Aqueous extract of kan-lu-hsiao-tu-tan ameliorates collagen-induced arthritis in mice by inhibiting oxidative stress and inflammatory responses

Chih Chao Chiang, Yi Rong Li, Kuei Hung Lai, Wei Jen Cheng, Shih Chao Lin, Yi Hsuan Wang, Po Jen Chen, Sien Hung Yang, Chi Chien Lin, Tsong Long Hwang

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Kan-Lu-Hsiao-Tu-Tan (KLHTT) exhibits anti-psoriatic effects through anti-inflammatory activity in mice. However, the therapeutic effects of KLHTT on rheumatoid arthritis (RA), another significant autoimmune inflammatory disorder, have not been elucidated. Herein, we explored the anti-arthritic effects of KLHTT on collagen-induced arthritis (CIA) in mice. Methods: KLHTT was extracted by boiling water and subjected to spectroscopic analysis. Chicken collagen type II (CII) with complete Freund’s adjuvant was intradermally injected to induce CIA in DBA/1J mice. Anti-CII antibody, cytokines, malondialdehyde (MDA), and hydrogen peroxide (H2O2) were measured using ELISA, thiobarbituric acid reactive substances, and a hydrogen peroxide assay kit. Splenocyte proliferation was tested using thymidine incorporation. Th1 and Th17 cells were analyzed by flow cytometry. Results: Oral KLHTT treatment (50 and 100 mg/kg) ameliorated mouse CIA by decreasing the levels of interleukin (IL)-1β, IL-6, IL-17A, and tumour necrosis factor-α in the paw homogenates and serum. KLHTT also suppressed anti-CII antibody formation, splenocyte proliferation, and splenic Th1 and Th17 cell numbers. Additionally, KLHTT showed antioxidant activity by reducing the concentrations of MDA and H2O2 in paw tissues. Conclusions: The therapeutic effects of KLHTT in CIA mice were through regulating oxidative stress and inflammatory responses. Our results suggest that KLHTT has potential to treat RA.

Original languageEnglish
Article number313
Pages (from-to)1-17
Number of pages17
JournalLife
Volume10
Issue number12
DOIs
Publication statusPublished - Dec 2020

Keywords

  • Chinese medicine
  • Collagen-induced arthritis
  • Immunity
  • Inflammation
  • Kan-Lu-Hsiao-Tu-Tan

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Space and Planetary Science
  • Palaeontology

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