted by sex and age, years resident at Black Foot area (OR=1.07 per year), GSH level (OR=0.88 per μM), and both null type in GST M1 and GST T1 (OR=49.19) were the significant risk factors, while high-fiber or seldom-salt diets might be the preventive factors.
Conclusions. The longer period of resident at Black Foot area, the higher GSH level, and both null type in GST M1 and GST T1 were the significant risk factors of endemic Gangrenous Panaritium, whi1e high-fiber or seldom-salt diets might reduce this risk.
Original languageTraditional Chinese
Pages (from-to)1-16
Number of pages16
Journal中南盟研究年刊
Volume3
Issue number1
Publication statusPublished - Mar 1 2010

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arsenic
glutathione transferase
polymorphism
risk factors
case-control studies
dietary fiber
foot diseases
salts
genes
color
homocysteine
springs (water)
renal function
humic acids
sprouting
skin (animal)
diet
signs and symptoms (animals and humans)
Taiwan
necrosis

Keywords

  • endemic gangrenous panaritium
  • Black Foot Disease BFD
  • metallic elements
  • GST polymorphism

Cite this

蔡光昭(Kuang-Chao T, Chen, R-Y., 許文憲(Wen-Haien H, 陳宗林(Tsung-Lin C, & 葉錦瑩(Ching-Ying Y (2010). 地方性肢端壞疽症與麩胺基硫轉移酵素基因多型性關係之探討. 中南盟研究年刊, 3(1), 1-16.

地方性肢端壞疽症與麩胺基硫轉移酵素基因多型性關係之探討. / 蔡光昭(Kuang-Chao Tsai); Chen, Ruey-Yu; 許文憲(Wen-Haien Hsu); 陳宗林(Tsung-Lin Chen); 葉錦瑩(Ching-Ying Yeh).

In: 中南盟研究年刊, Vol. 3, No. 1, 01.03.2010, p. 1-16.

Research output: Contribution to journalArticle

蔡光昭(Kuang-ChaoT, Chen, R-Y, 許文憲(Wen-HaienH, 陳宗林(Tsung-LinC & 葉錦瑩(Ching-YingY 2010, '地方性肢端壞疽症與麩胺基硫轉移酵素基因多型性關係之探討', 中南盟研究年刊, vol. 3, no. 1, pp. 1-16.
蔡光昭(Kuang-ChaoT, Chen R-Y, 許文憲(Wen-HaienH, 陳宗林(Tsung-LinC, 葉錦瑩(Ching-YingY. 地方性肢端壞疽症與麩胺基硫轉移酵素基因多型性關係之探討. 中南盟研究年刊. 2010 Mar 1;3(1):1-16.
蔡光昭(Kuang-Chao Tsai) ; Chen, Ruey-Yu ; 許文憲(Wen-Haien Hsu) ; 陳宗林(Tsung-Lin Chen) ; 葉錦瑩(Ching-Ying Yeh). / 地方性肢端壞疽症與麩胺基硫轉移酵素基因多型性關係之探討. In: 中南盟研究年刊. 2010 ; Vol. 3, No. 1. pp. 1-16.
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title = "地方性肢端壞疽症與麩胺基硫轉移酵素基因多型性關係之探討",
abstract = ":探討地方性肢端壞瘟症除神外之其他危險因子,並瞭解畫畫胺基硫轉移酵素基因多 型性對危險因子與疾病關係之影響。方法:於署立新營醫院完成的位病例組及48位對照組之 收案,每位參與者均收集調查問卷、血液及尿液檢體,並測定其生化值、多種金屬含量及畫畫 胺基硫轉移酵素基因多型性。結果:病例組之年齡、曾飲用井水、飲用井水年數、居住烏腳 病地區年數、吃舊書簽比率及尿中鉛含量均顯著高於對照組,而吃紅肉、內鵬、海鮮、香腸 火腿、水果、高纖或少鹽、煎或炸食物的比率,及血中盤胺基硫轉移酵素 CGSH) 、尿中錯含 量等則均顯著低於對照組;而遐胺基硫轉移酵素基因多型性鑑定結果發現, GST Pl基因型興 地方性服端壞瘟症的發生無顯著闇係,而病例組GST Ml GST T1皆為無效型之比例則顯著偏 CP<0.05) 。以羅吉斯複迴歸分析調整性別及年齡後顯示,居住烏腳病地區年數越久(每年 OR= 1. 0n GSH濃度越低(每 μM 之間=0.88)、 GST Ml GST T1皆為無效型者 COR=49.19) 較易 患地方性肢端壞瘟症,均達統計上顯著性,而常吃高纖或少鹽食物則稍有改善的作用 CO.05 <p<O .1)。結論:本研究並未能觀察到神的影響,但發現年齡越大、居住烏腳病地區年數越 久、 GSH濃度越低、 GST Ml GST T1皆為無效型者罹患地方性肢端壞瘟症的危險性較高",
keywords = "地方性肢端壞疽症, 烏腳病, 金屬元索, 基因多型性, endemic gangrenous panaritium, Black Foot Disease BFD, metallic elements , GST polymorphism",
author = "蔡, {光昭(Kuang-Chao Tsai)} and Ruey-Yu Chen and 許, {文憲(Wen-Haien Hsu)} and 陳, {宗林(Tsung-Lin Chen)} and 葉, {錦瑩(Ching-Ying Yeh)}",
year = "2010",
month = "3",
day = "1",
language = "繁體中文",
volume = "3",
pages = "1--16",
journal = "中南盟研究年刊",
issn = "2078-4856",
publisher = "行政院衛生署台中醫院",
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T1 - 地方性肢端壞疽症與麩胺基硫轉移酵素基因多型性關係之探討

