Approaching biomarkers of membranous nephropathy from a murine model to human disease

Yuh Feng Lin, Chia Chao Wu, Jin Shuen Chen, Ching Feng Huang, Chun Chi Chen, Kuo Chen Lu, Pauling Chu, Huey Kang Sytwu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background. Membranous glomerulonephropathy (MN) is the most prevalent cause of nephrotic syndrome in adult humans. However, the specific biomarkers of MN have not been fully elucidated. We examined the alterations in gene expression associated with the development of MN. Methods. Murine MN was induced by cationic bovine serum albumin (cBSA). After full-blown MN, cDNA microarray analysis was performed to identify gene expression changes, and highly expressed genes were evaluated as markers both in mice and human kidney samples. Results. MN mice revealed clinical proteinuria and the characteristic diffuse thickening of the glomerular basement membrane. There were 175 genes with significantly different expressions in the MN kidneys compared with the normal kidneys. Four genes, metallothionein-1 (Mt1), cathepsin D (CtsD), lymphocyte 6 antigen complex (Ly6), and laminin receptor-1 (Lamr1), were chosen and quantified. Mt1 was detected mainly in tubules, Lamr1 was highly expressed in glomeruli, and CtsD was detected both in tubules and glomeruli. The high expressions of Lamr1 and CtsD were also confirmed in human kidney biopsies. Conclusion. The murine MN model resembled the clinical and pathological features of human MN and may provide a tool for investigating MN. Applying cDNA microarray analysis may help to identify biomarkers for human MN.

Original languageEnglish
Article number581928
JournalJournal of Biomedicine and Biotechnology
Volume2011
DOIs
Publication statusPublished - 2011

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Laminin Receptors
Membranous Glomerulonephritis
Cathepsin D
Biomarkers
Metallothionein
Genes
Microarrays
Gene expression
Complementary DNA
Lymphocytes
Biopsy
Bovine Serum Albumin
Kidney
Antigens
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Gene Expression
Glomerular Basement Membrane
Nephrotic Syndrome
Proteinuria

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

Approaching biomarkers of membranous nephropathy from a murine model to human disease. / Lin, Yuh Feng; Wu, Chia Chao; Chen, Jin Shuen; Huang, Ching Feng; Chen, Chun Chi; Lu, Kuo Chen; Chu, Pauling; Sytwu, Huey Kang.

In: Journal of Biomedicine and Biotechnology, Vol. 2011, 581928, 2011.

Research output: Contribution to journalArticle

Lin, Yuh Feng ; Wu, Chia Chao ; Chen, Jin Shuen ; Huang, Ching Feng ; Chen, Chun Chi ; Lu, Kuo Chen ; Chu, Pauling ; Sytwu, Huey Kang. / Approaching biomarkers of membranous nephropathy from a murine model to human disease. In: Journal of Biomedicine and Biotechnology. 2011 ; Vol. 2011.
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abstract = "Background. Membranous glomerulonephropathy (MN) is the most prevalent cause of nephrotic syndrome in adult humans. However, the specific biomarkers of MN have not been fully elucidated. We examined the alterations in gene expression associated with the development of MN. Methods. Murine MN was induced by cationic bovine serum albumin (cBSA). After full-blown MN, cDNA microarray analysis was performed to identify gene expression changes, and highly expressed genes were evaluated as markers both in mice and human kidney samples. Results. MN mice revealed clinical proteinuria and the characteristic diffuse thickening of the glomerular basement membrane. There were 175 genes with significantly different expressions in the MN kidneys compared with the normal kidneys. Four genes, metallothionein-1 (Mt1), cathepsin D (CtsD), lymphocyte 6 antigen complex (Ly6), and laminin receptor-1 (Lamr1), were chosen and quantified. Mt1 was detected mainly in tubules, Lamr1 was highly expressed in glomeruli, and CtsD was detected both in tubules and glomeruli. The high expressions of Lamr1 and CtsD were also confirmed in human kidney biopsies. Conclusion. The murine MN model resembled the clinical and pathological features of human MN and may provide a tool for investigating MN. Applying cDNA microarray analysis may help to identify biomarkers for human MN.",
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