Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes

Wei Jern Tsai, Chu Ting Chang, Guei Jane Wang, Zong-Huei Li, Shwu Fen Chang, Shao Chun Lu, Yuh Chi Kuo

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Arctium lappa (Niubang), a Chinese herbal medicine, is used to treat tissue inflammation. This study investigates the effects of arctigenin (AC), isolated from A. lappa, on anti-CD3/CD28 Ab-stimulated cell proliferation and cytokine gene expression in primary human T lymphocytes.Methods: Cell proliferation was determined with enzyme immunoassays and the tritiated thymidine uptake method. Cytokine production and gene expression were analyzed with reverse transcription-polymerase chain reaction.Results: AC inhibited primary human T lymphocytes proliferation activated by anti-CD3/CD28 Ab. Cell viability test indicated that the inhibitory effects of AC on primary human T lymphocyte proliferation were not due to direct cytotoxicity. AC suppressed interleukin-2 (IL-2) and interferon-γ (IFN-γ) production in a concentration-dependent manner. Furthermore, AC decreased the IL-2 and IFN-γ gene expression in primary human T lymphocytes induced by anti-CD3/CD28 Ab. Reporter gene analyses revealed that AC decreased NF-AT-mediated reporter gene expression.Conclusion: AC inhibited T lymphocyte proliferation and decreased the gene expression of IL-2, IFN-γ and NF-AT.

Original languageEnglish
Article number12
JournalChinese Medicine
Volume6
DOIs
Publication statusPublished - Mar 25 2011

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Arctium
Interferons
Interleukin-2
T-Lymphocytes
Gene Expression
Reporter Genes
Cell Proliferation
Cytokines
Herbal Medicine
arctigenin
Immunoenzyme Techniques
Thymidine
Reverse Transcription
Cell Survival
Inflammation
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Pharmacology

Cite this

Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes. / Tsai, Wei Jern; Chang, Chu Ting; Wang, Guei Jane; Li, Zong-Huei; Chang, Shwu Fen; Lu, Shao Chun; Kuo, Yuh Chi.

In: Chinese Medicine, Vol. 6, 12, 25.03.2011.

Research output: Contribution to journalArticle

Tsai, Wei Jern ; Chang, Chu Ting ; Wang, Guei Jane ; Li, Zong-Huei ; Chang, Shwu Fen ; Lu, Shao Chun ; Kuo, Yuh Chi. / Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes. In: Chinese Medicine. 2011 ; Vol. 6.
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abstract = "Background: Arctium lappa (Niubang), a Chinese herbal medicine, is used to treat tissue inflammation. This study investigates the effects of arctigenin (AC), isolated from A. lappa, on anti-CD3/CD28 Ab-stimulated cell proliferation and cytokine gene expression in primary human T lymphocytes.Methods: Cell proliferation was determined with enzyme immunoassays and the tritiated thymidine uptake method. Cytokine production and gene expression were analyzed with reverse transcription-polymerase chain reaction.Results: AC inhibited primary human T lymphocytes proliferation activated by anti-CD3/CD28 Ab. Cell viability test indicated that the inhibitory effects of AC on primary human T lymphocyte proliferation were not due to direct cytotoxicity. AC suppressed interleukin-2 (IL-2) and interferon-γ (IFN-γ) production in a concentration-dependent manner. Furthermore, AC decreased the IL-2 and IFN-γ gene expression in primary human T lymphocytes induced by anti-CD3/CD28 Ab. Reporter gene analyses revealed that AC decreased NF-AT-mediated reporter gene expression.Conclusion: AC inhibited T lymphocyte proliferation and decreased the gene expression of IL-2, IFN-γ and NF-AT.",
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AU - Tsai, Wei Jern

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AU - Wang, Guei Jane

AU - Li, Zong-Huei

AU - Chang, Shwu Fen

AU - Lu, Shao Chun

AU - Kuo, Yuh Chi

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N2 - Background: Arctium lappa (Niubang), a Chinese herbal medicine, is used to treat tissue inflammation. This study investigates the effects of arctigenin (AC), isolated from A. lappa, on anti-CD3/CD28 Ab-stimulated cell proliferation and cytokine gene expression in primary human T lymphocytes.Methods: Cell proliferation was determined with enzyme immunoassays and the tritiated thymidine uptake method. Cytokine production and gene expression were analyzed with reverse transcription-polymerase chain reaction.Results: AC inhibited primary human T lymphocytes proliferation activated by anti-CD3/CD28 Ab. Cell viability test indicated that the inhibitory effects of AC on primary human T lymphocyte proliferation were not due to direct cytotoxicity. AC suppressed interleukin-2 (IL-2) and interferon-γ (IFN-γ) production in a concentration-dependent manner. Furthermore, AC decreased the IL-2 and IFN-γ gene expression in primary human T lymphocytes induced by anti-CD3/CD28 Ab. Reporter gene analyses revealed that AC decreased NF-AT-mediated reporter gene expression.Conclusion: AC inhibited T lymphocyte proliferation and decreased the gene expression of IL-2, IFN-γ and NF-AT.

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