Areca nut extract treatment elicits the fibroblastoid morphological changes, actin re-organization and signaling activation in oral keratinocytes

Shun Chun Yang, Shu Chun Lin, Wei Fan Chiang, Ching Yu Yen, Chi Hung Lin, Shyun Yeu Liu

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background: Areca (named as betel) is an important etiological factor linked with the high prevalence of oral squamous cell carcinoma (OSCC) in South-Asian countries. This in vitro study investigated the cellular changes and signaling activation in oral keratinocytes in response to areca nut extract (ANE) treatment. Methods: Normal human oral keratinocyte (NHOK) and oral epidermoid carcinoma cell, Meng-1 (OECM-1) OSCC cell line were treated with variable dosages of ripen ANE. The morphological and cytoskeletal changes, as well as the activation of GTPase proteins and signaling kinases, were analyzed. Results: Most NHOK cells in culture were polygonal, with only <5% cells exhibiting fibroblastoid morphology. However, 10 μg/ml ANE elicited fibroblastoid morphological change, genesis of lamellipodia, loss of subcortical actin, and stress-fiber formation in approximately 25% cultivated NHOK cells. Similar morphological changes were observed in nearly all OECM-1 cells following the ANE treatment. The activation of Rac and Rho GTPase, together with the prominent phosphorylation of a stress-activated kinases, particularly JNK1, was identified in treated OECM-1 cells. Conclusion: The novel evidences from the study that ANE impairs the actin organization and activates the signals in oral keratinocytes might bestow further insight into the impacts of ANE in oral pathogenesis.

Original languageEnglish
Pages (from-to)600-605
Number of pages6
JournalJournal of Oral Pathology and Medicine
Volume32
Issue number10
DOIs
Publication statusPublished - Nov 2003
Externally publishedYes

Fingerprint

Areca
Nuts
Keratinocytes
Actins
Squamous Cell Carcinoma
Mitogen-Activated Protein Kinase 8
Stress Fibers
rho GTP-Binding Proteins
Pseudopodia
GTP Phosphohydrolases
Protein Kinases
Cell Culture Techniques
Phosphorylation
Cell Line

Keywords

  • Areca
  • GTPase
  • Mouth
  • Neoplasm

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Dentistry(all)

Cite this

Areca nut extract treatment elicits the fibroblastoid morphological changes, actin re-organization and signaling activation in oral keratinocytes. / Yang, Shun Chun; Lin, Shu Chun; Chiang, Wei Fan; Yen, Ching Yu; Lin, Chi Hung; Liu, Shyun Yeu.

In: Journal of Oral Pathology and Medicine, Vol. 32, No. 10, 11.2003, p. 600-605.

Research output: Contribution to journalArticle

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