AU - 蔡, 光昭(Kuang-Chao Tsai)

AU - Chen, Ruey-Yu

AU - 許, 文憲(Wen-Haien Hsu)

AU - 陳, 宗林(Tsung-Lin Chen)

AU - 葉, 錦瑩(Ching-Ying Yeh)

PY - 2010/3/1

Y1 - 2010/3/1

N2 - :探討地方性肢端壞瘟症除神外之其他危險因子,並瞭解畫畫胺基硫轉移酵素基因多 型性對危險因子與疾病關係之影響。方法:於署立新營醫院完成的位病例組及48位對照組之 收案,每位參與者均收集調查問卷、血液及尿液檢體,並測定其生化值、多種金屬含量及畫畫 胺基硫轉移酵素基因多型性。結果:病例組之年齡、曾飲用井水、飲用井水年數、居住烏腳 病地區年數、吃舊書簽比率及尿中鉛含量均顯著高於對照組,而吃紅肉、內鵬、海鮮、香腸 火腿、水果、高纖或少鹽、煎或炸食物的比率,及血中盤胺基硫轉移酵素 CGSH) 、尿中錯含 量等則均顯著低於對照組;而遐胺基硫轉移酵素基因多型性鑑定結果發現, GST Pl基因型興 地方性服端壞瘟症的發生無顯著闇係,而病例組GST Ml GST T1皆為無效型之比例則顯著偏 CP<0.05) 。以羅吉斯複迴歸分析調整性別及年齡後顯示,居住烏腳病地區年數越久(每年 OR= 1. 0n GSH濃度越低(每 μM 之間=0.88)、 GST Ml GST T1皆為無效型者 COR=49.19) 較易 患地方性肢端壞瘟症,均達統計上顯著性,而常吃高纖或少鹽食物則稍有改善的作用 CO.05 <p<O .1)。結論:本研究並未能觀察到神的影響,但發現年齡越大、居住烏腳病地區年數越 久、 GSH濃度越低、 GST Ml GST T1皆為無效型者罹患地方性肢端壞瘟症的危險性較高

AB - :探討地方性肢端壞瘟症除神外之其他危險因子,並瞭解畫畫胺基硫轉移酵素基因多 型性對危險因子與疾病關係之影響。方法:於署立新營醫院完成的位病例組及48位對照組之 收案,每位參與者均收集調查問卷、血液及尿液檢體,並測定其生化值、多種金屬含量及畫畫 胺基硫轉移酵素基因多型性。結果:病例組之年齡、曾飲用井水、飲用井水年數、居住烏腳 病地區年數、吃舊書簽比率及尿中鉛含量均顯著高於對照組,而吃紅肉、內鵬、海鮮、香腸 火腿、水果、高纖或少鹽、煎或炸食物的比率,及血中盤胺基硫轉移酵素 CGSH) 、尿中錯含 量等則均顯著低於對照組;而遐胺基硫轉移酵素基因多型性鑑定結果發現, GST Pl基因型興 地方性服端壞瘟症的發生無顯著闇係,而病例組GST Ml GST T1皆為無效型之比例則顯著偏 CP<0.05) 。以羅吉斯複迴歸分析調整性別及年齡後顯示,居住烏腳病地區年數越久(每年 OR= 1. 0n GSH濃度越低(每 μM 之間=0.88)、 GST Ml GST T1皆為無效型者 COR=49.19) 較易 患地方性肢端壞瘟症,均達統計上顯著性,而常吃高纖或少鹽食物則稍有改善的作用 CO.05 <p<O .1)。結論:本研究並未能觀察到神的影響,但發現年齡越大、居住烏腳病地區年數越 久、 GSH濃度越低、 GST Ml GST T1皆為無效型者罹患地方性肢端壞瘟症的危險性較高

KW - 地方性肢端壞疽症

KW - 烏腳病

KW - 金屬元索

KW - 基因多型性

KW - endemic gangrenous panaritium

KW - Black Foot Disease BFD

KW - metallic elements

KW - GST polymorphism

M3 - 文章

VL - 3

SP - 1

EP - 16

JO - 中南盟研究年刊

JF - 中南盟研究年刊

SN - 2078-4856

IS - 1

ER -