Areca quid chewing enhances the expression of salivary matrix metalloproteinase-9

Shyun Y. Hu, Mei Huei Lin, Shun Chun Yang, Guan Cheng Huang, Lisa Chang, Sam Chang, Ching Y. Yen, Wei F. Chiang, Chin H. Lee, Yung Y. Kuo, Young Chau Liu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background and Purpose: The effects of areca quid (AQ) consumption on salivary matrix metalloproteinases (MMPs) which may participate in tumor invasion and metastasis remains unclear. This study assessed the change in salivary MMP-9 protein levels 2 hours after 5-minute AQ chewing stimulation (AQCS) in non-AQ users and the expression profile of this proteinase in saliva and tumor specimens of oral squamous cell carcinoma (OSCC) patients with a history of AQ use. Methods: MMP-9 transcript was analyzed by reverse transcription-polymerase chain reaction. MMP-9 protein level was measured by both Western blot and gelatin zymography. Results: The protein level of salivary MMP-9 was 3.1- to 8.9-fold enhanced 2 h after AQCS in 3 healthy volunteers as revealed by Western blot and zymography. As a control, gum chewing did not significantly change salivary MMP-9 protein level. Expression of MMP-9 transcript was found in 25 of 28 OSCC specimens and significantly correlated with cervical lymph node metastasis (p = 0.037). All of the 8 tested OSCC tissue homogenate samples available and all 12 saliva samples from 12 oral tumor outpatients were positive for MMP-9 protein. Conclusions: Elevation of MMP-9 may be one of the net effects of AQCS in vivo, which may play a role in the pathogenesis of oral mucosal lesions. Furthermore, the association of MMP-9 expression with neck-lymph-node metastasis may imply a significant role of MMP-9 in the progression of OSCC among patients with a history of AQ use in Taiwan.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume104
Issue number2
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Areca
Mastication
Matrix Metalloproteinase 9
Squamous Cell Carcinoma
Neoplasm Metastasis
Saliva
Proteins
Lymph Nodes
Western Blotting
Chewing Gum
Salivary Proteins and Peptides
Neoplasms
Gelatin
Matrix Metalloproteinases
Taiwan
Reverse Transcription
Healthy Volunteers
Peptide Hydrolases
Outpatients
Neck

Keywords

  • Arecoline
  • Gelatinase B
  • Matrix metalloproteinases
  • Saliva

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hu, S. Y., Lin, M. H., Yang, S. C., Huang, G. C., Chang, L., Chang, S., ... Liu, Y. C. (2005). Areca quid chewing enhances the expression of salivary matrix metalloproteinase-9. Journal of the Formosan Medical Association = Taiwan yi zhi, 104(2), 113-119.

Areca quid chewing enhances the expression of salivary matrix metalloproteinase-9. / Hu, Shyun Y.; Lin, Mei Huei; Yang, Shun Chun; Huang, Guan Cheng; Chang, Lisa; Chang, Sam; Yen, Ching Y.; Chiang, Wei F.; Lee, Chin H.; Kuo, Yung Y.; Liu, Young Chau.

In: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 104, No. 2, 2005, p. 113-119.

Research output: Contribution to journalArticle

Hu, SY, Lin, MH, Yang, SC, Huang, GC, Chang, L, Chang, S, Yen, CY, Chiang, WF, Lee, CH, Kuo, YY & Liu, YC 2005, 'Areca quid chewing enhances the expression of salivary matrix metalloproteinase-9', Journal of the Formosan Medical Association = Taiwan yi zhi, vol. 104, no. 2, pp. 113-119.
Hu, Shyun Y. ; Lin, Mei Huei ; Yang, Shun Chun ; Huang, Guan Cheng ; Chang, Lisa ; Chang, Sam ; Yen, Ching Y. ; Chiang, Wei F. ; Lee, Chin H. ; Kuo, Yung Y. ; Liu, Young Chau. / Areca quid chewing enhances the expression of salivary matrix metalloproteinase-9. In: Journal of the Formosan Medical Association = Taiwan yi zhi. 2005 ; Vol. 104, No. 2. pp. 113-119.
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abstract = "Background and Purpose: The effects of areca quid (AQ) consumption on salivary matrix metalloproteinases (MMPs) which may participate in tumor invasion and metastasis remains unclear. This study assessed the change in salivary MMP-9 protein levels 2 hours after 5-minute AQ chewing stimulation (AQCS) in non-AQ users and the expression profile of this proteinase in saliva and tumor specimens of oral squamous cell carcinoma (OSCC) patients with a history of AQ use. Methods: MMP-9 transcript was analyzed by reverse transcription-polymerase chain reaction. MMP-9 protein level was measured by both Western blot and gelatin zymography. Results: The protein level of salivary MMP-9 was 3.1- to 8.9-fold enhanced 2 h after AQCS in 3 healthy volunteers as revealed by Western blot and zymography. As a control, gum chewing did not significantly change salivary MMP-9 protein level. Expression of MMP-9 transcript was found in 25 of 28 OSCC specimens and significantly correlated with cervical lymph node metastasis (p = 0.037). All of the 8 tested OSCC tissue homogenate samples available and all 12 saliva samples from 12 oral tumor outpatients were positive for MMP-9 protein. Conclusions: Elevation of MMP-9 may be one of the net effects of AQCS in vivo, which may play a role in the pathogenesis of oral mucosal lesions. Furthermore, the association of MMP-9 expression with neck-lymph-node metastasis may imply a significant role of MMP-9 in the progression of OSCC among patients with a history of AQ use in Taiwan.",
keywords = "Arecoline, Gelatinase B, Matrix metalloproteinases, Saliva",
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AU - Hu, Shyun Y.

