Assessment of authenticity of sesame oil by modified Villavecchia Test and HPLC-ELSD analysis of triacylglycerol profile

Wei Ju Lee, Nan Wei Su, Min Hsiung Lee, Jui Tsung Lin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Sesame oil is a nutritive and high-value edible oil. Concern about its adulteration has gained much attention. Here, we used a simple, modified colorimetric Villavecchia test and triacylglycerol (TAG) profile analysis to assess the authenticity of sesame oil. Sesame oil samples with 100-fold dilution and negative Villavecchia test results were blended sesame oil. Meanwhile, we developed a promising and feasible high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) analysis to compare the TAG profiles of sesame, soybean and canola oil. We found unique TAG species not existing in authentic sesame oil but occurring in soybean oil (dilinoleoyl linolenoyl glycerol (LLnL), oleoyl linolenoyl linoleoyl glycerol (OLnL) and palmitoyl linolenoyl linoleoyl glycerol (PLnL)) and canola oil (OLnL and dioleoyl linolenoyl glycerol (OLnO)), which could be used to judge the authenticity of sesame oil. Both first-order and quadratic regression curves for ELSD were feasible for determining the blending ratio of adulterated oil; however, the quadratic regression curve was recommended for quantification at a lower blending ratio because the first-order calibration curve would lead to underestimation of 0 to 20% adulteration and the corresponding intercept would be substantially negative.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalFood Research International
Volume53
Issue number1
DOIs
Publication statusPublished - Aug 1 2013
Externally publishedYes

Fingerprint

Sesame Oil
sesame oil
light scattering
detectors
Triglycerides
high performance liquid chromatography
triacylglycerols
High Pressure Liquid Chromatography
Light
glycerol
Glycerol
testing
Soybean Oil
canola oil
adulterated products
soybean oil
Oils
oils
Nutritive Value
Calibration

Keywords

  • Adulteration
  • Sesame oil
  • Triacylglycerol
  • Villavecchia test

ASJC Scopus subject areas

  • Food Science

Cite this

Assessment of authenticity of sesame oil by modified Villavecchia Test and HPLC-ELSD analysis of triacylglycerol profile. / Lee, Wei Ju; Su, Nan Wei; Lee, Min Hsiung; Lin, Jui Tsung.

In: Food Research International, Vol. 53, No. 1, 01.08.2013, p. 195-202.

Research output: Contribution to journalArticle

@article{4bbfa6f348f14793b75245c06e1cfd1a,
title = "Assessment of authenticity of sesame oil by modified Villavecchia Test and HPLC-ELSD analysis of triacylglycerol profile",
abstract = "Sesame oil is a nutritive and high-value edible oil. Concern about its adulteration has gained much attention. Here, we used a simple, modified colorimetric Villavecchia test and triacylglycerol (TAG) profile analysis to assess the authenticity of sesame oil. Sesame oil samples with 100-fold dilution and negative Villavecchia test results were blended sesame oil. Meanwhile, we developed a promising and feasible high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) analysis to compare the TAG profiles of sesame, soybean and canola oil. We found unique TAG species not existing in authentic sesame oil but occurring in soybean oil (dilinoleoyl linolenoyl glycerol (LLnL), oleoyl linolenoyl linoleoyl glycerol (OLnL) and palmitoyl linolenoyl linoleoyl glycerol (PLnL)) and canola oil (OLnL and dioleoyl linolenoyl glycerol (OLnO)), which could be used to judge the authenticity of sesame oil. Both first-order and quadratic regression curves for ELSD were feasible for determining the blending ratio of adulterated oil; however, the quadratic regression curve was recommended for quantification at a lower blending ratio because the first-order calibration curve would lead to underestimation of 0 to 20{\%} adulteration and the corresponding intercept would be substantially negative.",
keywords = "Adulteration, Sesame oil, Triacylglycerol, Villavecchia test",
author = "Lee, {Wei Ju} and Su, {Nan Wei} and Lee, {Min Hsiung} and Lin, {Jui Tsung}",
year = "2013",
month = "8",
day = "1",
doi = "10.1016/j.foodres.2013.04.008",
language = "English",
volume = "53",
pages = "195--202",
journal = "Food Research International",
issn = "0963-9969",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Assessment of authenticity of sesame oil by modified Villavecchia Test and HPLC-ELSD analysis of triacylglycerol profile

AU - Lee, Wei Ju

AU - Su, Nan Wei

AU - Lee, Min Hsiung

AU - Lin, Jui Tsung

PY - 2013/8/1

Y1 - 2013/8/1

N2 - Sesame oil is a nutritive and high-value edible oil. Concern about its adulteration has gained much attention. Here, we used a simple, modified colorimetric Villavecchia test and triacylglycerol (TAG) profile analysis to assess the authenticity of sesame oil. Sesame oil samples with 100-fold dilution and negative Villavecchia test results were blended sesame oil. Meanwhile, we developed a promising and feasible high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) analysis to compare the TAG profiles of sesame, soybean and canola oil. We found unique TAG species not existing in authentic sesame oil but occurring in soybean oil (dilinoleoyl linolenoyl glycerol (LLnL), oleoyl linolenoyl linoleoyl glycerol (OLnL) and palmitoyl linolenoyl linoleoyl glycerol (PLnL)) and canola oil (OLnL and dioleoyl linolenoyl glycerol (OLnO)), which could be used to judge the authenticity of sesame oil. Both first-order and quadratic regression curves for ELSD were feasible for determining the blending ratio of adulterated oil; however, the quadratic regression curve was recommended for quantification at a lower blending ratio because the first-order calibration curve would lead to underestimation of 0 to 20% adulteration and the corresponding intercept would be substantially negative.

AB - Sesame oil is a nutritive and high-value edible oil. Concern about its adulteration has gained much attention. Here, we used a simple, modified colorimetric Villavecchia test and triacylglycerol (TAG) profile analysis to assess the authenticity of sesame oil. Sesame oil samples with 100-fold dilution and negative Villavecchia test results were blended sesame oil. Meanwhile, we developed a promising and feasible high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) analysis to compare the TAG profiles of sesame, soybean and canola oil. We found unique TAG species not existing in authentic sesame oil but occurring in soybean oil (dilinoleoyl linolenoyl glycerol (LLnL), oleoyl linolenoyl linoleoyl glycerol (OLnL) and palmitoyl linolenoyl linoleoyl glycerol (PLnL)) and canola oil (OLnL and dioleoyl linolenoyl glycerol (OLnO)), which could be used to judge the authenticity of sesame oil. Both first-order and quadratic regression curves for ELSD were feasible for determining the blending ratio of adulterated oil; however, the quadratic regression curve was recommended for quantification at a lower blending ratio because the first-order calibration curve would lead to underestimation of 0 to 20% adulteration and the corresponding intercept would be substantially negative.

KW - Adulteration

KW - Sesame oil

KW - Triacylglycerol

KW - Villavecchia test

UR - http://www.scopus.com/inward/record.url?scp=84878407317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878407317&partnerID=8YFLogxK

U2 - 10.1016/j.foodres.2013.04.008

DO - 10.1016/j.foodres.2013.04.008

M3 - Article

VL - 53

SP - 195

EP - 202

JO - Food Research International

JF - Food Research International

SN - 0963-9969

IS - 1

ER -