Armillaria mellea component armillarikin induces apoptosis in human leukemia cells

Yu Jen Chen, Szu-Yuan Wu, Chien Chih Chen, Yu Lin Tsao, Nai Chi Hsu, Yu Chi Chou, Huey Lan Huang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Honey mushroom Armillaria mellea, a commonly used medicinal food in Asia, is an important component of traditional Chinese medicine "Tien-ma". Armillarikin is a compound isolated from A. mellea with unclear biomedical functions. In this study, we found that armillarikin inhibited the viability of human leukemia K562, U937, and HL-60 cells in a concentration-dependent manner, causing cell death mainly attributable to apoptosis accompanied by reduction of mitochondrial transmembrane potential. Armillarikin induced cleavage of caspase-8, caspase-9, caspase-3, and caspase-3 substrate PARP. Armillarikin-induced growth inhibition and apoptosis were reversed by pan-caspase inhibitor Z-VAD-fmk, indicating a caspase-dependent effect. Moreover, armillarikin increased production of intracellular ROS. Prevention of ROS production attenuated armillarikin-induced apoptosis, suggesting mediation by ROS. In conclusion, armillarikin inhibited growth and induced apoptosis through a mechanism involving mitochondria dysfunction, caspase activation and ROS production in human leukemia K562, U937, and HL-60 cells.

Original languageEnglish
Pages (from-to)196-204
Number of pages9
JournalJournal of Functional Foods
Volume6
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Armillaria
Armillaria mellea
leukemia
caspases
Leukemia
apoptosis
Apoptosis
HL-60 Cells
caspase-3
Caspases
Caspase 3
Gastrodia
cells
caspase-8
caspase-9
Caspase Inhibitors
Honey
Caspase 9
Caspase 8
Agaricales

Keywords

  • Apoptosis
  • Armillaria mellea
  • Armillarikin
  • Leukemia
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Armillaria mellea component armillarikin induces apoptosis in human leukemia cells. / Chen, Yu Jen; Wu, Szu-Yuan; Chen, Chien Chih; Tsao, Yu Lin; Hsu, Nai Chi; Chou, Yu Chi; Huang, Huey Lan.

In: Journal of Functional Foods, Vol. 6, No. 1, 2014, p. 196-204.

Research output: Contribution to journalArticle

Chen, Yu Jen ; Wu, Szu-Yuan ; Chen, Chien Chih ; Tsao, Yu Lin ; Hsu, Nai Chi ; Chou, Yu Chi ; Huang, Huey Lan. / Armillaria mellea component armillarikin induces apoptosis in human leukemia cells. In: Journal of Functional Foods. 2014 ; Vol. 6, No. 1. pp. 196-204.
@article{bf44ad30ac464621be82deb80ce873c1,
title = "Armillaria mellea component armillarikin induces apoptosis in human leukemia cells",
abstract = "Honey mushroom Armillaria mellea, a commonly used medicinal food in Asia, is an important component of traditional Chinese medicine {"}Tien-ma{"}. Armillarikin is a compound isolated from A. mellea with unclear biomedical functions. In this study, we found that armillarikin inhibited the viability of human leukemia K562, U937, and HL-60 cells in a concentration-dependent manner, causing cell death mainly attributable to apoptosis accompanied by reduction of mitochondrial transmembrane potential. Armillarikin induced cleavage of caspase-8, caspase-9, caspase-3, and caspase-3 substrate PARP. Armillarikin-induced growth inhibition and apoptosis were reversed by pan-caspase inhibitor Z-VAD-fmk, indicating a caspase-dependent effect. Moreover, armillarikin increased production of intracellular ROS. Prevention of ROS production attenuated armillarikin-induced apoptosis, suggesting mediation by ROS. In conclusion, armillarikin inhibited growth and induced apoptosis through a mechanism involving mitochondria dysfunction, caspase activation and ROS production in human leukemia K562, U937, and HL-60 cells.",
keywords = "Apoptosis, Armillaria mellea, Armillarikin, Leukemia, Reactive oxygen species (ROS)",
author = "Chen, {Yu Jen} and Szu-Yuan Wu and Chen, {Chien Chih} and Tsao, {Yu Lin} and Hsu, {Nai Chi} and Chou, {Yu Chi} and Huang, {Huey Lan}",
year = "2014",
doi = "10.1016/j.jff.2013.10.007",
language = "English",
volume = "6",
pages = "196--204",
journal = "Journal of Functional Foods",
issn = "1756-4646",
publisher = "Elsevier Ltd",
number = "1",

}

TY - JOUR

T1 - Armillaria mellea component armillarikin induces apoptosis in human leukemia cells

AU - Chen, Yu Jen

AU - Wu, Szu-Yuan

AU - Chen, Chien Chih

AU - Tsao, Yu Lin

AU - Hsu, Nai Chi

AU - Chou, Yu Chi

AU - Huang, Huey Lan

PY - 2014

Y1 - 2014

N2 - Honey mushroom Armillaria mellea, a commonly used medicinal food in Asia, is an important component of traditional Chinese medicine "Tien-ma". Armillarikin is a compound isolated from A. mellea with unclear biomedical functions. In this study, we found that armillarikin inhibited the viability of human leukemia K562, U937, and HL-60 cells in a concentration-dependent manner, causing cell death mainly attributable to apoptosis accompanied by reduction of mitochondrial transmembrane potential. Armillarikin induced cleavage of caspase-8, caspase-9, caspase-3, and caspase-3 substrate PARP. Armillarikin-induced growth inhibition and apoptosis were reversed by pan-caspase inhibitor Z-VAD-fmk, indicating a caspase-dependent effect. Moreover, armillarikin increased production of intracellular ROS. Prevention of ROS production attenuated armillarikin-induced apoptosis, suggesting mediation by ROS. In conclusion, armillarikin inhibited growth and induced apoptosis through a mechanism involving mitochondria dysfunction, caspase activation and ROS production in human leukemia K562, U937, and HL-60 cells.

AB - Honey mushroom Armillaria mellea, a commonly used medicinal food in Asia, is an important component of traditional Chinese medicine "Tien-ma". Armillarikin is a compound isolated from A. mellea with unclear biomedical functions. In this study, we found that armillarikin inhibited the viability of human leukemia K562, U937, and HL-60 cells in a concentration-dependent manner, causing cell death mainly attributable to apoptosis accompanied by reduction of mitochondrial transmembrane potential. Armillarikin induced cleavage of caspase-8, caspase-9, caspase-3, and caspase-3 substrate PARP. Armillarikin-induced growth inhibition and apoptosis were reversed by pan-caspase inhibitor Z-VAD-fmk, indicating a caspase-dependent effect. Moreover, armillarikin increased production of intracellular ROS. Prevention of ROS production attenuated armillarikin-induced apoptosis, suggesting mediation by ROS. In conclusion, armillarikin inhibited growth and induced apoptosis through a mechanism involving mitochondria dysfunction, caspase activation and ROS production in human leukemia K562, U937, and HL-60 cells.

KW - Apoptosis

KW - Armillaria mellea

KW - Armillarikin

KW - Leukemia

KW - Reactive oxygen species (ROS)

UR - http://www.scopus.com/inward/record.url?scp=84895071518&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84895071518&partnerID=8YFLogxK

U2 - 10.1016/j.jff.2013.10.007

DO - 10.1016/j.jff.2013.10.007

M3 - Article

AN - SCOPUS:84895071518

VL - 6

SP - 196

EP - 204

JO - Journal of Functional Foods

JF - Journal of Functional Foods

SN - 1756-4646

IS - 1

ER -