AU - Lin, Mei Huei

AU - Yang, Shun Chun

AU - Huang, Guan Cheng

AU - Chang, Lisa

AU - Chang, Sam

AU - Yen, Ching Y.

AU - Chiang, Wei F.

AU - Lee, Chin H.

AU - Kuo, Yung Y.

AU - Liu, Young Chau

PY - 2005

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N2 - Background and Purpose: The effects of areca quid (AQ) consumption on salivary matrix metalloproteinases (MMPs) which may participate in tumor invasion and metastasis remains unclear. This study assessed the change in salivary MMP-9 protein levels 2 hours after 5-minute AQ chewing stimulation (AQCS) in non-AQ users and the expression profile of this proteinase in saliva and tumor specimens of oral squamous cell carcinoma (OSCC) patients with a history of AQ use. Methods: MMP-9 transcript was analyzed by reverse transcription-polymerase chain reaction. MMP-9 protein level was measured by both Western blot and gelatin zymography. Results: The protein level of salivary MMP-9 was 3.1- to 8.9-fold enhanced 2 h after AQCS in 3 healthy volunteers as revealed by Western blot and zymography. As a control, gum chewing did not significantly change salivary MMP-9 protein level. Expression of MMP-9 transcript was found in 25 of 28 OSCC specimens and significantly correlated with cervical lymph node metastasis (p = 0.037). All of the 8 tested OSCC tissue homogenate samples available and all 12 saliva samples from 12 oral tumor outpatients were positive for MMP-9 protein. Conclusions: Elevation of MMP-9 may be one of the net effects of AQCS in vivo, which may play a role in the pathogenesis of oral mucosal lesions. Furthermore, the association of MMP-9 expression with neck-lymph-node metastasis may imply a significant role of MMP-9 in the progression of OSCC among patients with a history of AQ use in Taiwan.

AB - Background and Purpose: The effects of areca quid (AQ) consumption on salivary matrix metalloproteinases (MMPs) which may participate in tumor invasion and metastasis remains unclear. This study assessed the change in salivary MMP-9 protein levels 2 hours after 5-minute AQ chewing stimulation (AQCS) in non-AQ users and the expression profile of this proteinase in saliva and tumor specimens of oral squamous cell carcinoma (OSCC) patients with a history of AQ use. Methods: MMP-9 transcript was analyzed by reverse transcription-polymerase chain reaction. MMP-9 protein level was measured by both Western blot and gelatin zymography. Results: The protein level of salivary MMP-9 was 3.1- to 8.9-fold enhanced 2 h after AQCS in 3 healthy volunteers as revealed by Western blot and zymography. As a control, gum chewing did not significantly change salivary MMP-9 protein level. Expression of MMP-9 transcript was found in 25 of 28 OSCC specimens and significantly correlated with cervical lymph node metastasis (p = 0.037). All of the 8 tested OSCC tissue homogenate samples available and all 12 saliva samples from 12 oral tumor outpatients were positive for MMP-9 protein. Conclusions: Elevation of MMP-9 may be one of the net effects of AQCS in vivo, which may play a role in the pathogenesis of oral mucosal lesions. Furthermore, the association of MMP-9 expression with neck-lymph-node metastasis may imply a significant role of MMP-9 in the progression of OSCC among patients with a history of AQ use in Taiwan.

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