Arrayed rGOSH/PMASH microcapsule platform integrating surface topography, chemical cues, and electrical stimulation for three-dimensional neuron-like cell growth and neurite sprouting

Heng Wen Liu, Wei Chen Huang, Chih Sheng Chiang, Shang Hsiu Hu, Chia Hsin Liao, You Yin Chen, San Yuan Chen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The biocompatible thiol-functionalized rGOSH/PMASH microcapsules encapsulating nerve growth factor (NGF) are arrayed onto a transparent and conductive substrate, i.e., indium tin oxide (ITO), to integrate electrically stimulated cellular differentiation, electrically controlled NGF release, and topographically rough nano-surfaces into a 3-D platform for nerve regeneration. The rGOSH/PMASH microcapsules with microscale topography function not only as an adhesive coating to promote the adhesion of PC12 cells but also as electroactive NGF-releasing electrodes that stimulate NGF release and accelerate the differentiation of PC12 cells during electrical stimulation. Once electrical treatment is applied, NGF release and electrically enhanced cellular differentiation lead to an obvious increase both in the percentage of cells with neurites and in the neurite length. This length can reach nearly 90 μm within 2 days of cell culture. The average neurite length is significantly increased (four-fold) after culture on the rGO SH/PMASH microcapsule substrate for 2 days compared with culture on a substrate without an rGOSH/PMASH coating. These multifunctional rGOSH/PMASH microcapsules may be used as potential 3-D patterned substrates for neural regeneration and neural prosthetics in tissue engineering applications. The stimulus-responsive, well-ordered rGOSH/PMASH microcapsules are arrayed into a 3-D ECM-mimic flexible substrate to accelerate the proliferation and differentiation of PC12 cells by controlling NGF release and manipulating rGOSH/PMASH microcapsule interfaces. A combination of surface topography, chemical cues, and electrical stimulation not only has positive effects on cell viability but also strongly enhances the neurite outgrowth of PC12 cells.

Original languageEnglish
Pages (from-to)3715-3724
Number of pages10
JournalAdvanced Functional Materials
Volume24
Issue number24
DOIs
Publication statusPublished - Jun 25 2014
Externally publishedYes

Fingerprint

cues
nerves
Cell growth
Surface topography
Nerve Growth Factor
neurons
stimulation
Neurons
Capsules
topography
platforms
Substrates
cells
regeneration
Military electronic countermeasures
Coatings
coatings
encapsulating
Prosthetics
Tin oxides

Keywords

  • differentiation
  • graphene
  • microcapsules
  • neural cells
  • template

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Arrayed rGOSH/PMASH microcapsule platform integrating surface topography, chemical cues, and electrical stimulation for three-dimensional neuron-like cell growth and neurite sprouting. / Liu, Heng Wen; Huang, Wei Chen; Chiang, Chih Sheng; Hu, Shang Hsiu; Liao, Chia Hsin; Chen, You Yin; Chen, San Yuan.

In: Advanced Functional Materials, Vol. 24, No. 24, 25.06.2014, p. 3715-3724.

Research output: Contribution to journalArticle

Liu, Heng Wen ; Huang, Wei Chen ; Chiang, Chih Sheng ; Hu, Shang Hsiu ; Liao, Chia Hsin ; Chen, You Yin ; Chen, San Yuan. / Arrayed rGOSH/PMASH microcapsule platform integrating surface topography, chemical cues, and electrical stimulation for three-dimensional neuron-like cell growth and neurite sprouting. In: Advanced Functional Materials. 2014 ; Vol. 24, No. 24. pp. 3715-3724.
@article{ca8f7de29e7d4aebb2adeeca254e79c3,
title = "Arrayed rGOSH/PMASH microcapsule platform integrating surface topography, chemical cues, and electrical stimulation for three-dimensional neuron-like cell growth and neurite sprouting",
abstract = "The biocompatible thiol-functionalized rGOSH/PMASH microcapsules encapsulating nerve growth factor (NGF) are arrayed onto a transparent and conductive substrate, i.e., indium tin oxide (ITO), to integrate electrically stimulated cellular differentiation, electrically controlled NGF release, and topographically rough nano-surfaces into a 3-D platform for nerve regeneration. The rGOSH/PMASH microcapsules with microscale topography function not only as an adhesive coating to promote the adhesion of PC12 cells but also as electroactive NGF-releasing electrodes that stimulate NGF release and accelerate the differentiation of PC12 cells during electrical stimulation. Once electrical treatment is applied, NGF release and electrically enhanced cellular differentiation lead to an obvious increase both in the percentage of cells with neurites and in the neurite length. This length can reach nearly 90 μm within 2 days of cell culture. The average neurite length is significantly increased (four-fold) after culture on the rGO SH/PMASH microcapsule substrate for 2 days compared with culture on a substrate without an rGOSH/PMASH coating. These multifunctional rGOSH/PMASH microcapsules may be used as potential 3-D patterned substrates for neural regeneration and neural prosthetics in tissue engineering applications. The stimulus-responsive, well-ordered rGOSH/PMASH microcapsules are arrayed into a 3-D ECM-mimic flexible substrate to accelerate the proliferation and differentiation of PC12 cells by controlling NGF release and manipulating rGOSH/PMASH microcapsule interfaces. A combination of surface topography, chemical cues, and electrical stimulation not only has positive effects on cell viability but also strongly enhances the neurite outgrowth of PC12 cells.",
keywords = "differentiation, graphene, microcapsules, neural cells, template",
author = "Liu, {Heng Wen} and Huang, {Wei Chen} and Chiang, {Chih Sheng} and Hu, {Shang Hsiu} and Liao, {Chia Hsin} and Chen, {You Yin} and Chen, {San Yuan}",
year = "2014",
month = "6",
day = "25",
doi = "10.1002/adfm.201303853",
language = "English",
volume = "24",
pages = "3715--3724",
journal = "Advanced Materials for Optics and Electronics",
issn = "1057-9257",
publisher = "Wiley-Blackwell",
number = "24",

