Abstract
Atherosclerosis and its complications such as stroke, myocardial infraction and peripheral vascular disease, remain the major causes of morbidity and mortality in the world. Studies have showed that chemokines and adhesion molecules are involved in causing atherosclerosis by promoting directed migration of inflammatory cells. Monocyte chemoattractant protein-1 (MCP-1) is one of the key factors critical for the initiating and developing of atherosclerotic lesions. IL-8, a CXC chemokine, stimulates neutrophil chemotaxis. Aspirin is the most common drug used to prevent the complications of atherosclerosis such as stroke and coronary heart disease. In this study, we found that aspirin inhibited TNF-α (10 ng/ml)-induced MCP-1 and IL-8 expression at the RNA and protein levels in human umbilical vein endothelial cells (HUVECs), monocyte adhesion and transmigration, and that its inhibitory effects were not due to decreased HUVEC viability as assessed by MTT test. Aspirin at the dose as low as 10 μg/ml significantly inhibited the release of TNF-stimulated MCP-1 by 29.1% (P=0.008) and IL-8 by 26.9% (P=0.0146) as compared to TNF-stimulated release. Antibodies pretreatment were likely to decrease the production of MCP-1 (P
| Original language | English |
|---|---|
| Pages (from-to) | 207-213 |
| Number of pages | 7 |
| Journal | Atherosclerosis |
| Volume | 174 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 2004 |
| Externally published | Yes |
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Keywords
- Aspirin
- Atherosclerosis
- human umbilical vein endothelial cells
- Human vascular endothelial cell
- HUVEC
- IL-8
- interleukin-8
- MCP-1
- Monocyte chemoattractant protein-1
- monocyte chemoattractant protein-1
- TNF
- tumor necrosis factor
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Aspirin inhibits monocyte chemoattractant protein-1 and interleukin-8 expression in TNF-α stimulated human umbilical vein endothelial cells. / Yang, Yi Yuan; Hu, Chaur Jong; Chang, Su Mei; Tai, Tzu Yi; Leu, Sy Jye.
In: Atherosclerosis, Vol. 174, No. 2, 06.2004, p. 207-213.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aspirin inhibits monocyte chemoattractant protein-1 and interleukin-8 expression in TNF-α stimulated human umbilical vein endothelial cells
AU - Yang, Yi Yuan
AU - Hu, Chaur Jong
AU - Chang, Su Mei
AU - Tai, Tzu Yi
AU - Leu, Sy Jye
PY - 2004/6
Y1 - 2004/6
N2 - Atherosclerosis and its complications such as stroke, myocardial infraction and peripheral vascular disease, remain the major causes of morbidity and mortality in the world. Studies have showed that chemokines and adhesion molecules are involved in causing atherosclerosis by promoting directed migration of inflammatory cells. Monocyte chemoattractant protein-1 (MCP-1) is one of the key factors critical for the initiating and developing of atherosclerotic lesions. IL-8, a CXC chemokine, stimulates neutrophil chemotaxis. Aspirin is the most common drug used to prevent the complications of atherosclerosis such as stroke and coronary heart disease. In this study, we found that aspirin inhibited TNF-α (10 ng/ml)-induced MCP-1 and IL-8 expression at the RNA and protein levels in human umbilical vein endothelial cells (HUVECs), monocyte adhesion and transmigration, and that its inhibitory effects were not due to decreased HUVEC viability as assessed by MTT test. Aspirin at the dose as low as 10 μg/ml significantly inhibited the release of TNF-stimulated MCP-1 by 29.1% (P=0.008) and IL-8 by 26.9% (P=0.0146) as compared to TNF-stimulated release. Antibodies pretreatment were likely to decrease the production of MCP-1 (P
AB - Atherosclerosis and its complications such as stroke, myocardial infraction and peripheral vascular disease, remain the major causes of morbidity and mortality in the world. Studies have showed that chemokines and adhesion molecules are involved in causing atherosclerosis by promoting directed migration of inflammatory cells. Monocyte chemoattractant protein-1 (MCP-1) is one of the key factors critical for the initiating and developing of atherosclerotic lesions. IL-8, a CXC chemokine, stimulates neutrophil chemotaxis. Aspirin is the most common drug used to prevent the complications of atherosclerosis such as stroke and coronary heart disease. In this study, we found that aspirin inhibited TNF-α (10 ng/ml)-induced MCP-1 and IL-8 expression at the RNA and protein levels in human umbilical vein endothelial cells (HUVECs), monocyte adhesion and transmigration, and that its inhibitory effects were not due to decreased HUVEC viability as assessed by MTT test. Aspirin at the dose as low as 10 μg/ml significantly inhibited the release of TNF-stimulated MCP-1 by 29.1% (P=0.008) and IL-8 by 26.9% (P=0.0146) as compared to TNF-stimulated release. Antibodies pretreatment were likely to decrease the production of MCP-1 (P
KW - Aspirin
KW - Atherosclerosis
KW - human umbilical vein endothelial cells
KW - Human vascular endothelial cell
KW - HUVEC
KW - IL-8
KW - interleukin-8
KW - MCP-1
KW - Monocyte chemoattractant protein-1
KW - monocyte chemoattractant protein-1
KW - TNF
KW - tumor necrosis factor
UR - http://www.scopus.com/inward/record.url?scp=2342521248&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2342521248&partnerID=8YFLogxK
U2 - 10.1016/j.atherosclerosis.2004.01.024
DO - 10.1016/j.atherosclerosis.2004.01.024
M3 - Article
C2 - 15136050
AN - SCOPUS:2342521248
VL - 174
SP - 207
EP - 213
JO - Atherosclerosis
JF - Atherosclerosis
SN - 0021-9150
IS - 2
ER -