}

TY - JOUR

T1 - Arrayed rGOSH/PMASH microcapsule platform integrating surface topography, chemical cues, and electrical stimulation for three-dimensional neuron-like cell growth and neurite sprouting

AU - Liu, Heng Wen

AU - Huang, Wei Chen

AU - Chiang, Chih Sheng

AU - Hu, Shang Hsiu

AU - Liao, Chia Hsin

AU - Chen, You Yin

AU - Chen, San Yuan

PY - 2014/6/25

Y1 - 2014/6/25

N2 - The biocompatible thiol-functionalized rGOSH/PMASH microcapsules encapsulating nerve growth factor (NGF) are arrayed onto a transparent and conductive substrate, i.e., indium tin oxide (ITO), to integrate electrically stimulated cellular differentiation, electrically controlled NGF release, and topographically rough nano-surfaces into a 3-D platform for nerve regeneration. The rGOSH/PMASH microcapsules with microscale topography function not only as an adhesive coating to promote the adhesion of PC12 cells but also as electroactive NGF-releasing electrodes that stimulate NGF release and accelerate the differentiation of PC12 cells during electrical stimulation. Once electrical treatment is applied, NGF release and electrically enhanced cellular differentiation lead to an obvious increase both in the percentage of cells with neurites and in the neurite length. This length can reach nearly 90 μm within 2 days of cell culture. The average neurite length is significantly increased (four-fold) after culture on the rGO SH/PMASH microcapsule substrate for 2 days compared with culture on a substrate without an rGOSH/PMASH coating. These multifunctional rGOSH/PMASH microcapsules may be used as potential 3-D patterned substrates for neural regeneration and neural prosthetics in tissue engineering applications. The stimulus-responsive, well-ordered rGOSH/PMASH microcapsules are arrayed into a 3-D ECM-mimic flexible substrate to accelerate the proliferation and differentiation of PC12 cells by controlling NGF release and manipulating rGOSH/PMASH microcapsule interfaces. A combination of surface topography, chemical cues, and electrical stimulation not only has positive effects on cell viability but also strongly enhances the neurite outgrowth of PC12 cells.

AB - The biocompatible thiol-functionalized rGOSH/PMASH microcapsules encapsulating nerve growth factor (NGF) are arrayed onto a transparent and conductive substrate, i.e., indium tin oxide (ITO), to integrate electrically stimulated cellular differentiation, electrically controlled NGF release, and topographically rough nano-surfaces into a 3-D platform for nerve regeneration. The rGOSH/PMASH microcapsules with microscale topography function not only as an adhesive coating to promote the adhesion of PC12 cells but also as electroactive NGF-releasing electrodes that stimulate NGF release and accelerate the differentiation of PC12 cells during electrical stimulation. Once electrical treatment is applied, NGF release and electrically enhanced cellular differentiation lead to an obvious increase both in the percentage of cells with neurites and in the neurite length. This length can reach nearly 90 μm within 2 days of cell culture. The average neurite length is significantly increased (four-fold) after culture on the rGO SH/PMASH microcapsule substrate for 2 days compared with culture on a substrate without an rGOSH/PMASH coating. These multifunctional rGOSH/PMASH microcapsules may be used as potential 3-D patterned substrates for neural regeneration and neural prosthetics in tissue engineering applications. The stimulus-responsive, well-ordered rGOSH/PMASH microcapsules are arrayed into a 3-D ECM-mimic flexible substrate to accelerate the proliferation and differentiation of PC12 cells by controlling NGF release and manipulating rGOSH/PMASH microcapsule interfaces. A combination of surface topography, chemical cues, and electrical stimulation not only has positive effects on cell viability but also strongly enhances the neurite outgrowth of PC12 cells.

KW - differentiation

KW - graphene

KW - microcapsules

KW - neural cells

KW - template

UR - http://www.scopus.com/inward/record.url?scp=84903203357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903203357&partnerID=8YFLogxK

U2 - 10.1002/adfm.201303853

DO - 10.1002/adfm.201303853

M3 - Article

AN - SCOPUS:84903203357

VL - 24

SP - 3715

EP - 3724

JO - Advanced Materials for Optics and Electronics

JF - Advanced Materials for Optics and Electronics

SN - 1057-9257

IS - 24

ER -